RESUMO
Wounding induces a calcium wave and disrupts the calcium gradient across the epidermis but mechanisms mediating calcium and downstream signalling, and longer-term wound healing responses are incompletely understood. As expected, live-cell confocal imaging of Fluo-4-loaded normal human keratinocytes showed an immediate increase in [Ca2+ ]i at the wound edge that spread as a calcium wave (8.3 µm/s) away from the wound edge with gradually diminishing rate of rise and amplitude. The amplitude and area under the curve of [Ca2+ ]i flux was increased in high (1.2 mM) [Ca2+ ]o media. 18α-glycyrrhetinic acid (18αGA), a gap-junction inhibitor or hexokinase, an ATP scavenger, blocked the wound-induced calcium wave, dependent in part on [Ca2+ ]o . Wounding in a high [Ca2+ ]o increased nuclear factor of activated T-cells (NFAT) but not NFkB activation, assessed by dual-luciferase receptor assays compared to unwounded cells. Treatment with 18αGA or the store-operated channel blocker GSK-7975A inhibited wound-induced NFAT activation, whereas treatment with hexokinase did not. Real-time cell migration analysis, measuring wound closure rates over 24 h, revealed that 18αGA essentially blocked wound closure whereas hexokinase and GSK-7975A showed relatively minimal effects. Together these data indicate that while both gap-junction communication and ATP release from damaged cells are important in regulating the wound-induced calcium wave, long-term transcriptional and functional responses are dominantly regulated by gap-junction communication.
Assuntos
Sinalização do Cálcio/fisiologia , Cálcio/metabolismo , Junções Comunicantes/metabolismo , Fatores de Transcrição NFATC/metabolismo , Cicatrização/fisiologia , Trifosfato de Adenosina/metabolismo , Animais , Movimento Celular/fisiologia , Células Cultivadas , Humanos , Queratinócitos/metabolismoRESUMO
Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal papilla cells (DPC) restores the intact DPC transcriptional signature and significantly enhances the efficiency of HF differentiation in HSCs. Furthermore, vascularization of hair-bearing HSCs prior to engraftment allows for efficient human hair growth in immunodeficient mice. The ability to regenerate an entire HF from cultured human cells will have a transformative impact on the medical management of different types of alopecia, as well as chronic wounds, which represent major unmet medical needs.
Assuntos
Alopecia/terapia , Derme/citologia , Folículo Piloso/crescimento & desenvolvimento , Folículo Piloso/transplante , Engenharia Tecidual/métodos , Alopecia/patologia , Animais , Biomimética , Diferenciação Celular , Células Cultivadas , Folículo Piloso/citologia , Células Endoteliais da Veia Umbilical Humana , Humanos , Fator 1 de Ligação ao Facilitador Linfoide/biossíntese , Masculino , Camundongos , Camundongos Nus , Transplante HeterólogoRESUMO
In the hair follicle, the dermal papilla (DP) and dermal sheath (DS) support and maintain proliferation and differentiation of the epithelial stem cells that produce the hair fibre. In view of their regulatory properties, in this study, we investigated the interaction between hair follicle dermal cells (DP and DS) and embryonic stem cells (ESCs); induced pluripotent stem cells (iPSCs); and haematopoietic stem cells. We found that coculture of follicular dermal cells with ESCs or iPSCs supported their prolonged maintenance in an apparently undifferentiated state as established by differentiation assays, immunocytochemistry, and RT-PCR for markers of undifferentiated ESCs. We further showed that cytokines that are involved in ESC support are also expressed by cultured follicle dermal cells, providing a possible explanation for maintenance of ES cell stemness in cocultures. The same cytokines were expressed within follicles in situ in a pattern more consistent with a role in follicle growth activities than stem cell maintenance. Finally, we show that cultured mouse follicle dermal cells provide good stromal support for haematopoiesis in an established coculture model. Human follicular dermal cells represent an accessible and readily propagated source of feeder cells for pluripotent and haematopoietic cells and have potential for use in clinical applications.
RESUMO
Two theories address the origin of repeating patterns, such as hair follicles, limb digits, and intestinal villi, during development. The Turing reaction-diffusion system posits that interacting diffusible signals produced by static cells first define a prepattern that then induces cell rearrangements to produce an anatomical structure. The second theory, that of mesenchymal self-organisation, proposes that mobile cells can form periodic patterns of cell aggregates directly, without reference to any prepattern. Early hair follicle development is characterised by the rapid appearance of periodic arrangements of altered gene expression in the epidermis and prominent clustering of the adjacent dermal mesenchymal cells. We assess the contributions and interplay between reaction-diffusion and mesenchymal self-organisation processes in hair follicle patterning, identifying a network of fibroblast growth factor (FGF), wingless-related integration site (WNT), and bone morphogenetic protein (BMP) signalling interactions capable of spontaneously producing a periodic pattern. Using time-lapse imaging, we find that mesenchymal cell condensation at hair follicles is locally directed by an epidermal prepattern. However, imposing this prepattern's condition of high FGF and low BMP activity across the entire skin reveals a latent dermal capacity to undergo spatially patterned self-organisation in the absence of epithelial direction. This mesenchymal self-organisation relies on restricted transforming growth factor (TGF) ß signalling, which serves to drive chemotactic mesenchymal patterning when reaction-diffusion patterning is suppressed, but, in normal conditions, facilitates cell movement to locally prepatterned sources of FGF. This work illustrates a hierarchy of periodic patterning modes operating in organogenesis.
Assuntos
Folículo Piloso/embriologia , Fator de Crescimento Transformador beta/fisiologia , Animais , Padronização Corporal , Diferenciação Celular , Feminino , Perfilação da Expressão Gênica , Masculino , Camundongos , Camundongos Endogâmicos , Transdução de Sinais , Pele/citologia , Pele/embriologia , Pele/metabolismo , Fator de Crescimento Transformador beta/metabolismoRESUMO
Dermal cell populations are markedly heterogeneous, and they have the capacity to differentiate into dynamic and complex dermal cell compartments. However, the regulatory processes that govern the establishment of each dermal subset remain unknown. Mastrogiannaki et al. provide evidence of Wnt/ß-catenin signaling controlling adipogenic differentiation in the developing reticular dermis. They also show that overexpression of localized Wnt converts dermal adipose cells into a distinct fibroblast subtype, which leads to fibrosis and disrupted hair follicle cycling. These findings highlight the multifaceted roles of Wnt signaling in the normal development and pathology of skin, including the establishment of dermal identity. Further understanding of Wnt involvement and uncovering the roles of specific Wnt ligands could be useful for discovering new therapeutic targets in treating fibrosis-related disorders.
Assuntos
Derme/metabolismo , Fibroblastos/citologia , Proteínas Wnt/genética , Via de Sinalização Wnt/genética , Animais , Diferenciação Celular/genética , Células Cultivadas , Derme/fisiopatologia , Epiderme/metabolismo , Epiderme/fisiopatologia , Fibroblastos/patologia , Regulação da Expressão Gênica , Humanos , Camundongos , Camundongos Transgênicos , Sensibilidade e EspecificidadeRESUMO
During development, multipotent progenitor cells establish lineage-specific programmers of gene activation and silencing underlying their differentiation into specialized cell types. We show that the Polycomb component Cbx4 serves as a critical determinant that maintains the epithelial identity in the developing epidermis by repressing nonepidermal gene expression programs. Cbx4 ablation in mice results in a marked decrease of the epidermal thickness and keratinocyte (KC) proliferation associated with activation of numerous neuronal genes and genes encoding cyclin-dependent kinase inhibitors (p16/p19 and p57). Furthermore, the chromodomain- and SUMO E3 ligase-dependent Cbx4 activities differentially regulate proliferation, differentiation, and expression of nonepidermal genes in KCs. Finally, Cbx4 expression in KCs is directly regulated by p63 transcription factor, whereas Cbx4 overexpression is capable of partially rescuing the effects of p63 ablation on epidermal development. These data demonstrate that Cbx4 plays a crucial role in the p63-regulated program of epidermal differentiation, maintaining the epithelial identity and proliferative activity in KCs via repression of the selected nonepidermal lineage and cell cycle inhibitor genes.
Assuntos
Linhagem da Célula , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Epitélio/metabolismo , Complexo Repressor Polycomb 1/metabolismo , Ubiquitina-Proteína Ligases/metabolismo , Animais , Diferenciação Celular , Proliferação de Células , Epitélio/crescimento & desenvolvimento , Ligases , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Complexo Repressor Polycomb 1/deficiência , Complexo Repressor Polycomb 1/genética , Células-Tronco/citologia , Células-Tronco/metabolismo , Ubiquitina-Proteína Ligases/deficiência , Ubiquitina-Proteína Ligases/genéticaRESUMO
Recent literature suggests that the layer of adipocytes embedded in the skin below the dermis is far from being an inert spacer material. Instead, this layer of dermal white adipose tissue (dWAT) is a regulated lipid layer that comprises a crucial environmental defense. Among all the classes of biological molecules, lipids have the lowest thermal conductance and highest insulation potential. This property can be exploited by mammals to reduce heat loss, suppress brown adipose tissue activation, reduce the activation of thermogenic programs, and increase metabolic efficiency. Furthermore, this layer responds to bacterial challenge to provide a physical barrier and antimicrobial disinfection, and its expansion supports the growth of hair follicles and regenerating skin. In sum, this dWAT layer is a key defensive player with remarkable potential for modifying systemic metabolism, immune function, and physiology. In this review, we discuss the key literature illustrating the properties of this recently recognized adipose depot.
Assuntos
Gordura Subcutânea/fisiologia , Termogênese , Adipócitos Brancos/fisiologia , Adiposidade , Animais , Derme/fisiologia , Folículo Piloso/fisiologia , HumanosRESUMO
Since the first mammal was cloned, the idea of using this technique to help endangered species has aroused considerable interest. However, several issues limit this possibility, including the relatively low success rate at every stage of the cloning process, and the dearth of usable tissues from these rare animals. iPS cells have been produced from cells from a number of rare mammalian species and this is the method of choice for strategies to improve cloning efficiency and create new gametes by directed differentiation. Nevertheless information about other stem cell/progenitor capabilities of cells from endangered species could prove important for future conservation approaches and adds to the knowledge base about cellular material that can be extremely limited. Multipotent progenitor cells, termed skin-derived precursor (SKP) cells, can be isolated directly from mammalian skin dermis, and human cheek tissue has also been shown to be a good source of SKP-like cells. Recently we showed that structures identical to SKPs termed m-SKPs could be obtained from monolayer/ two dimensional (2D) skin fibroblast cultures. Here we aimed to isolate m-SKPs from cultured cells of three endangered species; giant panda (Ailuropoda melanoleuca); red panda (Ailurus fulgens); and Asiatic lion (Panthera leo persica). m-SKP-like spheres were formed from the giant panda buccal mucosa fibroblasts; whereas dermal fibroblast (DF) cells cultured from abdominal skin of the other two species were unable to generate spheres. Under specific differentiation culture conditions giant panda spheres expressed neural, Schwann, adipogenic and osteogenic cell markers. Furthermore, these buccal mucosa derived spheres were shown to maintain expression of SKP markers: nestin, versican, fibronectin, and P75 and switch on expression of the stem cell marker ABCG2. These results demonstrate that giant panda cheek skin can be a useful source of m-SKP multipotent progenitors. At present lack of sample numbers means that we can only postulate why we were unable to obtain m-SKPs from the lion and red panda cultures. However the giant panda observations point to the value of archiving cells from rare species, and the possibilities for later progenitor cell derivation.
Assuntos
Mucosa Bucal/citologia , Células-Tronco Multipotentes/fisiologia , Animais , Diferenciação Celular , Separação Celular , Células Cultivadas , UrsidaeRESUMO
Here, we explore the evolution and development of skin-associated adipose tissue with the goal of establishing nomenclature for this tissue. Underlying the reticular dermis, a thick layer of adipocytes exists that encases mature hair follicles in rodents and humans. The association of lipid-filled cells with the skin is found in many invertebrate and vertebrate species. Historically, this layer of adipocytes has been termed subcutaneous adipose, hypodermis and subcutis. Recent data have revealed a common precursor for dermal fibroblasts and intradermal adipocytes during development. Furthermore, the development of adipocytes in the skin is independent from that of subcutaneous adipose tissue development. Finally, the role of adipocytes has been shown to be relevant for epidermal homoeostasis during hair follicle regeneration and wound healing. Thus, we propose a refined nomenclature for the cells and adipose tissue underlying the reticular dermis as intradermal adipocytes and dermal white adipose tissue, respectively.
Assuntos
Tecido Adiposo Branco/anatomia & histologia , Derme/anatomia & histologia , Adipócitos Brancos/citologia , Adipócitos Brancos/fisiologia , Tecido Adiposo Branco/fisiologia , Animais , Derme/fisiologia , Folículo Piloso/anatomia & histologia , Folículo Piloso/fisiologia , Humanos , Camundongos , Regeneração/fisiologia , Especificidade da Espécie , Gordura Subcutânea/anatomia & histologia , Gordura Subcutânea/fisiologia , Terminologia como Assunto , Cicatrização/fisiologiaRESUMO
De novo organ regeneration has been observed in several lower organisms, as well as rodents; however, demonstrating these regenerative properties in human cells and tissues has been challenging. In the hair follicle, rodent hair follicle-derived dermal cells can interact with local epithelia and induce de novo hair follicles in a variety of hairless recipient skin sites. However, multiple attempts to recapitulate this process in humans using human dermal papilla cells in human skin have failed, suggesting that human dermal papilla cells lose key inductive properties upon culture. Here, we performed global gene expression analysis of human dermal papilla cells in culture and discovered very rapid and profound molecular signature changes linking their transition from a 3D to a 2D environment with early loss of their hair-inducing capacity. We demonstrate that the intact dermal papilla transcriptional signature can be partially restored by growth of papilla cells in 3D spheroid cultures. This signature change translates to a partial restoration of inductive capability, and we show that human dermal papilla cells, when grown as spheroids, are capable of inducing de novo hair follicles in human skin.
Assuntos
Microambiente Celular/fisiologia , Derme/citologia , Folículo Piloso/fisiologia , Regeneração/fisiologia , Esferoides Celulares/fisiologia , Técnicas de Cultura de Células , Biologia Computacional , Derme/fisiologia , Imunofluorescência , Perfilação da Expressão Gênica , Folículo Piloso/citologia , Humanos , Análise em Microsséries , Reação em Cadeia da Polimerase em Tempo Real , Biologia de SistemasRESUMO
The laboratory mouse is a key animal model for studies of adipose biology, metabolism and disease, yet the developmental changes that occur in tissues and cells that become the adipose layer in mouse skin have received little attention. Moreover, the terminology around this adipose body is often confusing, as frequently no distinction is made between adipose tissue within the skin, and so called subcutaneous fat. Here adipocyte development in mouse dorsal skin was investigated from before birth to the end of the first hair follicle growth cycle. Using Oil Red O staining, immunohistochemistry, quantitative RT-PCR and TUNEL staining we confirmed previous observations of a close spatio-temporal link between hair follicle development and the process of adipogenesis. However, unlike previous studies, we observed that the skin adipose layer was created from cells within the lower dermis. By day 16 of embryonic development (e16) the lower dermis was demarcated from the upper dermal layer, and commitment to adipogenesis in the lower dermis was signalled by expression of FABP4, a marker of adipocyte differentiation. In mature mice the skin adipose layer is separated from underlying subcutaneous adipose tissue by the panniculus carnosus. We observed that the skin adipose tissue did not combine or intermix with subcutaneous adipose tissue at any developmental time point. By transplanting skin isolated from e14.5 mice (prior to the start of adipogenesis), under the kidney capsule of adult mice, we showed that skin adipose tissue develops independently and without influence from subcutaneous depots. This study has reinforced the developmental link between hair follicles and skin adipocyte biology. We argue that because skin adipocytes develop from cells within the dermis and independently from subcutaneous adipose tissue, that it is accurately termed dermal adipose tissue and that, in laboratory mice at least, it represents a separate adipose depot.
Assuntos
Derme/embriologia , Derme/metabolismo , Proteínas de Ligação a Ácido Graxo/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Folículo Piloso/embriologia , Gordura Subcutânea/embriologia , Adipogenia , Adiposidade , Animais , Compostos Azo , Proteínas de Fluorescência Verde/metabolismo , Folículo Piloso/metabolismo , Imuno-Histoquímica , Lasers , Lipídeos/química , Masculino , Camundongos , Microscopia de Fluorescência , Gordura Subcutânea/metabolismo , Fatores de TempoRESUMO
The isolation of hair follicle dermal papilla cells has become an important technique in the field of cutaneous stem cell biology. These cells can be used for a number of biological and translational purposes. They are studied to identify the cellular characteristics and molecular factors that underpin the initiation, maintenance, and modulation of hair growth; to develop new human hair replacement techniques; and as a source of cells capable of being directed down a variety of different lineages. Here, we describe the isolation of hair follicle dermal papilla cells from both human and murine sources via the microdissection techniques used in our lab.
Assuntos
Técnicas de Cultura de Células/métodos , Folículo Piloso/citologia , Animais , Humanos , Camundongos , Células-Tronco/citologiaRESUMO
Traditional skin grafting techniques are effective but limited methods of skin replacement. Autologous transplantation of rapidly cultured keratinocytes is successful for epidermal regeneration, but the current gold-standard technique requires mouse fibroblast feeders and serum-rich media, with serum-free systems and dermal fibroblast (DF) feeders performing relatively poorly. Here, we investigated the capacity of human hair follicle dermal cells to act as alternative supports for keratinocyte growth. Dermal papilla (DP) dermal sheath (DS), DF and 3T3 cells were used as inactivated feeder cells for human keratinocyte coculture. Under conditions favouring dermal cells, proliferation of keratinocytes in the presence of either DS or DP cells was significantly enhanced compared with DF cells, at levels comparable to keratinocytes cultured under gold-standard conditions. Secreted protein acidic and rich in cysteine (SPARC) expression increased DS and DP cells relative to DFs; however, further experiments did not demonstrate a role in keratinocyte support.
Assuntos
Comunicação Celular/fisiologia , Proliferação de Células , Derme/citologia , Folículo Piloso/citologia , Queratinócitos/citologia , Células 3T3/citologia , Animais , Técnicas de Cocultura , Derme/metabolismo , Fibroblastos/citologia , Fibronectinas/metabolismo , Folículo Piloso/metabolismo , Humanos , Queratinócitos/metabolismo , Laminina/metabolismo , Camundongos , Osteonectina , Transplante de Pele/fisiologia , Proteínas Supressoras de Tumor/metabolismoRESUMO
Human multipotent skin derived precursor cells (SKPs) are traditionally sourced from dissociated dermal tissues; therefore, donor availability may become limiting. Here we demonstrate that both normal and diseased adult human dermal fibroblasts (DF) pre-cultured in conventional monolayers are capable of forming SKPs (termed m-SKPs). Moreover, we show that these m-SKPs can be passaged and that cryopreservation of original fibroblast monolayer cultures does not reduce m-SKP yield; however, extensive monolayer passaging does. Like SKPs generated from dissociated dermis, these m-SKPs expressed nestin, fibronectin and versican at the protein level. At the transcriptional level, m-SKPs derived from normal adult human DF, expressed neural crest stem cell markers such as p75NTR, embryonic stem cell markers such as Nanog and the mesenchymal stem cell marker Dermo-1. Furthermore, appropriate stimuli induced m-SKPs to differentiate down either mesenchymal or neural lineages resulting in lipid accumulation, calcification and S100ß or ß-III tubulin expression (with multiple processes). m-SKP yield was greater from neonatal foreskin cultures compared to those from adult DF cultures; however, the former showed a greater decrease in m-SKP forming capacity after extensive monolayer passaging. m-SKP yield was greater from adult DF cultures expressing more alpha-smooth muscle actin (αSMA). In turn, elevated αSMA expression correlated with cells originating from specimens isolated from biopsies containing more terminal hair follicles; however, αSMA expression was lost upon m-SKP formation. Others have shown that dissociated human hair follicle dermal papilla (DP) are a highly enriched source of SKPs. However, conversely and unexpectedly, monolayer cultured human hair follicle DP cells failed to form m-SKPs whereas those from the murine vibrissae follicles did. Collectively, these findings reveal the potential for using expanded DF cultures to produce SKPs, the heterogeneity of SKP forming potential of skin from distinct anatomical locations and ages, and question the progenitor status of human hair follicle DP cells.
Assuntos
Derme/citologia , Células-Tronco Multipotentes/citologia , Actinas/metabolismo , Adipogenia , Adulto , Biomarcadores/metabolismo , Células Cultivadas , Criopreservação , Derme/patologia , Feminino , Fibroblastos/citologia , Fibroblastos/patologia , Humanos , Proteínas de Filamentos Intermediários/metabolismo , Masculino , Pessoa de Meia-Idade , Células-Tronco Multipotentes/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Nestina , Neurônios/citologia , Osteogênese , Células de Schwann/citologia , Regulação para Cima , Versicanas/metabolismoRESUMO
In this study, we have demonstrated that cells of neural crest origin located in the dermal papilla (DP) exhibit endothelial marker expression and a functional activity. When grown in endothelial growth media, DP primary cultures upregulate expression of vascular endothelial growth factor receptor 1 (FLT1) mRNA and downregulate expression of the dermal stem cell marker α-smooth muscle actin. DP cells have demonstrated functional characteristics of endothelial cells, including the ability to form capillary-like structures on Matrigel, increase uptake of low-density lipoprotein and upregulate ICAM1 (CD54) in response to tumour necrosis factor alpha (TNF-α) stimulation. We confirmed that these observations were not due to contaminating endothelial cells, by using DP clones. We have also used the WNT1cre/ROSA26R and WNT1cre/YFP lineage-tracing mouse models to identify a population of neural crest-derived cells in DP cultures that express the endothelial marker PECAM (CD31); these cells also form capillary-like structures on Matrigel. Importantly, cells of neural crest origin that express markers of endothelial and mesenchymal lineages exist within the dermal sheath of the vibrissae follicle.
Assuntos
Diferenciação Celular , Linhagem da Célula , Derme/citologia , Células Endoteliais/citologia , Células-Tronco/citologia , Actinas/metabolismo , Animais , Biomarcadores/metabolismo , Diferenciação Celular/genética , Linhagem da Célula/genética , Células Clonais , Células Endoteliais/metabolismo , Endotélio Vascular/citologia , Endotélio Vascular/metabolismo , Citometria de Fluxo , Perfilação da Expressão Gênica , Células Endoteliais da Veia Umbilical Humana , Humanos , Integrases/metabolismo , Mesoderma/citologia , Mesoderma/metabolismo , Camundongos , Crista Neural/citologia , Crista Neural/metabolismo , Molécula-1 de Adesão Celular Endotelial a Plaquetas/metabolismo , Ratos , Células-Tronco/metabolismo , Fator de von Willebrand/metabolismoRESUMO
The underlying mechanism of immune privilege in hair follicle cell dermal papilla (DP) and sheath (DS) populations is not well understood, and the responsiveness of hair follicle dermal cells to pro-inflammatory challenge presently remains unknown. In this work, we describe acute NF-κB activation in human DS, DP and dermal fibroblast (DF) cells challenged with TNF-alpha and IL1-beta. In contrast, the DS and DP cells revealed an unexpected tolerance to bacterial LPS challenge relative to DF cells. Understanding follicle cell responses to typical pro-inflammatory stimuli is critical for diseases where collapse of hair follicle immune privilege is observed, and to further applications in autologous stem cell/wound healing therapeutics.
Assuntos
Derme/citologia , Células Epiteliais/metabolismo , Fibroblastos/metabolismo , Folículo Piloso/citologia , Lipopolissacarídeos/farmacologia , NF-kappa B/metabolismo , Pele/citologia , Transporte Ativo do Núcleo Celular/efeitos dos fármacos , Células Cultivadas , Células Epiteliais/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Humanos , Interleucina-1beta/farmacologia , Receptor 4 Toll-Like/metabolismo , Fator de Transcrição RelA/metabolismo , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
A recent series of papers, including Festa et al. (2011) in this issue, has revealed unexpected interdependent relationships among cell populations residing in and around the hair follicle. These interactions between different lineages of stem cells are crucial for hair follicle growth and cycling and point to a complex crosstalk in stem cell niches.
Assuntos
Adipócitos/citologia , Folículo Piloso/citologia , Pele/citologia , Células-Tronco/citologia , Animais , Feminino , Humanos , MasculinoRESUMO
Exogen is the process by which the hair follicle actively sheds its club fiber from the follicle. However, little is known about signals that govern the cellular mechanisms of shedding. Here, we have identified factors that are important in regulating either the retention or release of the hair club fiber from its epithelial silo within the follicle. Using the vibrissa follicle as our model, we isolated follicle segments containing club fibers and surrounding follicle tissue at different time points before their natural release from the hair follicle. We then performed microarray analysis to identify key molecular changes as the club fiber approached final release. Among the different classes of genes that were identified, we found changes in the expression pattern of protease inhibitors and proteases, suggesting that proteolysis may mediate fiber release, either through terminal differentiation or proteolytic cleavage. We validated transcriptional changes using reverse transcription-PCR, and further immunofluorescence analysis indicated that protease inhibitors surrounding the club fiber may have an important role in regulating the process of club fiber shedding. Our findings also highlighted that molecular differentiation of the innermost layer of cells immediately surrounding the club fiber, the companion(CL), is likely to be important in hair shedding.