Assuntos
Adenocarcinoma/imunologia , Antígenos de Neoplasias/imunologia , Antígenos de Superfície/imunologia , Colo/imunologia , Neoplasias Colorretais/imunologia , Glicoproteínas de Membrana/imunologia , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Antineoplásicos/imunologia , Especificidade de Anticorpos , Biomarcadores Tumorais/imunologia , Cromatografia em Gel , Epitélio/imunologia , Epitopos/imunologia , Fezes , Humanos , Mucosa Intestinal/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Peso Molecular , Neoplasias/imunologia , Especificidade de Órgãos , Células Tumorais Cultivadas/imunologiaRESUMO
A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.