RESUMO
It has been reported that lncRNANR024118 can suppress lipopolysaccharide (LPS)induced inflammatory responses, which promote sepsis. The present study aimed to investigate the involvement of NR024118 in sepsis. Research subjects included 82 patients with sepsis without myocardial dysfunction (MD), 35 patients with sepsis with MD and 82 healthy controls. The expression levels of NR024118 in plasma collected from these participants and LPSinduced AC16 cells were measured by reverse transcriptionquantitative PCR. The expression levels of IL16 in these plasma samples and LPSinduced AC16 cells were measured by ELISA. The correlation between the expression levels of NR024118 and IL6 across plasma samples were analyzed by Pearson's correlation coefficient. The action potential duration (APD) was measured at 50 and 90% repolarization. Cell apoptosis was determined by cell apoptosis assay. The expression levels of ptranscription factor p65 were detected by western blot analysis. NFκB activity were analyzed by luciferase reporter assay. It was found that NR024118 was downregulated and IL6 was upregulated in the plasma of patients with sepsis. Among patients with sepsis, the individuals with MD exhibited even lower expression levels of NR024118 and higher expression levels of IL6. Among patients with sepsis with MD, the expression levels of NR024118 and IL6 were inversely correlated. LPS could induce MD to construct the sepsis models based on the increased expression levels of TNFα, IL1ß, IL6 and shortened APD by LPSmediated induction. Overexpression of NR024118 significantly reduced the secretion of IL6 and apoptosis of cardiomyocytes under LPS treatment. Functional studies demonstrated that NR024118 had negative regulation on p65 phosphorylation and NFκB activation. NR024118 was suppressed in sepsis and inhibited LPSinduced apoptosis of cardiomyocytes.
Assuntos
Apoptose/efeitos dos fármacos , Regulação para Baixo , Lipopolissacarídeos/efeitos adversos , Miócitos Cardíacos/metabolismo , RNA Longo não Codificante/metabolismo , Sepse/metabolismo , Adulto , Idoso , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Interleucina-1beta , Interleucina-6 , Masculino , Pessoa de Meia-Idade , NF-kappa B , RNA Longo não Codificante/genética , Transdução de Sinais/efeitos dos fármacos , Regulação para Cima/efeitos dos fármacosRESUMO
OBJECTIVE: To compare different nasal cavity nursing methods on mechanically ventilated patients. METHODS: According to acute physiology and chronic health evaluation (APACHEII), 615 cases of mechanically ventilated patients were divided into group A, group B and group C by stratified random method. Traditional oral nursing plus aspirating secretions from oral cavity and nasal cavity q6h were done in group A. Based on methods in group A, normal saline was used for cleaning nasal cavity in group B. Besides the methods in group A, atomizing nasal cleansing a6h was also used in group C. Incidence rate of Ventilator-Associated Pneumonia (VAP) and APACHE II scores after administrating were compared. The correlation between APACHE II score and outcomes was analyzed by Spearman-rank correlation. RESULTS: In group A, incidence of VAP was 36.76%, group B was 30.24%, group C was 20.38%, and the difference was statistically significant. APACHE II scores in group C were significantly lower compared with group A and B. APACHE II score was negatively correlated with clinical outcomes. CONCLUSIONS: For mechanically ventilated patients, nasal nursing can't be ignored and the new atomizing nasal cleaning is an effective method for VAP prevention.