RESUMO
The distribution of the glycoprotein, mucin 1 (MUC1), was determined in lactating guinea-pig mammary tissue at the resolution of the electron microscope. MUC1 was detected on the apical plasma membrane of secretory epithelial cells, the surface of secreted milk-fat globules, the limiting membranes of secretory vesicles containing casein micelles and in small vesicles and tubules in the apical cytoplasm. Some of the small MUC1-containing vesicles were associated with the surfaces of secretory vesicles and fat droplets in the cytoplasm. MUC1 was detected in much lower amounts on basal and lateral plasma membranes. By quantitative immunocytochemistry, the ratio of MUC1 on apical membranes and milk-fat globules to that on secretory vesicle membranes was estimated to be 9.2:1 (density of colloidal gold particles/microm membrane length). The ratio of MUC1 on apical membranes compared with basal/lateral membranes was approximately 99:1. The data are consistent with a mechanism for milk-fat secretion in which lipid globules acquire an envelope of membrane from the apical surface and possibly from small vesicles containing MUC1 in the cytoplasm. During established lactation, secretory vesicle membrane does not appear to contribute substantially to the milk-fat globule membrane, or to give rise in toto to the apical plasma membrane.
Assuntos
Mama/metabolismo , Glicolipídeos/metabolismo , Glicoproteínas/metabolismo , Lactação/fisiologia , Mucina-1/metabolismo , Animais , Anticorpos Monoclonais/imunologia , Sítios de Ligação , Polaridade Celular/fisiologia , Concanavalina A/metabolismo , Células Epiteliais/metabolismo , Epitopos/imunologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Cobaias , Imuno-Histoquímica , Gotículas Lipídicas , Microscopia Eletrônica , Gravidez , Receptores de Concanavalina A/metabolismo , Distribuição TecidualRESUMO
This experiment tested the hypothesis that thyroid hormones are essential for a milk production response to growth hormone (GH) and prolactin (PRL). Prior to breeding, female transgenic mice expressing the herpes simplex type-I thymidine kinase in the thyroid were treated with ganciclovir to ablate thyroid follicular cells. To provide for normal gestation, thyrocyte-ablated mice were supplied thyroxine (T4) in drinking water (0.2 microgram/ml) until 7 days before parturition. Litter size was adjusted to 9 pups, hormone administration began on Day 2 of lactation, and mice were sacrificed on Day 12. There were 5-6 mice in each of 7 treatments that included nonablated controls, thyrocyte-ablated controls, and thyrocyte-ablated mice treated with T4, GH, PRL, GH + T4, and PRL + T4. Thyroxine was administered in drinking water, and GH and PRL (20 microgram/d) were administered by subcutaneous injection. Compared with thyrocyte-ablated controls, litter weight gain was unaffected when dams were treated with GH, PRL, or T4 alone. However, when dams were treated with GH or PRL in combination with T4, litter weight gain increased 13% compared with thyrocyte-ablated controls and 18% compared with GH or PRL-treated mice. Concentration of T4 in serum of pups averaged 62 ng/ml and did not differ among treatments. Concentration of T4 in serum of dams averaged 76 ng/ml when T4-treated. Thyroxine 5'-deiodinase (5'D), the enzyme that converts T4 to triiodothyronine, was quantitated in liver, kidney, and mammary gland. Quantity of 5'D was lower in liver and kidney of thyrocyte-ablated dams without T4 than in respective tissues of mice treated with T4, and there was no effect of GH or PRL. However, in mammary gland, 5'D was increased by treatment with GH, PRL, or T4. Data show that thyroid hormones are necessary for a galactopoietic response to GH and PRL and demonstrate a unique organ-specific regulation of 5'D by galactopoietic hormones.
Assuntos
Hormônio do Crescimento/farmacologia , Lactação/efeitos dos fármacos , Prolactina/farmacologia , Hormônios Tireóideos/farmacologia , Animais , Animais Recém-Nascidos , Peso Corporal/efeitos dos fármacos , Feminino , Ganciclovir/farmacologia , Iodeto Peroxidase/análise , Camundongos , Camundongos Transgênicos , Timidina Quinase/genética , Tireoidectomia , Tiroxina/sangue , Tiroxina/farmacologiaRESUMO
We investigated the expression of butyrophilin in eukaryotic cells with a view to determining the number of mRNA species, the incorporation of the peptide chain into microsomes, and the topology of the processed protein in biological membranes. Butyrophilin is synthesized from a single sized mRNA in both bovine and murine lactating mammary tissue and associates with microsomal membranes with a type I topology (Nexo.Ccyto) via a single hydrophobic anchor in the middle of the sequence. Several isoelectric variants of the protein were detected in cellular membranes from lactating bovine mammary tissue and in the milk-fat-globule membrane. We found no evidence for soluble forms of butyrophilin in postmicrosomal supernatants. The 66-kDa protein appears to be subjected to limited proteolysis, giving rise to a 62-kDa fragment lacking the C terminus and to other more minor fragments of lower Mr in the milk-fat-globule membrane. Antipeptide antibodies to epitopes within the N- and C-terminal domains were used to show that butyrophilin retains a type I topology in plasma membranes when expressed in insect cells from a baculovirus vector, and in secreted milk-fat globules. These data do not agree with previous suggestions that butyrophilin may exist in cytoplasmic soluble forms, or be reorganized in the plane of the lipid bilayer during secretion in lipid droplets from mammary cells. The results are discussed with reference to the role butyrophilin may play as the principal scaffold for the assembly of a complex with xanthine oxidase and other proteins that functions in the budding and release of milk-fat globules from the apical surface during lactation.
Assuntos
Glândulas Mamárias Animais/química , Glicoproteínas de Membrana/química , RNA Mensageiro/análise , Animais , Butirofilinas , Bovinos , Clonagem Molecular , Imunofluorescência , Expressão Gênica/genética , Immunoblotting , Glicoproteínas de Membrana/análise , Glicoproteínas de Membrana/metabolismo , Camundongos , Proteínas do Leite/química , Proteínas do Leite/metabolismo , Fragmentos de Peptídeos/análise , Biossíntese de Proteínas/genética , Análise de Sequência , Spodoptera/genéticaRESUMO
Previous in situ hybridization studies from our laboratory have shown that expression of certain milk protein genes, e.g. alpha-lactalbumin, is very high in most parts of the mammary glands of sheep and cattle, while in other areas containing an abundance of fat globules it is virtually zero (Molenaar et al., 1992). One possible explanation is that some areas of the mammary gland are dedicated to protein synthesis and some to fat synthesis. To check this possibility, the cRNA for butyrophilin, a milk-fat globule membrane protein, and hence a putative marker of milk fat synthesis, was used as a probe in in situ hybridization studies. The results show quite clearly that the patterns of expression for this gene are similar, cell type for cell type, as those for milk protein genes such as alpha-lactalbumin and alpha S1casein. In addition, we found that butyrophilin gene expression more closely matches that of alpha S1casein than that of alpha-lactalbumin. If it is shown in the future that butyrophilin is indeed a marker for milk fat synthesis, then these results support the current assumption that fat and protein synthesis do occur in the same cell.
Assuntos
Caseínas/biossíntese , Caseínas/genética , Expressão Gênica/fisiologia , Lipídeos/biossíntese , Glicoproteínas de Membrana/biossíntese , Glicoproteínas de Membrana/genética , Proteínas do Leite/biossíntese , Proteínas do Leite/genética , Leite/metabolismo , Animais , Butirofilinas , Feminino , Glândulas Mamárias Animais/metabolismo , Hibridização de Ácido Nucleico , Sondas RNA , RNA Mensageiro/biossíntese , OvinosRESUMO
Thyroxine 5'-deiodinase (5'D) catalyses deiodination of the prohormone thyroxine (T4) to the metabolically active hormone 3,5,3'-tri-iodothyronine (T3). Previously, it has been demonstrated that rat mammary gland expresses a 5'D with enzymatic properties equivalent to those of the type I enzyme (5'D-I) found in rat liver and kidney. Using complementary DNA (cDNA) for rat hepatic 5'D-I, we have examined expression of 5'D-I messenger RNA (mRNA) in liver, and mammary gland from virgin and lactating rats, and in seven other tissues from virgin rats. 5'D-I mRNA could not be detected in mammary gland either by Northern blotting or by the more sensitive technique of reverse transcribing mRNA and then amplifying the cDNA by polymerase chain reaction (RT-PCR). Analysis of the seven tissues from virgin rats by RT-PCR showed 5'D-I amplicons in liver, kidney and thyroid. No amplicons were detected in adrenal gland, cardiac muscle, skeletal muscle or spleen. In addition, the effect of lactation intensity on circulating thyroid hormones, hepatic and mammary gland 5'D activity, and hepatic 5'D-I mRNA levels was examined. A strong inverse relationship was noted between increased lactation intensity (suckling burden) and circulating T4 and T3, hepatic 5'D-I activity and hepatic 5'D-I mRNA levels. Mammary gland 5'D activity was positively correlated to lactation intensity. The data presented strongly suggest that the 5'D activity expressed in lactating mammary gland is encoded by a mRNA different from the 5'D-I message found in rat liver, kidney and thyroid gland, and may help explain the differential regulation of 5'D-I activity in these organs during lactation. In addition, hepatic 5'D-I activity was found to be correlated with the concentration of 5'D-I mRNA, suggesting that regulation is pretranslational. Results are consistent with a previously suggested involvement of 5'D in establishing metabolic adaptations to support lactation.
Assuntos
Iodeto Peroxidase/metabolismo , Lactação/metabolismo , Fígado/enzimologia , Glândulas Mamárias Animais/enzimologia , Animais , Sequência de Bases , Northern Blotting , Feminino , Regulação da Expressão Gênica/fisiologia , Iodeto Peroxidase/genética , Rim/enzimologia , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Gravidez , RNA Mensageiro/análise , Ratos , Ratos Sprague-Dawley , Glândula Tireoide/enzimologia , Tiroxina/sangue , Distribuição Tecidual , Tri-Iodotironina/sangueRESUMO
Twenty-four Holstein cows, producing at least 21 kg of milk/d, were used in two replicate experiments to determine the effect of presence or absence of pulsation on loss of teat canal keratin during machine milking. Left quarters were milked without pulsation and right quarters were milked with pulsation. On d 0 and 10, keratin was collected from one left and from one right teat canal of each cow prior to milking and from the remaining two teat canals after milking. Milk was collected for assessment of SCC and bacteriological status on d 0 and approximately every 3 d until d 18. Quantity of keratin recovered before milking on d 10 did not differ between teats milked with or without pulsation, but loss of keratin because of milking was greater from teats milked with pulsation. By d 7, 30% (12 of 43) of quarters milked without pulsation had become infected, but no (0 of 47) quarters milked with pulsation were infected. By d 14 to 16, new infections had increased to 68% (28 of 41) of quarters milked without pulsation and 2% (1 of 43) in quarters milked with pulsation; mean SCC in pulsationless quarters increased sevenfold relative to pulsation quarters. Protein and water content of keratin did not differ because of treatment, and changes in lipid composition were minor. Histological analysis of the teats of 4 cows indicated that the mean diameter of the teat canal, within 2 h after milking, was greater without pulsation than with pulsation (680 vs. 483 microns).
Assuntos
Bovinos/fisiologia , Indústria de Laticínios/métodos , Queratinas/metabolismo , Lactação , Glândulas Mamárias Animais/metabolismo , Mastite Bovina/epidemiologia , Animais , Feminino , Queratinas/análise , Queratinas/química , Glândulas Mamárias Animais/patologia , Mastite Bovina/patologia , Peso MolecularRESUMO
The molecular and cellular biology of the milk protein butyrophilin is reviewed. Butyrophilin constitutes more than 40% by weight of the total protein associated with the fat globule membrane of bovine milk. Closely related proteins are abundant in the fat globule membranes of many other species. Butyrophilin is synthesized as a peptide of 526 amino acids with an amino-terminal hydrophobic signal sequence of 26 amino acids, which is cleaved before secretion in association with the fat globule membrane. Hydropathy analysis and in vitro translation of butyrophilin mRNA indicate that the protein associates with membranes in a type I orientation via a single stretch of 27 hydrophobic amino acids in the approximate middle of the sequence. Evidence that butyrophilin is incorporated into fat globule membrane as a transmembrane protein and as a cytoplasmically oriented peripheral component is discussed. The carboxy-terminal sequence of butyrophilin is significantly homologous to two other proteins: ret finger protein and the 52-kDa nuclear antigen A of Sjögren's syndrome. Expression of bovine butyrophilin mRNA correlates with the onset of milk fat secretion toward the end of pregnancy and is maintained throughout lactation. The possible function of butyrophilin in the secretion of milk lipid droplets is discussed.
Assuntos
Glicoproteínas de Membrana , Leite/química , Sequência de Aminoácidos , Animais , Butirofilinas , Bovinos , Glândulas Mamárias Animais/química , Glândulas Mamárias Animais/metabolismo , Glândulas Mamárias Animais/ultraestrutura , Glicoproteínas de Membrana/análise , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/fisiologia , Dados de Sequência Molecular , Homologia de Sequência , Distribuição TecidualRESUMO
During a search for novel coding sequences within the human MHC class I region (chromosome 6p21.3), we found an exon (named B30-2) coding for a 166-amino-acid peptide which is very similar to the C-terminal domain of several coding sequences: human 52-kD Sjögren's syndrome nuclear antigen A/Ro (SS-A/Ro) and ret finger protein (RFP), Xenopus nuclear factor 7 (XNF7), and bovine butyrophilin. The first three of these proteins share similarities over the whole length of the molecule whereas butyrophilin is similar in the C-terminal domain. The N-terminal domain of butyrophilin is similar to rat myelin/oligodendrocyte glycoprotein (MOG) and chicken B blood group system (B-G) protein. These domains are components of a new subfamily of the immunoglobulin superfamily (IgSF). Butyrophilin is thus a mosaic protein composed of the MOG/B-G Ig-like domain and the C-terminal domain of 52-kD SS-A/Ro, RFP, and XNF7 (B30-2-like domain). Moreover, in situ hybridization shows that RFP, butyrophilin, and MOG map to the human chromosome 6p21.3-6p22 region and are thus close to the MHC class I genes. It is therefore possible that the butyrophilin gene is the product of an exon shuffling event which occurred between ancestors of the RFP and MOG genes. To our knowledge, this is the first example of the colocalization of a chimeric gene and its putative progenitors. Finally, regulatory protein T-lymphocyte 1 (Rpt-1) shares similarities with the N-terminal halves of RFP, 52-kD SS-A/Ro, and XNF7, but not with the B30-2-like domain. We show that the ancestral Rpt-1 gene evolved by overprinting.
Assuntos
Evolução Biológica , Cromossomos Humanos Par 6 , Proteínas de Ligação a DNA , Genes MHC Classe I , Família Multigênica , Sequência de Aminoácidos , Sequência de Bases , Butirofilinas , DNA , Éxons , Humanos , Glicoproteínas de Membrana/genética , Dados de Sequência Molecular , Proteínas Nucleares/genética , Mapeamento por Restrição , Homologia de Sequência de AminoácidosRESUMO
Butyrophilin is a glycoprotein expressed on the apical surfaces of secretory cells in lactating mammary tissue, which may function in the secretion of milk-fat droplets (Franke, W. W., Heid, H. W., Grund, C., Winter, S., Freudenstein, C., Schmid, E., Jarasch, E.-D., and Keenan, T. W. (1981) J. Cell Biol. 89, 485-494). A cDNA clone encoding bovine butyrophilin was isolated and the primary structure of the protein deduced from the DNA sequence. Bovine butyrophilin contains 526 amino acids with a putative signal peptide of 26 amino acids. Hydropathy analysis predicts the existence of a single membrane-spanning region with the amino terminus facing the exoplasmic space. Butyrophilin cDNA hybridized to mRNA of 2.9 kilobases in bovine mammary tissue taken from pregnant or lactating animals, but specific mRNA was not detected in a 2-year-old virgin cow. The amount of message was maximal in lactating tissue. Butyrophilin mRNA could not be detected in bovine heart, intestine, kidney, liver, ovary, or uterus. A search of the GenBank and EMBL data banks showed closest homology was between the C termini of butyrophilin and "ret finger protein" (Takahashi, M., Inaguma, Y., Hiai, H., and Hirose, F. (1988) Mol. Cell. Biol. 8, 1853-1856). The ret-finger protein gene is expressed in a variety of tumor cell lines, mouse testis and embryonic tissue, which like the mammary gland undergo periods of rapid cell division and development. The possible significance of this homology and the possible function of butyrophilin in milk-lipid secretion are discussed.
Assuntos
Glândulas Mamárias Animais/metabolismo , Glicoproteínas de Membrana/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Butirofilinas , Bovinos , Clonagem Molecular , DNA/genética , Feminino , Biblioteca Gênica , Lactação , Glândulas Mamárias Animais/fisiologia , Glicoproteínas de Membrana/metabolismo , Dados de Sequência Molecular , Mucina-1 , Gravidez , Conformação Proteica , RNA Mensageiro/análise , RNA Mensageiro/genética , Mapeamento por Restrição , Homologia de Sequência do Ácido NucleicoRESUMO
A procedure for the preparation of affinity-purified antibody is described. Protein mixtures are separated by electrophoresis in sodium dodecyl sulfate (SDS)--polyacrylamide gels. Individual bands of protein are cut from the gel and fixed in situ with glutaraldehyde. The gel pieces are then homogenized and washed extensively with buffered solutions and chaotropic agents. The washed gels can then be used as immunoadsorbents to purify antibodies from crude antisera. This method should be especially useful for the preparation of small amounts of antibody to proteins that are difficult to purify by conventional means, that are available only in limited quantity, or that cannot be blotted to immunoadsorbents such as nitrocellulose or diazotized paper.