RESUMO
Metal-organic frameworks (MOFs), especially bimetallic MOFs, have attracted widespread attention for simulating the structure and function of natural enzymes. In this study, different morphologies of bimetallic Cu-Zn-MOF with different peroxidase (POD)-like activities were prepared by simply controlling the molar ratio of Cu2+ and Zn2+. Among them, the doughnut-shaped Cu9-Zn1-MOF exhibited the largest POD-like activity. Cu9-Zn1-MOF was combined with glucose oxidase to construct a sensitive and selective glucose colorimetric biosensor with a linear detection range of 10-300 µM and a detection limit of 7.1 µm. Furthermore, Cu9-Zn1-MOF can efficiently convert hydrogen peroxide (H2O2) into hydroxyl radicals that effectively kill both gram-negative and gram-positive bacteria at low H2O2 level. The results of this study may promote the synthesis of bimetallic MOFs and broaden their applications in the biomedical field.
Assuntos
Antibacterianos , Colorimetria , Cobre , Glucose Oxidase , Glucose , Peróxido de Hidrogênio , Estruturas Metalorgânicas , Zinco , Colorimetria/métodos , Cobre/química , Estruturas Metalorgânicas/química , Zinco/química , Antibacterianos/farmacologia , Antibacterianos/química , Glucose/análise , Glucose/química , Glucose Oxidase/química , Glucose Oxidase/metabolismo , Peróxido de Hidrogênio/química , Técnicas Biossensoriais/métodos , Limite de Detecção , Peroxidase/química , Peroxidase/metabolismo , Testes de Sensibilidade MicrobianaRESUMO
Tartary buckwheat tea (TBT) is the most popular and widely consumed buckwheat product in many countries. However, the perfect quality control standards for TBT were still lacking, and the content of heavy metals in TBT and their health risks to consumers were still unknown. In this research, the total phenolic content, total flavonoid content, and antioxidant capacity as well as six metal contents and their health risks in TBT were detected. The results showed that the total phenolic content, total flavonoid content, and antioxidant capacity varied significantly among different types of TBT. Meanwhile, six metal concentrations in TBT leaves and infusions decreased in the order of Zn, Cu, Cr, Ni, Pb, and Cd. Health risk assessment indicated that the heavy metal intake only from TBT would not cause a noncarcinogenic risk to consumers. However, a strong carcinogenic risk of Cr in TBT for consumers should be paid more attention.