Agronomic Factors Associated with Bacterial Leaf Streak Development Caused by Xanthomonas vasicola pv. vasculorum in Corn.

In 2016, the bacterium Xanthomonas vasicola pv. vasculorum was reported causing bacterial leaf streak on Nebraska corn. Prior to this report, the pathogen was reported on corn only in South Africa. Hence, there was a lack of information about the effects of corn production practices on bacterial leaf streak development. A survey of growers, crop consultants, extension agents, and others in the field of agriculture was initiated during the 2016 and 2017 corn growing seasons to examine agronomic factors that may affect the disease. Survey respondents were asked to submit a symptomatic leaf sample for pathogen confirmation via polymerase chain reaction, along with a completed survey on corn production practices. Analyses of 325 survey responses via random forest analysis indicated that irrigation, planting date, and crop rotation were the three most important predictors of corn samples testing positive for X. vasicola pv. vasculorum. According to a classification and regression tree analysis, irrigation use, the V7-R2 range of crop stages, multiple years of corn in a crop rotation or a corn-sorghum rotation, and planting dates after 2 May, were most closely associated with corn samples testing positive for X. vasicola pv. vasculorum. χ2 tests of independence indicated that applications of nitrogen fertilizer and glyphosate herbicide use may also be related to bacterial leaf streak development.

Alternative Hosts in the Families Poaceae and Cyperaceae for Xanthomonas vasicola pv. vasculorum, Causal Agent of Bacterial Leaf Streak of Corn.

The bacterial pathogen Xanthomonas vasicola pv. vasculorum was first reported in the United States causing bacterial leaf streak on Nebraska corn (Zea mays) in 2016. The bacterium is also known to cause disease in sugarcane, grain sorghum, broom bamboo, and various palm species. The objective of this study was to identify alternative hosts for X. vasicola pv. vasculorum among plants commonly found in corn growing areas of the United States. In repeated greenhouse experiments, 53 species of plants found in the United States that had not been tested previously for susceptibility to X. vasicola pv. vasculorum were inoculated with the pathogen and monitored for symptom development. Eleven species in the family Poaceae exhibited symptoms: oat (Avena sativa), rice (Oryza sativa), orchardgrass (Dactylis glomerata), indiangrass (Sorghastrum nutans), big bluestem (Andropogon gerardii), little bluestem (Schizachyrium scoparium), timothy (Phleum pratense), sand bluestem (Andropogon hallii), green foxtail (Setaria viridis), bristly foxtail (Setaria verticillata), and johnsongrass (Sorghum halepense). Yellow nutsedge (Cyperus esculentus) in the Cyperaceae also was a symptomatic host. In addition, endophytic colonization by X. vasicola pv. vasculorum was found in three asymptomatic alternative hosts: downy brome (Bromus tectorum), tall fescue (Festuca arundinacea), and western wheatgrass (Pascopyum smithii). Experiments were also conducted in the field to determine the potential for alternative hosts to become infected by natural inoculum. Symptoms developed only in big bluestem and bristly foxtail in field experiments. These results suggest that infection of alternative hosts by X. vasicola pv. vasculorum can occur, but infection rates might be limited by environmental conditions.

Detection and Characterization of Xanthomonas vasicola pv. vasculorum (Cobb 1894) comb. nov. Causing Bacterial Leaf Streak of Corn in the United States.

Bacterial leaf streak of corn (Zea mays) recently reached epidemic levels in three corn-growing states, and has been detected in another six states in the central United States. Xanthomonas vasicola was identified as the causal agent of this disease. A multilocus sequence alignment of six housekeeping genes and comparison of average nucleotide identity from draft genome sequence were used to confirm phylogenetic relationships and classification of this bacteria relative to other X. vasicola strains. X. vasicola isolates from Nebraska and South Africa were highly virulent on corn and sugarcane and less virulent on sorghum but caused water-soaking symptoms that are typical of X. vasicola infection on the leaves of all three hosts. Based on host range and phylogenetic comparison, we propose the taxonomic designation of this organism to X. vasicola pv. vasculorum ( Cobb 1894 ) comb. nov. Polymerase chain reaction-based diagnostic assays were developed that distinguish X. vasicola pv. vasculorum and X. vasicola pv. holcicola from each other and from other Xanthomonas spp.