Isolation and Characterization of the Wastewater Micropollutant Phenacetin-Degrading Bacterium Rhodococcus sp. Strain PNT-23.

Phenacetin, an antipyretic and analgesic drug, poses a serious health risk to both humans and aquatic organisms, which is of concern since this micropollutant is frequently detected in various aquatic environments. However, rare pure bacterial cultures have been reported to degrade phenacetin. Therefore, in this study, the novel phenacetin-degrading strain PNT-23 was isolated from municipal wastewater and identified as a Rhodococcus sp. based on its morphology and 16S rRNA gene sequencing. The isolated strain could completely degrade 100 mg/L phenacetin at an inoculum concentration of OD600 1.5 within 80 h, utilizing the micropollutant as its sole carbon source for growth. Strain PNT-23 exhibited optimal growth in LB medium at 37 °C and a pH of 7.0 with 1% NaCl, while the optimal degradation conditions in minimal medium were 30 °C and a pH of 7.0 with 1% NaCl. Two key intermediates were identified during phenacetin biodegradation by the strain PNT-23: N-acetyl-4-aminophenol and 4-aminophenol. This study provides novel insights into the biodegradation of phenacetin using a pure bacterium culture, expands the known substrate spectra of Rhodococcus strains and presents a potential new candidate for the microbial removal of phenacetin in a diverse range of environments.

A clustering review of vegetation-indicating parameters in urban thermal environment studies towards various factors.

People in outdoor areas suffer from more heat stress than indoors during warm seasons due to the lack of shelters or cooling facilities. This problem is pressing with urban heat island and continuous global warming. Researchers have explored various strategies for ameliorating thermal stress, coining the term 'outdoor thermal environment (OTE)' for this area of study. It has been found that the OTE is affected by vegetation and other factors (i.e., geometry) of a location. There have been many studies on vegetation, with these conducted at various levels and using different methods. Several parameters have been used to characterise vegetation and have been found to statistically correlate with many thermal indices (i.e., physiologically equivalent temperature, PET; universal thermal climate index, UTCI etc.). This article reports on a review of journal papers that investigated the climatic regulations of vegetation. In this study vegetation-indicating parameters were clustered according to the methods, scope, and thermal indices. Studies involving large scales preferred general indicators (e.g., NDVI, vegetation cover etc.) whereas specific, detailed parameters (e.g., crown sizes) were more frequently used in studies of micro levels. Outdoor thermal environment studies involving vegetation were mostly conducted in regions with high heat stress levels. Also, remote sensing and meteorological station observation were more frequently used in large-scale studies, while small-scale studies preferred simulation and field measurements. Their findings were expressed by the statistical correlation between vegetation parameters and thermal indices. For instance, NDVI, LAI, and crown size were negatively correlating with temperatures. The findings of this study help inform directions for future vegetation studies regarding outdoor thermal environment designs. Researchers would be clearer on selection methods and thermal indices regarding their targets and supporting tools.