RESUMO
Tobacco, a crop of significant economic importance, was greatly influenced in leaf quality by protein content. However, current processing parameters fail to adequately meet the requirements for protein degradation. Microorganisms possess potential advantages for degrading proteins and enhancing the quality of tobacco leaves, and hold substantial potential in the process of curing. To effectively reduce the protein content in tobacco leaves, thereby improving the quality and safety of the tobacco leaves. In this study, tobacco leaf were used as experimental material. From these, the BSP1 strain capable of effectively degrading proteins was isolated and identified as Bacillus subtilis by 16S rDNA analysis. Furthermore, the mechanisms were analyzed by integrating microbiome, transcriptome, and metabolome. Before curing, BSP1 was applied to the surface of tobacco leaves. The results indicated that BSP1 effectively improves the activity of key enzymes and the content of related substances, thereby enhancing protein degradation. Additionally, protein degradation was achieved by regulating the diversity of the microbial community on the surface of the tobacco leaves and the ubiquitin-proteasome pathway. This study provided new strategies for extracting and utilizing functional strains from tobacco leaves, opening new avenues for enhancing the quality of tobacco leaves.
RESUMO
Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations on the surface of tobacco leaves and in the rhizosphere soil. A strain capable of starch degradation, designated as BS3, was successfully isolated and identified as Bacillus subtilis by phylogenetic tree analysis based on 16SrDNA sequence. The application of BS3 on tobacco significantly enhanced enzyme activity and accelerated starch degradation during the curing process. Furthermore, analyses of the metagenome, transcriptome, and metabolome indicated that the BS3 strain facilitated starch degradation by regulating surface microbiota composition and affecting genes related to starch hydrolyzed protein and key metabolites in tobacco leaves. This study offered new strategies for efficiently improving the quality of tobacco leaves.
RESUMO
Underground construction projects such as tunnel construction are at high risk of water-induced disasters. Because this type of disaster poses a serious threat to worker safety and productivity, instruments and methods that can accurately detect the water source are critical. In this study, a water detection instrument that combines Magnetic Resonance Sounding (MRS) and Time-domain Electromagnetic Method (TEM) techniques to yield a joint MRS-TEM interpretation method was developed for narrow underground spaces such as tunnels. Joint modules including a transmitter and receiver were developed based on a dual-purpose and modular design concept to minimize the size and weight of the instrument and consequently facilitate transportation and measurement. Additionally, wireless control and communication technology was implemented to enable inter-module cooperation and simplify instrument wiring, and wireless synchronization was accomplished by implementing a Global Positioning System (GPS)-based timing scheme. The effectiveness and reliability of the instrument were verified via indoor laboratory tests and field measurement signal tests. Furthermore, the practicability of the combined instrument and its interpretation method was verified via a field case performed in a tunnel in Hubei, China.
RESUMO
Magnetic resonance sounding (MRS) is a novel geophysical method to detect groundwater directly. By applying this method to underground projects in mines and tunnels, warning information can be provided on water bodies that are hidden in front prior to excavation and thus reduce the risk of casualties and accidents. However, unlike its application to ground surfaces, the application of MRS to underground environments is constrained by the narrow space, quite weak MRS signal, and complex electromagnetic interferences with high intensities in mines. Focusing on the special requirements of underground MRS (UMRS) detection, this study proposes the use of an antenna with different turn numbers, which employs a separated transmitter and receiver. We designed a stationary coil with stable performance parameters and with a side length of 2 m, a matching circuit based on a Q-switch and a multi-stage broad/narrowband mixed filter that can cancel out most electromagnetic noise. In addition, noises in the pass-band are further eliminated by adopting statistical criteria and harmonic modeling and stacking, all of which together allow weak UMRS signals to be reliably detected. Finally, we conducted a field case study of the UMRS measurement in the Wujiagou Mine in Shanxi Province, China, with known water bodies. Our results show that the method proposed in this study can be used to obtain UMRS signals in narrow mine environments, and the inverted hydrological information generally agrees with the actual situation. Thus, we conclude that the UMRS method proposed in this study can be used for predicting hazardous water bodies at a distance of 7-9 m in front of the wall for underground mining projects.
RESUMO
Due to its unique sensitivity to hydrogen protons, magnetic resonance sounding (MRS) is the only geophysical method that directly detects water and can provide nondestructive information on subsurface aquifer properties. The relationship between the surface MRS signal and the location and characteristics of aquifers using large-coil (typically 50-150 m) sensors has been discussed based on forward modelling and experiments. However, few researchers have studied underground MRS using a small-coil sensor. In this paper, a parametric study and a large-scale physical model test were conducted to shed light on the critical response characteristics of underground MRS using a small-coil sensor. The effects of the size and number of turns of the transmitter coil and receiver coil, the geomagnetic declination, the geomagnetic inclination, and the position, thickness, and water content of a water-bearing structure on the performance of the underground MRS were studied based on numerical simulations. Furthermore, we derived the kernel function and underground MRS signal curves for a water-bearing structure model based on the simulations. Finally, a large-scale physical model test on underground MRS using a small-coil sensor was performed using a physical test system for geological prediction of tunnels at Shandong University. The results show that the inversion results of the physical model test were in good agreement with the physical prototype results. Using both numerical modeling and physical model tests, this study showed that underground MRS using a small-coil sensor can be used to predict water-bearing structures in underground engineering.
RESUMO
Magnetic resonance sounding (MRS) using the Earth's magnetic field is a noninvasive and on-site geophysical technique providing quantitative characteristics of aquifers in the subsurface. When the MRS technology is applied in a mine or tunnel for advance detecting the source of water that may cause disastrous accident, spatial constraints limit the size of coil sensor and thus lower the detection capability. In this paper, a coil sensor for detecting the weak MRS signal is designed and the signal to noise (SNR) for the coil sensor is analyzed and optimized. The coil sensor has a rigid structure and square size of 1 m for deploying in a narrow underground space and is cooled at a low temperature of 77 K for improving the SNR. A theoretical calculation and an experimental test in an electromagnetically shielded room (EMSR) show that the optimal design of coil sensor consists of an 80-turn coil and a low-current-noise preamplifier AD745. It has a field sensitivity of 0.17 fT / Hz in the EMSR at 77 K, which is superior to the low temperature Superconducting Quantum Interference Device (LT SQUID) that is the latest application in MRS and the cooled coil with a diameter of 9 cm when detecting the laboratory NMR signal in kHz range. In the field experiment above the Taipingchi Reservoir near Changchun in China, the cooled coil sensor (CCS) developed in this paper has successfully obtained a valid weak MRS signal in high noise environment. The field results showed that the quality of measured MRS signal at 77 K is significantly superior to that at 298 K and the SNR is improved up to three times. This property of CCS makes the MRS instrument more convenient and reliable in a constricted space underground engineering environment (e.g., a mine or a tunnel).