Retrospective analysis of negative cytology results correlated with a positive high-risk in the human papillomavirus result and subsequent results of patients over a period of three years.

This research paper evaluates the efficacy of co-testing in precluding cervical cancer, with a particular focus on distinguishable outcomes of the human papillomavirus (HPV) vs. cytology tests. A retrospective review of 5948 patients, who tested positive for high-risk HPV but showed negative cytologic findings, revealed that 15.006% tested positive in subsequent screenings. A comparative analysis of various commercial HPV tests highlighted the precision of mRNA-based HPV testing by Aptima (Hologic) in reducing the likelihood of false-negative cytology. The paper challenges the conviction that a negative cytology alone suffices advocating for a condensed testing interval in instances of positive HPV outcomes, thereby facilitating earlier intervention and optimal preventive care. These findings unveil an exigency for reconsidering preventive strategies based on test outcomes.

Organization of the 43rd European Congress of Cytology in the SARS-CoV-2 pandemic period: A report.

The 43rd European Congress of Cytology in Wroclaw, Poland, was held as a hybrid meeting in the Fall of 2021. After nearly 2 years without in-person cytology conferences, the 43rd Congress represents 1 of the first major international scientific meetings to occur during the severe acute respiratory syndrome-coronavirus 2 pandemic. Since March 2020, the pandemic situation substantially modified the organization of scientific meetings because of both domestic and international travel restrictions, new health standards, and concern among participants, resulting in new alternative forms of virtual conferencing. Cancer (Cancer Cytopathol) 2022;130:000-000.

ARID1A/BAF250a is significantly overexpressed in primary invasive breast cancer.

BACKGROUND: ARID1A (also known as BAF250a, p270 or SMARCF1) is a major component of the mammalian SWI/SNF family that is involved in the regulation of the chromatin structure. ARID1A gene mutations have been associated with many types of malignancies, including breast cancer. This study aimed to explore the expression of BAF250a protein in breast cancer and its association with the clinical and pathological characteristics and prognosis of breast cancer. METHODS: We assessed the BAF250a expression in 119 invasive breast carcinomas samples and 92 healthy control and correlated this expression pattern with various clinical and pathologic parameters including histologic type and grade, tumor size, lymph node status, estrogen receptor (ER) status, progesterone receptor (PR) status. Immunohistochemical analysis of BAF250a, ER, PR, was carried out, and evaluation of stainings was performed. RESULTS: The mean value of BAF250a expression in the experimental group was higher than in healthy control (P=0.001). The expression is unrelated to age, menopausal status, lymph node status, tumor size and location, grade and histologic type of tumor, and hormonal status (ER, PR). CONCLUSIONS: These data suggest that BAF250a is overexpressed in breast cancers. BAF250a may play context-dependent tumor-promoting and tumor-suppressive roles in cancer.

Differentiation of the Strength of Back Muscle Contraction Under Fatigue: Does Force Feedback Play a Role?

OBJECTIVE: This study investigates the ability of subjects to differentiate the strength of back muscle contraction with and without feedback information on force produced under fatigue and nonfatigue conditions. DESIGN: Controlled laboratory study. SETTING: Research laboratory environment. PARTICIPANTS: A group of 52 healthy young men participated in the study. INTERVENTION: Subjects self-estimated 50% of the maximal voluntary isometric contraction of back muscles either on their own volition or on the basis of information about the actual force, before and after the Sørensen fatigue test. MAIN OUTCOME MEASURES: The force was measured by means of the FiTRO Back Dynamometer. RESULTS: The self-estimated 50% maximal voluntary isometric contraction was significantly higher than the one calculated from maximal voluntary isometric contraction during 10 trials in 2 repeated sessions (8.3% and 10.0%, P < .05). However, when feedback on the force produced was provided, significantly higher values were observed during an initial trial in both sessions (8.5%, P = .04 and 12.1%, P = .01). Subjects were able to estimate the target force during the following trials. Fatigue induced a decrease in peak force (7.7%, P = .04), whereas the ability to regulate the prescribed force was not compromised. Constant error was lower with than without force feedback during both measurements (2.15% and 6.85%; 3.06% and 8.56%). However, constant and variable errors were greater under fatigue than nonfatigue conditions (8.43% and 5.55%; 0.41% and 0.37%). Similarly, root mean square error decreased with force feedback (from 6.88% to 3.48% and from 8.74% to 5.09%) and increased under fatigue (from 5.87% to 8.67%). CONCLUSIONS: These findings indicate that force feedback plays a role in the differentiation of the strength of back muscle contraction, regardless of fatigue. It contributes to a more precise regulation of force produced during voluntary isometric contraction of back muscles. This promising method awaits further experimentation to be applied for individuals with low back pain.

Comparison of the ICC location in the gallbladder wall in patients with cholelithiasis and patients with non-calculous changes.

Interstitial cells of Cajal (ICC) were first described by Santiago Ramon y Cajal over 100 years ago. They are thought to play an important role in the regulation of gastrointestinal motility. There is increasing evidence that the decline in their number in the gallbladder wall contributes to the formation of concrements. The aim of the study was to determine the exact location of interstitial cells of Cajal in the gallbladder wall in patients with calculous and non-calculous cholecystitis. Sixty-eight patients were examined, of whom 50 were cases of cholelithiasis and 18 were of non-calculous cholecystitis. The technique of immunohistochemistry with the CD117 antibody was used to determine the cells of Cajal, while to distinguish them from mast cells the technique with mast cell tryptase (MCT) was applied. Redistribution of the interstitial cells of Cajal from the muscle membrane to lamina propria of mucous tissue was observed in the cases of cholelithiasis, while in the group of non-calculous cholecystitis most of the ICC was located within the muscle tissue.

Between-side differences in trunk rotational power in athletes trained in asymmetric sports.

BACKGROUND: The asymmetric loading of trunk muscles in sports like golf or tennis may cause side-to-side imbalances in rotational muscle strength and endurance. Such imbalances may be compounded by the presence of low back pain (LBP) and related injuries. However, trunk rotational power is a better predictor of athlete performance, and therefore its ability to reveal these asymmetries/dysbalances should be investigated. OBJECTIVE: This study compares peak and mean values of power during trunk rotations on the dominant and non-dominant side in golfers, ice-hockey players, tennis players, and an age-matched control group of fit individuals. METHODS: Groups of 17 golfers, 17 ice-hockey players, 21 tennis players, and 39 fit individuals performed standing trunk rotations to each side with a bar weight of 5.5, 10.5, 15.5, and 20 kg placed on the shoulders. Peak power and mean power in the acceleration phase of trunk rotations were measured using the FiTRO Torso Premium system. RESULTS: Peak power and mean power in the acceleration phase of trunk rotations were significantly higher on the dominant (D) than non-dominant (ND) side at weights of 5.5 kg (14 and 14%), 10.5 kg (17 and 14%), 15.5 kg (16 and 15%), and 20 kg (16 and 16%) in ice-hockey players, at 5.5 kg (14 and 13%), 10.5 kg (17 and 14%), and 15.5 kg (15% - only peak power) in tennis players, and at 5.5 kg (17 and 18%) and 10.5 kg (19 and 17%) in golfers. However, their values did not differ significantly at these weights (< 10%) in the age-matched control group. The D/ND ratio was the highest in ice-hockey players (1.18, 1.19), followed by golfers (1.16, 1.17) and finally tennis players (1.12, 1.16). CONCLUSION: Taking into account significantly higher trunk rotational power on the dominant than the non-dominant side in golfers, tennis players and ice-hockey players at lower and/or higher weights and no significant side-to-side differences in a control group of fit individuals, this parameter may be considered specific to their asymmetric loading during trunk rotations. However, whether these asymmetries/dysbalances expressed by the D/ND ratio could also identify the likelihood of LBP, needs to be proven.

Bridging neighboring civilizations at academic grounds: a story of Zygmunt Albert - Rector illustrissimus ac magnificus Academiae Medicae Wratislaviensis - a pathologist who visualized gamma-glutamyl transpeptidase.

The life stories can constitute more than simple biographies to remain great lessons of honesty, grit and steadfastness in keeping standards of medical science within a strong moral fiber and flexible wiseness in hard terms like in case of Zygmunt Albert (1908-2001). This eminent pathologist histochemically visualized tissue distribution of gamma-glutamyl transpeptidase in liver and other organs under various conditions. He was also deeply involved in experimental pathology of liver, particularly in his comprehensive studies on chrysoidin-induced hepatoma that should bear eponymic name Albert's hepatoma. As he had both German and Polish roots, he became an eminent personage that wisely and consequently bridged neighboring civilizations in hard terms of escalation of hate in prewar times, during World War II and in postwar period. After he meticulously recorded Nazi crimes in Lvov, he appealed for justice in case of Nazi massive murders of Lvov Professors. He obtained his Associate Professorship in Anatomical Pathology in Lvov (Lemberg) and was one of rebuilders of Medical Faculty in postwar Wroclaw (Breslau) to serve as the first Rector Magnificus of Medical Academy of Wroclaw.

Association of Trunk Rotational Velocity with Spine Mobility and Curvatures in Para Table Tennis Players.

This study investigates the relationship of trunk rotational velocity with mobility and curvature of the spine in wheelchair table tennis players. Eleven para table tennis players and 13 able-bodied athletes performed 5 seated trunk rotations to each side with 1 kg barbell placed on the shoulders. Trunk rotational velocity and respective angular displacement were significantly lower in para table tennis players compared to able-bodied athletes. Both groups showed similar values of thoracic kyphosis. However, para table tennis players exhibited lower lumbar inversion and pelvic retroversion compared to able-bodied athletes. Peak and mean velocity in the acceleration phase of trunk rotation correlated with angular displacement in both para table tennis players (r=0.912, p=0.001; r=0.819, p=0.013) and able-bodied athletes (r=0.790, p=0.026; r=0.673, p=0.032). These velocity values were also associated with lumbar curvature (r=-0.787, p=0.003; r=- 0.713, p=0.009) and pelvic tilt angle (r=0.694, p=0.014; r=0.746, p=0.007) in para table tennis players. Findings indicate that slower velocity of trunk rotations in para table tennis players might be due to their limited range of trunk rotational motion. Decreased posterior concavity could also contribute to these lower values. However, other biomechanical factors may have an impact on the association between these variables and have yet to be documented.

Anaplastic thyroid carcinoma with rapid thyrotoxicosis - a case report and the literature review.

INTRODUCTION: Anaplastic thyroid carcinoma (ATC) is one of the most aggressive human malignancies and constitutes approximately 1.6-5% of the malignant neoplasms of the thyroid gland. ATC usually manifests itself with the local symptoms due to a rapidly enlarging thyroid mass, and as other thyroid cancers, has only seldom been reported to cause thyrotoxicosis. Up to now only 9 cases of ATC with concomitant thyrotoxicosis have been described. CASE REPORT: We report a rare case of a 66-year-old woman, who had had the preexisting large, euthyroid multinodular goiter for almost 50 years. She was consulted by a doctor because of a 4-week history of thyrotoxicosis, symptoms of the congestive heart failure and a rapid increase in the size of the goiter. Thyroid hormone levels were consistent with a hyperthyroid state. The fine-needle aspiration biopsy confirmed a diagnosis of the anaplastic thyroid carcinoma, the small cells variant. The 99m Tc-pertechnetate scintigraphy visualized non-homogenous tracer distribution with hot nodules. She was given a doxorubicin (20 mg/week) and required the continuous antithyroid treatment. The patient died a one year after the first symptoms of the disease occurred. DISCUSSION: The association between ATC and a thyrotoxic state is very rare. In most cases, thyrotoxicosis concomitant with ATC was thought to be a result of the destruction of the thyroid follicles by the rapid infiltration with malignant cells, resulting in the leakage of preformed hormones to the circulation. In that case the most probable cause of thyrotoxicosis was the multinodular goiter coexisting with ATC. A simultaneous onset of tumor growth, thyrotoxicosis and a relatively long survival time of our patient is worth to notice and discuss.

Clinical validation of nuclear factor kappa B expression in invasive breast cancer.

Breast cancer is the most commonly diagnosed cancer in Polish women. The expression of transcription nuclear factor kappa B, a key inducer of inflammatory response promoting carcinogenesis and cancer progression in breast cancer, is not well-established. We assessed the nuclear factor kappa B expression in a total of 119 invasive breast carcinomas and 25 healthy control samples and correlated this expression pattern with several clinical and pathologic parameters including histologic type and grade, tumor size, lymph node status, estrogen receptor status, and progesterone receptor status. The data used for the analysis were derived from medical records. An immunohistochemical analysis of nuclear factor kappa B, estrogen receptor, and progesterone receptor was carried out and evaluation of stainings was performed. The expression of nuclear factor kappa B was significantly higher than that in the corresponding healthy control samples. No statistical difference was demonstrated in nuclear factor kappa B expression in relation to age, menopausal status, lymph node status, tumor size and location, grade and histologic type of tumor, and hormonal status (estrogen receptor and progesterone receptor). Nuclear factor kappa B is significantly overexpressed in invasive breast cancer tissues. Although nuclear factor kappa B status does not correlate with clinicopathological findings, it might provide important additional information on prognosis and become a promising object for targeted therapy.

The Association Between HSP90/topoisomerase I Immunophenotype and the Clinical Features of Colorectal Cancers in Respect to KRAS Gene Status.

AIM: The aim of this study was to investigate heat shock protein 90 (HSP90) and topoisomerase I (Topo I) expression and the association between both proteins and clinicopathological parameters of colorectal cancer (CRC), in order to describe their role in tumor biology regarding to Kirsten Ras (KRAS) - positive/negative cases. MATERIALS AND METHODS: Expression of HSP90 and Topo I, and KRAS gene mutations were estimated in primary CRCs. RESULTS: HSP90/Topo I immunophenotype correlated with gender, Duke staging, tumor grade and lymph node metastasis (p<0.01). Positive correlation was found between KRAS mutation and HSP90 expression (p=0.02). HSP90, Topo I expression, and co-expression of HSP90/Topo I correlated with unfavorable parameters of CRCs in respect to KRAS gene status (p<0.001). CONCLUSION: Our results revealed that cooperation between HSP90 and Topo I expression exists in CRCs, independently of KRAS gene status, suggesting that co-expression of both proteins might be considered as a double target on individual tumor cells.

Bethesda System in the evaluation of thyroid nodules: Review.

Fine needle aspiration (FNA) biopsy is an important and widely accepted method used in the diagnosis of patients with thyroid nodules.The lack of consistency in reporting FNA taken from the thyroid gland has led to divergences in the calculation of the sensitivity and specificity of the method. Discrepancies depend on what was considered to be true or false negative/positive. This resulted in confusion among doctors, who had to decide how to manage the patient with constantly changing positive or negative results of the FNA. The problem was solved in 2007, when "The Bethesda System for Reporting Thyroid Cytopathology" (TBSRTC) was introduced. Generally, TBSRTC, compared to previous systems, results in reducing the number of non-diagnostic/indeterminate cases and enables a better clinical usefulness of the results of the FNA of the thyroid gland. This is probably due to the introduction of more standard criteria for interpreting and reporting. TBSRTC improves communication between cytopathologists, reduces the number of unnecessary operations on benign lesions, and makes it possible to perform the operation on time in patients with malignant lesions and predict the risk of thyroid cancer. It provides a simple and reliable exchange of data not only between various laboratories but also between institutions throughout the world. Research shows that all indicators of malignancy calculated for all categories have similar value to the recommended rate of malignancy.

Three months of resistance training in overweight and obese individuals improves reactive balance control under unstable conditions.

BACKGROUND: Contrary to static and dynamic balance, there is a lack of scientific evidence on the training induced changes in reactive balance control in response to unexpected perturbations in overweight and obese individuals. OBJECTIVE: This study evaluates the effect of 3 months of resistance and aerobic training programs on postural responses to unexpected perturbations under stable and unstable conditions in the overweight and obese. METHODS: A group of 17 overweight and obese subjects, divided into two groups, underwent either resistance or aerobic training for a period of 3 months (3 sessions per week). Prior to and after completing the training, they performed the load release balance test while standing on either a stable or unstable surface, with eyes open and closed. RESULTS: Peak posterior center of pressure (CoP) displacement, and the time to peak posterior CoP displacement during a bipedal stance on a foam surface with eyes open (17.3%, p = 0.019 and 15.4%, p = 0.029) and eyes closed (15.0%, p = 0.027 and 13.2%, p = 0.034), decreased significantly. In addition, the total anterior to posterior CoP displacement, and the time from peak anterior to peak posterior CoP displacement, both with eyes open (18.1%, p = 0.017 and 12.2%, p = 0.040) and eyes closed (16.3%, p = 0.023 and 11.7%, p = 0.044), also significantly decreased. However, after completing the resistance training, the parameters registered while standing on a stable platform, both with eyes open and closed, did not change significantly. The group that underwent an aerobic training also failed to show any significant changes in parameters of the load release balance test. CONCLUSION: Three months of resistance training in overweight and obese subjects improves reactive balance control in response to unexpected perturbations under unstable conditions, both with and without visual cues. Due to the fact that this unstable load release balance test was found to be sensitive in revealing post-training changes, it would be suitable for implementing in the functional diagnostic for this group, in addition to complementing existing testing methods.

Muscular Power during a Lifting Task Increases after Three Months of Resistance Training in Overweight and Obese Individuals.

BACKGROUND: This study evaluates the effect on power produced during a modified lifting task in the overweight and obese after three months of either resistance or aerobic training. METHODS: Seventeen male subjects divided randomly into two groups performed deadlift and deadlift high pull, both with increasing weights up to maximal power, prior to and after the training programs (three sessions per week). RESULTS: Their mean power increased significantly during the deadlift at 20 kg (14.3%, p = 0.026), 30 kg (17.7%, p = 0.008), 40 kg (16.5%, p = 0.011), 50 kg (14.5%, p = 0.020), and 60 kg (14.3%, p = 0.021) and during the deadlift high pull at 30 kg (9.9%, p = 0.037), 40 kg (10.1%, p = 0.035), and 50 kg (8.2%, p = 0.044) after the resistance training. However, the group that participated in the aerobic training failed to show any significant changes in power performance during either the deadlift or deadlift high pull. CONCLUSION: Three months of resistance training enhances power outputs during a lifting task with weights from 30 to 50 kg (~40%⁻60% of 1-repetition maximum) in the overweight and obese. Because this test was sensitive in revealing pre-post training changes in lifting performance, it should be implemented in the functional diagnostics for overweight and obese individuals and also complement existing testing methods.

Unilateral Stability and Visual Feedback Body Control Improves After Three-Month Resistance Training in Overweight Individuals.

The authors evaluated the effect of 3 months of resistance and aerobic training (3 sessions/week) on body balance in a group of 25 overweight and obese individuals. Prior to and after the training, they performed static and task-oriented balance tests under various conditions. Mean center of pressure (CoP) velocity and mean trace length of the CoP in the y-axis registered during a one-legged stance significantly decreased after the resistance training (19.1%, p = .024; 29.3%, p = .009). Mean trace length of the CoP in the y-axis decreased significantly also during a bipedal stance on a foam surface with eyes open and closed (10.9%, p = .040; 18.2%, p = .027). In addition, mean CoP distance and mean squared CoP distance in the anteroposterior direction during a visually guided center of mass (CoM) tracking task significantly improved (14.7%, p = .033; 28.2%, p = .016). However, only mean trace length of the CoP in the y-axis during a bipedal stance on a foam surface with eyes open and closed significantly decreased after the aerobic training (10.3%, p = .047; 16.5%, p = .029). It may be concluded that resistance training is more efficient for the improvement of the anteroposterior unilateral stability and the accuracy of the regulation of the CoM anteroposterior position than aerobic training in overweight and obese individuals.

Upper and Lower Body Muscle Power Increases After 3-Month Resistance Training in Overweight and Obese Men.

This study evaluates the effect of 3 months resistance and aerobic training on muscle strength and power in 17 male overweight and obese men. Subjects underwent either a resistance or aerobic training for a period of 3 months (three sessions per week). Peak isometric force, rate of force development, peak power and height of countermovement and squat jumps, reactive strength index, and mean power in the concentric phase of bench presses were all assessed prior to and after completing the training program. Results identified a significant increase of mean power during both countermovement bench presses at 30 kg (18.6%, p = .021), 40 kg (14.6%, p = .033), and 50 kg (13.1%, p = .042) and concentric-only bench presses at 30 kg (19.6%, p = .017) and 40 kg (13.9%, p = .037) after the resistance training. There was also a significant increase in the height of the jump (12.8%, p = .013), peak power (10.1%, p = .026), and peak velocity (9.7%, p = .037) during the countermovement jump and height of the jump (11.8%, p = .019), peak power (9.6%, p = .032), and peak velocity (9.5%, p = .040) during the squat jump. There were no significant changes in the reactive strength index, peak force, and the rate of force development after the resistance training. The aerobic group failed to show any significant improvements in these parameters. It may be concluded that 3 months of resistance training without caloric restriction enhances upper and lower body muscle power in overweight and obese men.

Balance Performance During Perturbed Standing Is Not Associated With Muscle Strength and Power in Young Adults.

The authors investigate the ways in which varied postural responses to translating platform perturbations are associated with the variables of strength and power. Twenty-four physically active and 27 sedentary young adults were exposed to a set of postural perturbations at varied velocities (10 and 20 cm/s) and the respective accelerations (6.4 and 6.9 m/s2), constant distance (6 cm), and 4 directions of platform motion (forward, backward, left-lateral, and right-lateral). They also performed maximum voluntary isometric contraction (MVC) and chair rising/chair jumping tests. The analysis of variance revealed significant interaction effect for peak center of pressure displacement, direction by velocity: F3,129 = 24.43, p = .002; and direction by acceleration: F3,129 = 34.18, p = .001. There were no significant correlations between peak center of pressure displacements and peak force and peak rate of force development measured during MVC in either standing (r = .27-57) or sitting positions (r = .12-51) and peak power during chair jumping (r = .47-.59) in all participants. As such, only a small proportion of variance was explained (9-39%, 3-23%, and 23-41%, respectively). In conclusion, interaction effects indicate that the composition of stimuli strongly influences compensatory responses and this effect is more pronounced in sedentary than in physically active young adults. Nevertheless, the dynamic balance is not associated with muscle strength and power in either group.

Jumping From a Chair is a More Sensitive Measure of Power Performance In Older Adults Than Chair Rising.

Background/Study Context: The study estimates the reliability of peak velocity and peak power during chair rising and chair jumping tests and their ability to discriminate between different age and physical activity level groups. METHODS: Physically active and sedentary individuals (N = 262) of different ages (young: 22.9 ± 2.0 years, range: 21-25 years; older: 63.1 ± 1.8 years, range: 61-65 years) performed, in random order, chair rising and chair jumping tests on a force plate. Randomly selected young subjects performed both tests repeatedly on two different occasions separated by 1 week. From the sitting position with the arms crossed on the chest, they either stand up completely (chair rising test), or jump as high as possible (chair jumping test). RESULTS: The test-retest reliability of peak power and peak velocity during chair rising as well as chair jumping was excellent, with high intraclass correlation coefficients (ICCs; .90-.98) and low standard error of measurement (SEM; 7.0-9.1%). Post hoc analysis revealed significant differences in peak power and peak velocity between the sedentary and physically active young and older subjects. However, greater coefficients of variation for both parameters were found for chair jumping than chair rising (21.1-40.2% vs. 11.0-15.2%). Additionaly, there were moderate correlations of peak power and peak velocity between chair rising and chair jumping (r = .42-.49). There were greater within- and between-group differences in peak force and peak power and a steeper increase in their values during the initial phase of chair jumping than chair rising. CONCLUSION: Both chair rising and chair jumping tests provide reliable data and are valid indicators of lower body power in young and older adults. However, jumping from a chair is a more sensitive measure of strength and power performance than chair rising.