RESUMO
Screening for anti-influenza virus activity in compounds isolated from Streptomyces sp. RI18, which was isolated using the membrane filter method, uncovered a novel compound, JBIR-68 (1), which contains a unique skeleton. Its structure was established by extensive NMR and MS analyses. In addition, 1 was synthesized to confirm the configuration of its sugar moiety. Compound 1 inhibited influenza virus growth in plaque assays.
Assuntos
Antivirais/química , Monossacarídeos/química , Orthomyxoviridae/efeitos dos fármacos , Pirimidinonas/química , Streptomyces/química , Antivirais/isolamento & purificação , Antivirais/farmacologia , Estrutura Molecular , Monossacarídeos/isolamento & purificação , Monossacarídeos/farmacologia , Pirimidinonas/isolamento & purificação , Pirimidinonas/farmacologiaRESUMO
We designed a simple and efficient inlet system to act as an interface between samples at atmospheric pressure and the high vacuum inside a mass spectrometer. The newly designed stainless steel orifice leak sample inlet system is simple and rugged and fulfills all the basic requirements. With this inlet system coupled with a lithium ion attachment mass spectrometer, it is possible to detect any chemical species at atmospheric pressure, including radical intermediates, on a real-time basis. For illustrative purposes, the sampling efficiency of the inlet probe coupled with a lithium ion attachment mass spectrometer is discussed for laboratory air and polyethylene pyrolysis.