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J Biophotonics ; 11(3)2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-29215208

RESUMO

In this study, we introduce the novel image-guided recording system (IGRS) for efficient interpretation of neuronal activities in the brain slice. IGRS is designed to combine microelectrode array (MEA) and optical coherence tomography at the customized upright microscope. It allows to record multi-site neuronal signals and image of the volumetric brain anatomy in a single body configuration. For convenient interconnection between a brain image and neuronal signals, we developed the automatic mapping protocol that enables us to project acquired neuronal signals on a brain image. To evaluate the performance of IGRS, hippocampal signals of the brain slice were monitored, and corresponding with two-dimensional neuronal maps were successfully reconstructed. Our results indicated that IGRS and mapping protocol can provide the intuitive information regarding long-term and multi-sites neuronal signals. In particular, the temporal and spatial mapping capability of neuronal signals would be a very promising tool to observe and analyze the massive neuronal activity and connectivity in MEA-based electrophysiological studies.


Assuntos
Encéfalo/citologia , Encéfalo/diagnóstico por imagem , Eletrofisiologia/instrumentação , Neurônios/citologia , Tomografia de Coerência Óptica , Animais , Encéfalo/fisiologia , Espaço Extracelular/metabolismo , Processamento de Imagem Assistida por Computador , Camundongos , Camundongos Endogâmicos C57BL , Microeletrodos , Análise Espaço-Temporal
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