[Screening of hot-spot deafness gene mutations among 277 cochlear implantation patients in Sichuan province].

Objective:To investigate the spectrum and incidence of the hot-spot deafness gene mutations of 277 patients with cochlear implantation in Sichuan province, and to provide information of the prevention and treatment for clinical application. Method: The data of the hotspot deafness gene mutations screening of 277 patients with cochlear implantation was analyzed retrospectively. A deafness related gene mutations detection kit was used to detect 9 mutation sites in four deafness-associated genes,including GJB2(35delG,176del16,235delC,299delAT), GJB3(538C＞T),SLC26A4(2168A＞G, IVS7-2A＞G), Mitochondrial 12SrRNA(1494C＞T, 1555A＞G). Result: ① A total of 122 patients with hot-spot Deafness Gene Mutations were detected in 277 cochlear implantation patients(44.04%),among which there were 39 patients were GJB2235delC homozygous mutation(14.08%), 23 patients were GJB2 235delC heterozygous mutation(8.30%), 1 patient was GJB2 299delAT homozygous mutation(0.36%), 2 patients were GJB2 176del16& 235delC compound heterozygous mutation(0.72%), 13 patients were GJB2 235delC& 299delAT compound heterozygous mutation(4.69%), 2 patients were SLC26A4 2168A＞G heterozygous mutation(0.72%), 16 patients were SLC26A4 IVS7-2A＞G homozygous mutation(5.78%), 22 patient were SLC26A4 IVS7-2A＞G heterozygous mutation(7.94%), 1 patients was SLC26A4 2168A＞G& IVS7-2A＞G compound heterozygous mutation(0.36%), 2 patients were mitochondrial 12SrRNA gene 1555A＞G homogenous mutation(0.72%), 1 patient carried both GJB2 235delC homozygous mutation and SLC26A4 IVS7-2A＞G heterozygous mutation(0.36%). ②A total of 49 patients with LVAS were found in 277 cochlear implantation patients: including 15 patients with IVS7-2A＞G homozygous mutation(30.61%), 22 patients with IVS7-2A＞G heterozygous mutation(44.90%), 1 patient with 2168A＞G heterozygous mutation(2.04%), 1 patient with complex heterozygosis mutations of 2168A＞G and IVS7-2A＞G(2.04%), 1 patient with GJB2 235delC homozygous mutation(2.04%) and 1 patient with GJB2 235delC&299delAT compound heterozygous mutation(2.04%), and no hotspot deafness gene mutations were found in 8 patients. ③There were 40 out of 277 cochlear implantation patients with definite family history.There was no statistic difference of the detection rate of hot-spot deafness gene mutations between in patients with family deafness history (57.50%) and in patients without family deafness history (41.77%). ④A total of 273 patients with profound binaural deafness were found among 277 cochlear implantation patients. Three patients with profound deafness in right ear and severe deafness in left ear were found among 277 cochlear implantation patients.Two patients of three were SLC26A4 IVS7-2A＞G heterozygosis mutations, and one patient of three was GJB2 235delC heterozygosis mutations; 1 patient with profound deafness in left ear and severe deafness in right ear was found among 277 cochlear implantation patients,and was GJB2 235delC heterozygosis mutations. Conclusion:① The detection rate of hotspot deafness gene mutations in 277 cochlear implantation patients is 44.04%(122/277). GJB2 Mutation is the most common, SLC26A4 mutation takes the second place, mitochondrial 12SrRNAgene mutation is not common and GJB3 mutation is not found in this study.② SLC26A4 mutation may not be the sole pathogenic factor of LVAS. ③ The results of this study suggest that the genetic background of cochlear implants patients has little effect on the data of the hotspot deafness gene mutations screening.

Quantum fluctuations and stability of vortex lattices in a nonresonantly pumped exciton-polariton condensate.

The dynamics of an exciton-polariton condensate (EPC) subject to harmonic confinement can cause spontaneously formed vortices to arrange into a triangular vortex lattice. The stability of such a spontaneously formed vortex lattice is still unknown. We investigate the quantum fluctuations of vortex lattices in a rapidly rotating EPC with a rotation frequency close to the harmonic trap. In such a large condensate, we find that a vortex lattice with a triangular structure is stable, whereas one with a square structure becomes unstable. This result indicates that a driven-dissipative vortex array with strong quantum fluctuations can occur in an EPC.

[Clinical application and accuracy of the genioplasty surgical templates system for osseous genioplasty].

Objective: To develop and validate a new genioplasty templates system for monoblock osseous genioplasty. Methods: Thirty-six patients with chin deformities were enrolled in this study. The chin template system included a cutting guide and a repositioning guide for a genioplasty. Chin templates were designed in a computer and fabricated using a three-dimensional printing technique. The accuracy of the genioplasty templates were assessed by comparing the actual postoperative outcomes with the virtual plan. Results: All genioplasty was successfully completed by the template system. The largest linear root-mean-square deviation(RMSD) between the planned and the postoperative chin segments was 1.16 mm and the largest angular RMSD was 3.06°. Conclusions: The results showed that the chin template system provides a reliable method for transfer of genioplasty planning. The operation precision of the genioplasty can be improved by using the surgical templates system.

[Comparison between computer aided simulation and dental model orthognathic surgery for the treatment of patients with mandibular excess and facial asymmetries].

Objective: To evaluate the results of the orthognathic surgery with computer aided simulation-three-dimensional(3D) printed surgical guide and dental model surgery in the treatmemt of patients with mandibular excess and facial asymmetries. Methods: The coordinate system was built in ProPlan CMF 2.0 software, and the horizontal plane consisted of PoL, PoR, OrL, middle sagittal plane through nasion point and basion point and the plane perpendicular to the horizontal plane, coronoid plane through nasion point and the plane perpendicular to the horizontal plane and middle sagittal plane. The orientation of maxilla and mandibular distal segment was calculated by each triangle(U1-U6L-U6R, L1-L6L-L6R, Me-M5L-M5R) before and after orthognathic surgery. A total of 60 mandibular excess patients with facial asymmetries were enrolled in this retrospective study. They were divided into two groups, group Ⅰ with computer aided simulation, group Ⅱ with dental model surgery. The difference of maxillary occlusal plane roll and yaw angle, mandibular occlusal plane roll and yaw angle, and mandibular body plane roll and yaw angle were calculated. Statistical analysis was performed with SPSS 17.0 software. Results: The yaw angle of mandibular occlusal plane of the dental model surgery and computer aided simulation was 0.36°± 0.48° and 0.84° ± 0.36° (P=0.043), respectively. The roll angle of mandibular occlusal plane of the dental model surgery and computer aided simulation was 0.26°±0.79° and 0.54°±0.40°(P=0.032), respectively. The yaw angle of mandibular body plane of the dental model surgery and computer aided simulation was 0.60°± 1.04° and 0.23°±0.52°(P=0.008), respectively. The roll angle of mandibular body plane of the dental model surgery and computer aided simulation was 0.82° ± 0.72° and 0.53° ± 0.37° (P=0.028), respectively. The changes in computer aided simulation group were more obvious than that in the dental model surgery group, but the difference was not significant in the yaw angle of maxillary occlusal plane and the roll angle of maxillary occlusal plane between the two groups(P >0.05). Conclusions: It was more effective to correct mandibular asymmetry by computer aided simulation than dental model surgery.

Adomian's decomposition method for eigenvalue problems.

We extend the Adomian's decomposition method to work for the general eigenvalue problems, in addition to the existing applications of the method to boundary and initial value problems with nonlinearity. We develop the Hamiltonian inverse iteration method which will provide the ground state eigenvalue and the explicit form eigenfunction within a few iterations. The method for finding the excited states is also proposed. We present a space partition method for the case that the usual way of series expansion failed to converge.

Adomian's decomposition method for electromagnetically induced transparency.

We developed the Adomian's decomposition method to work for the electromagnetically induced transparency (EIT) problem. The method is general and capable to solve the coupled nonlinear partial differential equations for a light pulse passing through a three-level -type coherent medium. This EIT system is described by the coupled Maxwell-Schrödinger equations and optical Bloch equations. In the weak probe field case, the results agree with perturbation solutions and experimental data. In the stronger probe field case while perturbation may fail, our results reproduce experimental data well. With the techniques of spatial and time partitions, we extend the decomposition method that will be versatile for the investigation of the light pulse propagating through a coherent atomic medium.