A Long-Term Study on Chemical Compounds and Their Location in Sweet Basil Leaves from Organic and Conventional Producers.

Bioactive compound profiles in organic and conventional sweet basil were analyzed by HPLC, and the enzymatic status and antioxidant status of plants cultivated with the two systems were also examined. Fluorescence microscopy was used for the determination of compounds' locations in the basil leaves. The experiment was conducted from 2019 to 2021. Organic and conventional basil samples were obtained directly from Polish herb producers. The results showed that the chemical profiles of organic and conventional basil leaves are different. Not only the cultivation method but also the experimental year had a significant impact on the antioxidant content in basil leaves. Organic basil contained significantly more dry matter (11.97 g 100 g-1 FW) compared to conventional one (10.54 g 100 g-1 FW) and a higher tendency for total phenolic compounds (5.24 mg g -1 DW) accumulation. The higher bioactive compound content reflects the antioxidant activity (61.0%, 54.33%, and 46%) in organic basil compared to conventional (46.87%, 38.055, and 39.24%) with respect to the analysis method (ABTS, DPPH, and FRAP). Catalase activity (39 µmol H2O2 min-1 mg-1) in organic basil was higher compared to conventional (23.19 µmol H2O2 min-1 mg-1) ones. The obtained results are very unique and could be used by herb producers as a key for high-quality basil production. The higher concentration of bioactive compounds in organic basil gives a better nutraceutical status to this popular herb.

Red Horse Chestnut and Horse Chestnut Flowers and Leaves: A Potential and Powerful Source of Polyphenols with High Antioxidant Capacity.

Aesculus flowers and leaves are an excellent source of bioactive compounds, including flavanols, phenolic acids, and anthocyanins, and the leaves also contain antioxidant carotenoids and chlorophylls. The aim of this study was to analyse and compare the amounts of bioactive compounds present in Aesculus hippocastanum and Aesculus × carnea flowers and leaves over two years. These two species from six independent locations (parks and green areas) located in Warsaw were assessed in this study. The dry matter by the scale method and polyphenol, carotenoid, and chlorophyll content by the HPLC method of the flowers and leaves was evaluated. Red horse chestnut flowers contained significantly more total carotenoids (40.6 µg/g FW) and chlorophylls (36.9 µg/g FW) than horse chestnut flowers, and red horse chestnut flowers contained higher levels of anthocyanins (5.41 µg/g FW) than other species. We observed that horse chestnut flowers were characterized by a higher total polyphenols concentration (9.45 µg/g FW) compared to red horse chestnut flowers. In addition, the analysis of leaves showed that all quality parameters were higher in red horse chestnut species. Five individual anthocyanins were identified in both species' flowers, but a higher concentration was found in red horse chestnut flowers, and pelargonidin-3-O-glucoside was the predominant form among a pool of total anthocyanins. In both experimental years, leaves (109.25 mMol/100 g FW and 112.0 mMol/100 g FW) were characterized by a higher antioxidant activity than flowers (27.0 mMol/100 g FW and 27.5 mMol/100 g FW).