Role of glutamate ionotropic and benzodiazepine receptors in the ventromedial hypothalamic nucleus on anxiety.

The dorsomedial part of the ventromedial hypothalamic nuclei (VMHdm) has been related to the modulation of defensive behavior in mammals. The objective of the present study was to test the hypothesis that administration into the VMHdm of midazolam, a benzodiazepine receptor full agonist, or AP7, a glutamate NMDA receptor antagonist, would produce anxiolytic effects in the elevated plus-maze (EPM) or the Vogel's punished licking tests. Male Wistar rats with unilateral cannulae aimed at the VMHdm received intra-cerebral injections of midazolam (15-60 nmol/0.25 microL), AP7 (0.2-2 nmol/0.3 microL) or saline and were submitted to the behavioral tests. Midazolam (30 nmol) increased the percentage of time spent in open arms of the EPM. AP7, on the other hand, decreased open and enclosed arm exploration. In the Vogel test, however, both midazolam (30-60 nmol) and AP7 increased the number of punished licks. Histological control experiments found no significant effects when the drugs were injected into the nearby lateral hypothalamic area. These results suggest that facilitation of gabaergic or antagonism of glutamatergic neurotransmission in the VMHdm can produce anxiolytic-like effects.

Role of glutamate ionotropic receptors in the dorsomedial hypothalamic nucleus on anxiety and locomotor behavior.

The medial hypothalamus is proposed to play an important role in the modulation of defensive responses. Administration of a NMDA receptor antagonist (AP7) into the dorsomedial hypothalamic nucleus (DMH) of rats reduced exploratory behavior in the open field and elevated plus-maze (EPM), but failed to produce anxiolytic effects in the latter test. The objectives of the present work were to test the hypotheses that (i) AP7 injections into the DMH would also fail to induce anxiolytic effects in another model of anxiety, the Vogel's punished licking test; (ii) injection into the DMH of other glutamate ionotropic antagonists would also decreased exploratory behavior; and (iii) the decrease in exploratory activity found after AP7 administration into the DMH does not involve any gross locomotor impairment. Male Wistar rats (n=5-16/group) with cannulas aimed at the DMH were submitted to the following behavioral tests: EPM, Vogel, catalepsy and rota-rod. Diazepam (3 mg/kg) and haloperidol (2.5 mg/kg) were used as positive controls in the Vogel, rota-rod and catalepsy tests. AP7 failed to modify the number of punished licks in the Vogel test. It also did not induce any change on the rota-rod and catalepsy tests. Diazepam increased the number of punished licks and reduced the latency to fall in the rota-rod. Both 7-chlorokynurenic acid (4-8 nmol), an antagonist of the glycine competitive site in the NMDA receptor and 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo-[f]-quinoxaline-7-sulphonamide (NBQX, 1-10 nmol), a non-NMDA receptor antagonist, decreased the total distance moved in the EPM. The former compound also decreased open arm exploration at the dose of 4 nmol. The results suggest that the antagonism of ionotropic glutamate receptors in the DMH does not induce anxiolytic effects in the EPM or Vogel tests, but decreases exploratory behavior in a new environment.

[Dental fluorosis in children from Princesa Isabel, Paraiba]. / Fluorose dentária em crianças de Princesa Isabel, Paraíba.

Several communities in Paraíba have moderate or high levels of fluoride naturally present in the drinking water. A moderate prevalence of dental fluorosis (30-40%) has been observed in some areas where the levels of fluoride are regarded as "optimal" for the region (0.6 ppm). The aim of the present study was to determine the prevalence of dental fluorosis in Princesa Isabel, a city with "sub-optimal" fluoride levels (0.4 ppm). The sample comprised 142 schoolchildren (10- to 15-year-old subjects) randomly selected and examined by means of the TF (Thylstrup & Fejerskov) index. The clinical exams were carried out under indirect natural light by three calibrated examiners. Prior to the examination the teeth were cleaned and dried. Approximately 20% of the subjects examined presented with some degree of fluorosis. Seventy per cent were classified as TF 1 while 30% were classified as TF 2 to 5. The prevalence of fluorosis was higher in male subjects and in premolars. Although the observed prevalence of dental fluorosis was within the expected levels, other sources of systemic fluoride must be controlled. The observed prevalence of dental fluorosis is not a public health problem in this community.

GABAergic and glutamatergic modulation of exploratory behavior in the dorsomedial hypothalamus.

Systemic injection of glutamate NMDA receptor antagonists or drugs that facilitate GABA(A)-mediated neurotransmission produces anxiolytic effects. The dorsomedial hypothalamic (DMH) region is proposed to be a possible site of action of these drugs. The objective of the present study was to investigate if facilitation of GABA(A)-mediated neurotransmission or blockade of NMDA receptors in the DMH would produce anxiolytic effects in the elevated plus-maze (EPM). Seven days after surgery, male Wistar rats with unilateral cannulas in the DMH were submitted to the behavioral studies. Results showed that midazolam, a benzodiazepine anxiolytic (30-60 nmol/0.3 microl), produced a dose-dependent increase in open arm exploration without changing the number of enclosed arm entries, indicating an anxiolytic effect. This effect was antagonized by previous treatment with flumazenil, a benzodiazepine receptor antagonist (60 nmol/0.3 microl). Flumazenil alone had an anxiogenic effect, decreasing exploration of the open arms of the EPM. 2-Amino-7-phosphonoheptanoic acid (AP7), an NMDA receptor antagonist (0.2-2 nmol/0.3 microl), did not modify open arm exploration but decreased general exploratory activity. These results indicate that benzodiazepine receptors located in the DMH could modulate anxiety. Interference with NMDA receptor-mediated neurotransmission in this region, however, seems to change general exploratory activity rather than anxiety.

Cárie dentária no Brasil: alguns aspectos sociais, políticos e econômicos / Dental caries in Brazil: economics, politics and social aspects

Os autores abordam alguns aspectos sociais, políticos e econômicos da cárie dentária confrontando os avanços da VIII Conferência Nacional de Saúde e da Constituiçäo Federal de 1988 com a realidade da saude bucal no particular aspecto da cárie dentária

Evaluation of the elevated T-maze as an animal model of anxiety in the mouse.

The elevated T-maze has been developed as an animal model of anxiety to generate both conditioned and unconditioned fears in the same rat. This study explores a version of the elevated T-maze fit for mice. Inhibitory (passive) avoidance- conditioned fear-is measured by recording the latency to leave the enclosed arm during three consecutive trials. One-way escape- unconditioned fear-is measured by recording the time to withdraw from open arms. The results showed that mice do not appear to acquire inhibitory avoidance in the standard T-maze, since their latencies to leave enclosed arm did not increase along trials. Nevertheless, the open arms seemed to be aversive for mice, because the latency to leave the enclosed arm after the first trial was lower in a T-maze with the three enclosed arms than in the standard elevated T-maze. In agreement, the exposure of mice to an elevated T-maze without shield, that reduces the perception of openness, increased significantly the latencies to leave the enclosed arm. However, the absence of the shield also increased the time taken to leave the open arms when compared to that recorded in standard T-maze. Systematic observation of behavioral items in the enclosed arm has shown that risk assessment behavior decreases along trials while freezing increases. In the open arms, freezing did not appear to influence the high latency to leave this compartment, since mice spend only about 8% of their time exhibiting this behavior. These results suggest that mice acquire inhibitory avoidance of the open arms by decreasing and increasing time in risk assessment and freezing, respectively, along three consecutive trials. However, one-way escape could not be characterized. Therefore, there are important differences between mice (present results) and rats (previously reported results) in the performance of behavioral tasks in the elevated T-maze.