Modelling and optimization of biodiesel production from waste fish oil using nano immobilized rPichiapastoris whole cell biocatalyst with response surface methodology and hybrid artificial neural network based approach.

In this study, zinc oxide (ZnO) nano particle immobilized recombinant whole cell biocatalyst (rWCB) was used for bioconversion of waste fish oil in to biodiesel in a lab scale packed bed reactor (PBR). Central composite design and hybrid artificial neural network (ANN) models were explored to optimize the production of biodiesel. Developed rWCB exhibited maximum lipase activity at 15 % (v/v) of glutaraldehyde concentration and 6 % (w/v) of ZnO nanoparticles at pH of 7. Maximum biodiesel yield reached about 91.54 ± 1.86 % after 43 h in PBR using hybrid ANN model predicted process conditions of 13.2 % (w/v) of nano immobilized rWCB concentration and 4.7:1 of methanol to oil ratio at 33 °C. Importantly, developed nano immobilized rWCB was adequately stable for commercialization. Thus, production of biodiesel from waste fish oil using ZnO nano immobilized rWCB could become potential candidate for commercialization.

Promising strategies of circular bioeconomy using heavy metal phytoremediated plants - A critical review.

Phytoremediation is an excellent method for removing harmful heavy metals from the environment since it is eco-friendly, uses little energy, and is inexpensive. However, as phytoremediated plants can turn into secondary sources for heavy metals, complete heavy metal removal from phytoremediated plants is necessary. Elimination of toxic heavy metals from phytoremediated plants should be considered with foremost care. This review highlights about important sources of heavy metal contamination, health effects caused by heavy metal contamination and technological breakthroughs of phytoremediation. This review critically emphasis about promising strategies to be engaged for absolute reutilization of heavy metals and spectacular approaches of production of commercially imperative products from phytoremediated plants through circular bioeconomy with key barriers. Thus, phytoremediation combined with circular bioeconomy can create a new platform for the eco-friendly life.

Production of biodiesel from Caulerpa racemosa oil using recombinant Pichia pastoris whole cell biocatalyst with double displayed over expression of Candida antartica lipase.

In this study, Caulerpa racemosa oil was used to produce biodiesel by recombinant Pichia pastoris displaying bound (rPp-BL) and secretory lipase (rPp-SL). Collected algae was pre-treated using ultrasonication, microwave and solvent extraction. Defatted C. racemosa was subjected to dilute acid treatment to obtain algal biomass hydrolysate. Both rPp-BL and rPp-SL were cultivated in algal biomass hydrolysate and glycerol. Surfactant treatment was performed on rPp-BL. Screening and optimization of variables were performed for biodiesel production using Plackett Burman design and central composite design, respectively. About 10.64 % (w/w) of algal oil was extracted from C. racemosa. Both rPp-BL and rPp-SL effectively utilized C. racemosa biomass hydrolysate and glycerol. rPp-SL combined with triton X (1.0 % w/v) treated rPp-BL for 3 min improved lipase activity. Methanol to oil ratio, combined whole cell biocatalyst and temperature were significant factors. Under optimum conditions, biodiesel yield reached about 93.64 % after 30 h using developed whole cell biocatalyst.

Techno economic analysis of malic acid production using crude glycerol derived from waste cooking oil.

In the present work, Aspergillus niger was employed to produce commercially valuable malic acid from crude glycerol derived from waste cooking oil. Crude glycerol dosage, yeast extract dosage and initial pH were the influencing factors playing a significant role in the malic acid synthesis. The optimal condition for malic acid biosynthesis was studied by using response surface methodology. Further the feasibility analysis for biosynthesis of malic acid from crude glycerol was studied using the laboratory scale optimized data, with this experimentally optimized data, plant was simulated using SuperPro Designer (v10). The cost involved for malic acid synthesis per unit volume was likely expected to be $0.43/kg of malic acid using reactive extraction method. Thus, process optimization combined with techno-economical analysis of malic acid production could be beneficial.

Anaerobic biobutanol production from black strap molasses using Clostridium acetobutylicum MTCC11274: Media engineering and kinetic analysis.

Microbial reduction of black strap molasses (BSM) by Clostridium acetobutylicum MTCC 11,274 was performed for the production of biobutanol. The optimum fermentation conditions were predicted using one factor at a time (OFAT) method. The identification of significant parameters was performed using Plackett-Burman Design (PBD). Furthermore the fermentation conditions were optimized using central composite design (CCD). The kinetics of substrate utilization and product formation were investigated. Initial pH, yeast extract concentration (g/L) and total reducing sugar concentration (g/L) were found as significant parameters affecting butanol production using C. acetobutylicum MTCC11274. The maximum butanol production under optimal condition was 10.27 + 0.82 g/L after 24 h. The waste black strap molasses obtained from sugar industry could be used as promising substrate for the production of next generation biofuel.

Advances in bio food packaging - An overview.

In recent years, there has been an increase in demand for bioactive techniques in the food packaging industry. Although edible packaging is popular, it has yet to be effectively implemented into the market. Packaging made of plastics and chemicals is widely employed in the market today, posing a threat to the environment and living creatures. This research attempts to show current breakthroughs and progress in the field of biodegradable packaging. When compared to ancient packaging materials, bio-based packaging materials are safer. Sustainable biodegradable packaging materials can be made from edible films, coatings, and other bio food packaging techniques made from various biological resources. This paper discusses the important qualities and advantages of several bio-based packing materials. It is highlighted the advantages of bio-based packaging materials over synthetic packaging materials. It has been debated the importance of employing bio-based packaging to mitigate the environmental risks associated with traditional packaging technologies. Many researchers may be prompted by this study to focus on packaging reformulation options. Thus, we can attain food packing materials by considering customer's economic and sustainability aspects.

Overview of Current Developments in Biobutanol Production Methods and Future Perspectives.

Renewable biobutanol production is receiving more attention toward substituting fossil-based nonrenewable fuels. Biobutanol is recognized as the top most biofuel with extraordinary properties as compared with gasoline. The demand for biobutanol production is increasing enormously due to application in various industries as chemical substituent. Biobutanol production technology has attracted many researchers toward implementation of replacing cost-effective substrate and easy method to recover from the fermentation broth. Sugarcane bagasse, algal biomass, crude glycerol, and lignocellulosic biomass are potential cost-effective substrates which could replace consistent glucose-based substrates. The advantages and limitations of these substrates have been discussed in this chapter. Moreover, finding the integrated biobutanol recovery methods is an important factor parameter in production of biobutanol. This chapter also concentrated on possibilities and drawbacks of obtainable integrated biobutanol recovery methods. Thus, successful process involving cost-effective substrate and biobutanol recovery methods could help to implementation of biobutanol production industry. Overall, this chapter has endeavored to increase the viability of industrial production of biobutanol.

Lipid bioproduction from delignified native grass (Cyperus distans) hydrolysate by Yarrowia lipolytica.

In the present study, native grass (Cyperus distans) was utilized for the production of lipid using Yarrowia lipolytica MTCC 9519. Initially, pretreatment methods using hydrothermal and alkaline delignification were performed to obtain cellulose rich liquid fractions. Delignified native grass biomass was enzymatically hydrolyzed to convert non fermentable sugars in to fermentable sugars. The growth of Y. lipolytica MTCC 9519 by utilizing pretreated native grass hydrolysate was evaluated. The yield and concentration of total reducing sugars after enzyme hydrolysis were found to be 378 ± 35 mg/g of pretreated biomass and 28.64 g/L ± 1.25 g/L, respectively. When pretreated, delignified native grass hydrolysate was used with (NH4)2SO4 (30C/N ratio) and sodium n-octanoate (0.4% w/w), the dry cell weight and lipid accumulation of Y. lipolytica MTCC 9519 reached about 19.88 ± 1.54 g/L and 53.62% (w/w) respectively after 96 h. Thus, native grass could become a promising substrate for biolipid production.

Enhanced malic acid production using Aspergillus niger coupled with in situ product recovery.

In the present investigation, recovery of malic acid from the fermentation broth was performed by using in situ reactive extraction method employing different combination of amine and solvent systems. Totally six solvent mixtures were tested for toxicity on Aspergillus niger PJR1. Further, effect of solvent mixture concentration on separation of malic acid was investigated. Solvent system consisting of 2 M of trioctylamine in 1-octanol was found to be non-toxic to A. niger PJR1 and resulted in the maximum partition coefficient of 0.75 when the solvent mixture to liquid ratio of 1:2 used. A. niger PJR1 from crude glycerol using in situ reactive batch fermentation resulted in the maximum malic acid titer of 115.67 ± 3.5 g/L with the productivity of 0.53 g/L.h after 216 h. Further, fed batch extractive fermentation with crude glycerol resulted in malic acid titer of 131.48 ± 3.4 g/L with the productivity of 0.45 g/L.h after 288 h. Thus reactive extraction combined with in situ fermentation could become effective method for enhanced malic acid production.

Biodiesel production from microalgae Nannochloropsis oculata using heterogeneous Poly Ethylene Glycol (PEG) encapsulated ZnOMn2+ nanocatalyst.

In this present work nanocomposite composed of Mn-ZnO capped with Poly Ethylene Glycol (PEG) was utilized as heterogeneous catalyst for the transesterification of oil extracted from Nannochloropsis oculata into biodiesel using methanol as an acyl acceptor. The synthesized Mn-ZnO novel nanocomposite capped with Poly Ethylene Glycol (PEG) was characterized by using SEM and XRD. Lipid contents from the microalgae were extracted by sonication and biphasic solvent method. The process parameters involved for heterogeneous catalysis of N. oculata to biodiesel were optimized and found to be oil to methanol molar ratio of 1:15 (mol:mol), catalyst loading 3.5% (w/w) and reaction temperature of 60 °C for 4 h of reaction time by Response Surface Method. The reusability studies showed that the nano-catalyst can be reused efficiently for 4 cycles. The yield of biodiesel obtained from N. oculata species using Mn-ZnO nanocomposite capped with PEG was 87.5%.

Process optimization and kinetic analysis of malic acid production from crude glycerol using Aspergillus niger.

In the present work, optimization of crude glycerol fermentation to produce malic acid by using Aspergillus niger was investigated using response surface methodology and artificial neural network. Kinetic investigation of bioconversion of crude glycerol into malic acid using Aspergillus niger was studied using Monod, Mosser, and Haldane-Andrew models. Crude glycerol concentration, initial pH and yeast extract concentration were found to be significant compounds affecting malic acid production by Aspergillus niger. Both dry cell weight and malic acid titre were found decreased with increase in crude glycerol concentration. Haldane-Andrew model gave the best fit for the production of malic acid from crude glycerol with µmax of 0.1542 h-1. The maximum malic acid production obtained under optimum conditions was 92.64 + 1.54 g/L after 192 h from crude glycerol using Aspergillus niger.

Malic acid production from biodiesel derived crude glycerol using morphologically controlled Aspergillus niger in batch fermentation.

In the present investigation, the effects of crude glycerol concentration, spore inoculum concentration, yeast extract concentration and shaking frequency on seed morphology of Aspergillus niger PJR1 on malic acid production were investigated and dispersed fungal mycelium with higher biomass (20.25 ±â€¯0.91 g/L) was obtained when A. niger PJR1 grow on crude glycerol. Dry cell weight under dispersed fermentation was 21.28% higher than usual pellet fermentation. The optimal crude glycerol, nitrogen source and nitrogen source concentration were found to be 160 g/L, yeast extract and 1.5 g/L, respectively. Batch fermentation in a shake flask culture containing 160 g/L crude glycerol resulted in the yield of malic acid 83.23 ±â€¯1.86 g/L, after 192 h at 25 °C. Results revealed that morphological control of A. niger is an efficient method for increased malic acid production when crude glycerol derived from biodiesel production is used as feedstock.

Malic acid production by chemically induced Aspergillus niger MTCC 281 mutant from crude glycerol.

In the present investigation, crude glycerol derived from transesterification process was utilized to produce the commercially-valuable malic acid. A combined resistant on methanol and malic acid strain of Aspergillus niger MTCC 281 mutant was generated in solid medium containing methanol (1-5%) and malic acid (40-80 g/L) by the adaptation process for 22 weeks. The ability of induced Aspergillus niger MTCC 281 mutant to utilize crude glycerol and pure glycerol to produce malic acid was studied. The yield of malic acid was increased with 4.45 folds compared with that of parent strain from crude glycerol. The highest concentration of malic acid from crude glycerol by using beneficial mutant was found to be 77.38 ±â€¯0.51 g/L after 192 h at 25 °C. This present study specified that crude glycerol by-product from biodiesel production could be used for producing high amount of malic acid without any pretreatment.