Granulomatous tracheitis and dermatitis due to Curvularia sp. in a horse: case report / [Traqueíte e dermatite granulomatosas associadas a Curvularia sp. em um equino: relato de caso]

Tracheal fungal infections in horses are rare. This case report describes surgical and clinical management of a filly with a Curvularia sp. infection within the trachea and skin that caused severe intraluminal granulomas and cutaneous nodules, respectively. The patient was successfully treated with itraconazole and surgical excision.(AU)

Infecções fúngicas traqueais em equinos são raras. Este relato de caso descreve condutas clínicas e cirúrgicas em uma égua com infecção por Curvularia sp. na traqueia e na pele, causando granulomas intraluminais severos e nódulos cutâneos, respectivamente. O animal foi tratado com sucesso com itraconazol e exérese cirúrgica.(AU)

Bloqueio do plexo braquial em um tamanduá-mirim (Tamandua tetradactyla) utilizando estimulador de nervos periféricos: relato de caso / Brachial plexus block in a tamanduá-mirim (Tamandua tetradactyla) using a peripheral nerve stimulator: Case report

Objetivou-se relatar a utilização do neurolocalizador para bloqueio do plexo braquial bilateral em tamanduá-mirim (Tamandua tetradactyla). O animal, pesando 5kg, atendido pelo Centro de Reabilitação de Animais Silvestres, foi encaminhado ao Hospital Veterinário da Universidade Federal de Mato Grosso do Sul para realização de exames complementares, sendo, posteriormente, encaminhado para cirurgia de osteossíntese de úmero e rádio/ulna esquerdo e colocação de fio de cerclagem em olécrano direito. O paciente foi pré-medicado com cetamina S (5mg/kg) + midazolam (0,15mg/kg), indução anestésica com propofol (5mg/kg) e manutenção anestésica com isoflurano, com o auxílio de máscara. Os parâmetros cardiovasculares e respiratórios foram monitorados durante todo o procedimento. Realizou-se o bloqueio do plexo braquial em ambos os membros utilizando-se estimulador de nervos periféricos. Os anestésicos locais empregados foram lidocaína 2% sem vasoconstritor (3mg/kg) + ropivacaína 0,75% sem vasoconstritor (1mg/kg). O bloqueio foi realizado primeiramente no membro torácico direito, e, após realização do procedimento cirúrgico, o mesmo bloqueio foi realizado no membro contralateral. O paciente teve recuperação tranquila ausente de vocalização e expressão álgica, e a soltura ocorreu após 120 dias.(AU)

The aim of this study was to report the use of the neurolocalizer for blocking the bilateral brachial plexus in tamanduá-mirin. The subject weighing 5kg was attended by the Center for the Rehabilitation of Wild Animals and referred to the Veterinary Hospital of the Federal University of Mato Grosso do Sul for complementary tests. Through the examinations, the need to perform humerus and left radius/ulna osteosynthesis and cerclagem placement on right olecranon was established. For surgery performance, the patient was pre-medicated with ketamine S (5mg/kg-1) associated to midazolam (0.15mg/kg-1) via intramuscular and intravenous propofol (5mg/kg-1) was used for induction. The anesthetic maintenance was performed with isoflurane, provided by an oxygen mask. Cardiovascular and respiratory parameters were monitored throughout the procedure. Brachial plexus block was performed in both limbs using peripheral nerve stimulator. The local anesthetics used were lidocaine without vasoconstrictor (3mg/kg-1) plus 0.75% ropivacaine without vasoconstrictor (1mg/kg-1). Blocking was first performed on the right thoracic limb followed by the surgical procedure. The same blockage was performed on the contralateral limb. The patient had a smooth recovery, without vocalization and pain. The release to its natural habitat occurred after 120 days.(AU)

Effects of intra-articular injection of mesenchymal stem cells associated with platelet-rich plasma in a rabbit model of osteoarthritis.

The current study aims to evaluate the macroscopic and histological effects of autologous mesenchymal stem cells (MSC) and platelet-rich plasma on knee articular cartilage regeneration in an experimental model of osteoarthritis. Twenty-four rabbits were randomly divided into four groups: control group, platelet-rich plasma group, autologous MSC undifferentiated group, and autologous MSC differentiated into chondrocyte group. Collagenase solution was used to induce osteoarthritis, and treatments were applied to each group at 6 weeks following osteoarthritis induction. After 60 days of therapy, the animals were euthanized and the articular surfaces were subjected to macroscopic and histological evaluations. The adipogenic, chondrogenic, and osteogenic differentiation potentials of MSCs were evaluated. Macroscopic and histological examinations revealed improved tissue repair in the MSC-treated groups. However, no difference was found between MSC-differentiated and undifferentiated chondrocytes. We found that MSCs derived from adipose tissue and platelet-rich plasma were associated with beneficial effects in articular cartilage regeneration during experimental osteoarthritis.

Comparison of analgesic and systemic effects of bupivacaine, methadone, or bupivacaine/methadone administered epidurally in conscious sheep.

OBJECTIVE: The aim of this study was to evaluate the combination of bupivacaine and methadone administered epidurally in sheep. METHODS: Six healthy female mixed-breed sheep weighing 35-46 kg and aged 12-18 months were included. Each sheep was assigned to receive three treatments: 0.5 mg/kg 0.25% bupivacaine (BP), 0.3 mg/kg 1% methadone (MT) or 0.25 mg/kg bupivacaine and 0.15 mg/kg methadone (BPMT). All drugs were injected into the lumbosacral space through an epidural catheter. Each animal received each treatment at random. Heart rate, arterial blood pressure (systolic, diastolic and mean), respiratory rate, rectal temperature, analgesia, sedation and motor block were determined before treatment and at predetermined intervals. RESULTS: The duration of analgesia was 240, 220, and 180 min for BP, MT and BPMT, respectively (P < 0.05). Motor block for all agents was mild to moderate. None or the treatments significantly altered the heart rate, blood pressure or respiratory rate. CONCLUSION: Our findings suggest that lumbosacral epidural administration of bupivacaine, methadone or a combination of the two drugs can provide perioperative analgesia in sheep as part of their management for surgical procedures in the flank and hindlimbs.

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay.

The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from adipose tissue. The analyzed and interpreted results suggest that genetic testing is needed to support clonal expansion safety in cell therapy procedures with MSCs. Furthermore, they also suggest that if the comet assay technique would be used as a genomic integrity screening assay, the protocol performed at pH = 12 (that yielded a frequency of damaged cells: tail intensity = 9.50 ± 0.60, tail moment = 0.0122 ± 0.0007; results are reported as means ± standard deviation) would be indicated as genomic damage, and that subsequent single-strand breaks occur at pH > 13 (frequency of damaged cells: tail intensity = 30.71 ± 4.23, tail moment = 0.0447 ± 0.0073). Our study demonstrates that, in the era of regenerative medicine, it is necessary to standardize and establish a battery of tests in order to identify genomic damage prior to MSC transplantation.