RESUMO
Saikosaponin b2 (SSb2) can be extracted from Bupleurum spp. roots (Radix Bupleuri), which belongs to the Umbelliferae family. The current study aimed to explore the effects of SSb2 on proliferation of breast cancer cells and to identify the mechanism by which SSb2 affects breast cancer cell migration. mRNA expression levels of STAT3 and vasodilatorstimulated phosphoprotein (VASP) were determined and increased expression was observed in 16 breast cancer tissues compared with the paracancerous tissues. MTT, wound healing, colony formation assays and western blot suggested that SSb2 inhibited MCF7 proliferation and migration. It was further identified by western blot analysis that SSb2 treatment reduced levels of phosphorylated STAT3, VASP, matrix metallopeptidase (MMP) 2 and MMP9 in MCF7 compared with the untreated cells. In addition, it was demonstrated that inhibition of STAT3 phosphorylation decreased VASP expression levels and induction of STAT3 phosphorylation increased VASP levels. Furthermore, it was observed that the treatment of Kunming mice with SSb2 at 30 mg/kg/day for 30 days induced no obvious changes in the liver or kidney tissues, as determined by haematoxylin and eosin staining. In conclusion, these results indicated that SSb2 may be a potential antitumor drug for the treatment of breast cancer, which acts by suppressing proliferation and migration by downregulating the STAT3 signalling pathway and inhibiting the expression of VASP, MMP2 and MMP9 expression.
Assuntos
Neoplasias da Mama/tratamento farmacológico , Moléculas de Adesão Celular/genética , Proteínas dos Microfilamentos/genética , Ácido Oleanólico/análogos & derivados , Fosfoproteínas/genética , Fator de Transcrição STAT3/genética , Saponinas/farmacologia , Adolescente , Adulto , Animais , Neoplasias da Mama/genética , Neoplasias da Mama/patologia , Movimento Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Feminino , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Células MCF-7 , Metaloproteinase 9 da Matriz/genética , Camundongos , Pessoa de Meia-Idade , Ácido Oleanólico/farmacologia , Adulto JovemRESUMO
This study sought to investigate the effects of celecoxib on the proliferation and morphological changes of triple-negative breast cancer (TNBC) MDA-MB-231 cells. In this study, after MDA-MB-231 cells were treated with a certain concentration of celecoxib, a cell counting kit-8 (CCK-8) proliferation assay was used to detect cell viability. Western blotting was utilized to analyze the expression level of caspase-3, which is an apoptosis-related protein. In addition, the morphological changes in the cells and nuclei were determined with fluorescence and electron microscope. Apoptotic nuclei and obvious cytoplasmic vacuolization were observed with a microscope. Collectively, celecoxib can inhibit the proliferation of MDA-MB-231 cells by increasing caspase-3 expression and causing ultrastructural changes.
Assuntos
Celecoxib/farmacologia , Inibidores de Ciclo-Oxigenase 2/farmacologia , Neoplasias de Mama Triplo Negativas/ultraestrutura , Apoptose/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Feminino , Humanos , Microscopia Eletrônica de Transmissão , Neoplasias de Mama Triplo Negativas/patologiaRESUMO
OBJECTIVE: To study the chemical constituents of Lonicera japonica bud. METHODS: The compounds were isolated and repeatedly purified by silica gel column chromatography, gel column chromatography and ODS chromatography. The structures were elucidated by physicochemical properties, MS and NMR. RESULTS: Eight compounds were isolated and their structures were identified as protocatechuic acid (1), 5-hydroxy-7,3',4'-trimethoxyflavone (2), hyperoside (3), 4-hydroxycinnamic acid (4), ethyl caffeate(5), apigenin (6), 5-hydroxy-6,7,8,4'-tetramethoxyflavone (7), and ethyl laurate (8). CONCLUSION: Compounds 5, 7 and 8 are obtained from this genus for the first time.
