Shen-yan-yi-hao oral solution ameliorates IgA nephropathy via intestinal IL-17/NF-κB pathway.

ETHNOPHARMACOLOGICAL RELEVANCE: Immunoglobulin A nephropathy (IgAN) is the most common primary glomerulonephritis in the world, it is one of the most common causes of kidney disease and can lead to end-stage kidney disease, however, its pathogenesis is still complicated. The Shen-yan-yi-hao oral solution (SOLI) is an effective prescription for the clinical treatment of IgAN while its specific mechanism remains to be further elucidated. AIM OF THE STUDY: This study investigates SOLI's effects on IgAN in rats, particularly on the intestinal mucosal barrier, and identifies potential therapeutic targets through network pharmacology and molecular docking, validated experimentally. MATERIALS AND METHODS: Target genes for SOLI in IgAN were identified and analysed through molecular docking and KEGG pathway enrichment. An IgAN rat model examined SOLI's effect on renal biomarkers and cytokines involved in specific pathways, ileum mucosal lesions, and the intestinal immune system. The IL-17 pathway's role was studied in IEC-6 cells with SOLI in vitro. RESULT: Rats developed increased proteinuria and kidney damage marked by IgA deposition and inflammation. SOLI treatment significantly ameliorated these symptoms, reduced galactose-deficient Ig A1 (Gd-IgA1), and decreased cytokines like IL-17, TNF-α, IL-6 and IL-1ß etc. SOLI also normalized intestinal tight junction protein expression, ameliorated intestinal damage, and regulated intestinal immune response (focused on IL-17/NF-κB signal pathway). SOLI moderated the abnormally activated IL-17 pathway, which damages intestinal epithelial cells, suggesting IgAN treatment potential. CONCLUSION: SOLI reduces proteinuria and enhances intestinal mucosal function in IgAN rats, kidney protection in the IgAN rat model may initiate from modulating the intestinal IL-17/NF-κB pathway and subsequent Gd-IgA1 accumulation.

Baicalein improves renal interstitial fibrosis by inhibiting the ferroptosis in vivo and in vitro.

Evidence indicates that Baicalein can ameliorate renal interstitial fibrosis by inducing myofibroblast apoptosis and inhibit the RLS3-induced ferroptosis in melanocytes. However, the relationship between renal interstitial fibrosis and anti-ferroptosis affected by Baicalein remains unclear. In our study, the anti-fibrosis and anti-ferroptosis effects of Baicalein were assessed in a rat model induced by the UUO method in vivo, and the effects of Baicalein on Erastin-induced ferroptosis of renal MPC-5 cells were examined by Western blot of fibrosis-related and ferroptosis-related proteins in vitro. In the UUO-induced rat model, Baicalein decreased kidney weight loss, improved renal function assessed the biomarks of urinary albumin excretion, serum creatine, and BUN levels, and reduced renal tubular injury. Furthermore, Baicalein inhibited renal ferroptosis by reducing ROS and MDA levels and increasing SOD and GSH levels in the UUO rat model. In addition, Baicalein potently reduced the expression of fibrosis-related proteins such as TGF-ß1, a-SMA, and Smad-2 to prevent renal interstitial fibrosis, and increased the expression of ferroptosis-related proteins such as SLC7A11, GPX4, and FTH to inhibit ferroptosis both in vitro and in vivo. Taken together, Baicalein exerts anti-fibrosis activity by reducing the ferroptosis response on the UUO-induced rat model and renal MPC5 cells. Therefore, Baicalein, as a novel therapeutic method on kidney diseases with strong activity in suppressing ferroptosis, could be a potential alternative treatment for renal interstitial fibrosis.

Design parameters and treatment efficiency of a retrofit bioretention system on runoff nitrogen removal.

Mixed media design is key factor that affects the operation of bioretention systems. In this study, four types of modifiers, namely, water treatment residual (WTR), green zeolite, fly ash, and coconut bran, were mixed with traditional bioretention soil (65% sand + 30% soil + 5% sawdust, by mass). Consequently, four kinds of modified media were obtained. Ten pilot-scale bioretention basins were constructed by setting different configurations. The steady infiltration rates of the modified packing bioretention systems were 3.25~62.78 times that of plant soil, which was 2.88~55.75 m/day. Results showed that the average concentration removal (ACR) of both mixed and layered fly ash and WTR were better than those of the other media, and the effects could reach over 61.92%. In the bioretention basins with WTR as the modifier, the treatment efficiency of nitrogen under the submerged zone height of 150 mm was relatively optimal, and ACR could reach 65.46%. Outflow total nitrogen (TN) load was most influenced by inflow load, and the correlation coefficient was above 0.765. Relative to the change of inflow concentration (IC), the change of recurrence interval (RI) and discharge ratio (DR) was more sensitive to TN load reduction. The reduction rate of TN load decreased by approximately 15% when the recurrence interval increased from 0.5 to 3 years. It decreased by approximately 12% when the discharge ratio increased from 10 to 20. This study will provide additional insights into the treatment performance of retrofit bioretention systems, and thus, can guide media and configuration design, effect evaluation, and related processes.

Triptolide Attenuates Podocyte Injury by Regulating Expression of miRNA-344b-3p and miRNA-30b-3p in Rats with Adriamycin-Induced Nephropathy.

Objectives. We investigated the action of triptolide in rats with adriamycin-induced nephropathy and evaluated the possible mechanisms underlying its protective effect against podocyte injury. Methods. In total, 30 healthy male Sprague-Dawley rats were randomized into three groups (normal group, model group, and triptolide group). On days 7, 28, 42, and 56, 24 h urine samples were collected. All rats were sacrificed on day 56, and their blood and renal tissues were collected for determination of biochemical and molecular biological parameters. Expression of miRNAs in the renal cortex was analyzed by a biochip assay and RT-PCR was used to confirm observed differences in miRNA levels. Results. Triptolide decreased proteinuria, improved renal function without apparent adverse effects on the liver, and alleviated renal pathological lesions. Triptolide also elevated the nephrin protein level. Furthermore, levels of miR-344b-3p and miR-30b-3p were elevated in rats with adriamycin-induced nephropathy, while triptolide treatment reversed the increase in the expression of these two miRNAs. Conclusions. These results suggest that triptolide may attenuate podocyte injury in rats with adriamycin-induced nephropathy by regulating expression of miRNA-344b-3p and miRNA-30b-3p.

[Contaminative conditions evaluation of sixteen main rivers flowing into sea around Bohai Sea, in summer of 2005].

In order to study the pollution conditions of sixteen main rivers around Bohai Sea such as Yellow River and so on, water samples were collected synchronously from July 1 to July 5 in summer of 2005. The results show that, thirteen rivers are worse than the IV water, and eight rivers such as Ziyaxinhe River and so on are worse than the V water. By analysing the sharing ratio of pollution, the key pollutant is petroleum (eleven rivers), the second is nutrients, and the third is permanganate index. The comprehensive trophic state index was assessed, and it is shown that seven rivers such as Liaohe River, Yellow River and so on are eutrophic, and the other seven rivers such as Xiaoqinghe River and Jiyunhe River and so on are extreme-eutrophic. As a result, the eutrophic comditions of rivers around Bohai Sea are very serious. The organic pollutant investigation indicates that the average concentration of TOC in sixteen rivers is 16.41 mg/L, the average permanganate index is 6.04 mg/L, and the average weight percentage of the easily degradable organics in total is only 15.61%, which suggests that the organic pollution conditions of the sixteen rivers are far serious, but the fluxes of total organic matters, which are more, should be put more attention on.