Recent advances in simultaneous nitrification and denitrification for nitrogen and micropollutant removal: a review.

Simultaneous Nitrification and Denitrification (SND) is a promising process for biological nitrogen removal. Compared to conventional nitrogen removal processes, SND is cost-effective due to the decreased structural footprint and low oxygen and energy requirements. This critical review summarizes the current knowledge on SND related to fundamentals, mechanisms, and influence factors. The creation of stable aerobic and anoxic conditions within the flocs, as well as the optimization of dissolved oxygen (DO), are the most significant challenges in SND. Innovative reactor configurations coupled with diversified microbial communities have achieved significant carbon and nitrogen reduction from wastewater. In addition, the review also presents the recent advances in SND for removing micropollutants. The micropollutants are exposed to various enzymes due to the microaerobic and diverse redox conditions present in the SND system, which would eventually enhance biotransformation. This review presents SND as a potential biological treatment process for carbon, nitrogen, and micropollutant removal from wastewater.

Investigating the biodegradability of iodinated X-ray contrast media in simultaneous nitrification and denitrification system.

The present study investigated the biodegradation of three iodinated X-ray contrast media (ICM), namely, iopamidol, iohexol, and iopromide, in simultaneous nitrification-denitrification (SND) system maintained in a sequencing batch reactor (SBR). The results showed that variable aeration patterns (anoxic-aerobic-anoxic) and micro-aerobic condition were most effective in the biotransformation of ICM while achieving organic carbon and nitrogen removal. The highest removal efficiencies of iopamidol, iohexol, and iopromide were 48.24%, 47.75%, and 57.46%, respectively, in micro-aerobic condition. Iopamidol was highly resistant to biodegradation and possessed the lowest Kbio value, followed by iohexol and iopromide, regardless of operating conditions. The removal of iopamidol and iopromide was affected by the inhibition of nitrifiers. The transformation products after hydroxylation, dehydrogenation, and deiodination of ICM were detected in the treated effluent. Due to the addition of ICM, the abundance of denitrifier genera Rhodobacter and Unclassified Comamonadaceae increased, and the abundance of class TM7-3 decreased. The presence of ICM affected the microbial dynamics, and the diversity of microbes in SND resulted in improving the biodegradability of the compounds.

Anoxic-Aerobic-Anoxic sequencing batch reactor for enhanced nitrogen removal.

An anoxic-aerobic-anoxic process was established to achieve simultaneous removal of organic carbon and nitrogen from wastewater in a sequencing batch reactor. The optimum conditions were attained at a DO of 1.5 mg/L with 1 h pre-anoxic and post-anoxic periods. TOC, NH4+-N, and TN removal efficiencies were 98.76 ± 0.95 %, 98.52 ± 0.48 %, and 88.23 ± 0.62 %, respectively, at optimum conditions. Breakpoints in the pH, DO, and ORP curves provided a clear understanding of biochemical reactions happening in the reactor. Inhibition studies showed that 27.69 % of NH4+-N was removed through nitrogen removal pathways such as heterotrophic nitrification or direct conversion, and 20.55 % of TN was removed through aerobic denitrification. Microbial community analysis confirmed the presence of heterotrophic nitrifiers and aerobic denitrifiers. This study highlighted that the varied redox conditions offered by limited aeration would be beneficial for nitrogen removal, thereby reducing the energy usage and operating costs.