MiR-9 accelerates epithelial-mesenchymal transition of ovarian cancer cells via inhibiting e-cadherin.

OBJECTIVE: To investigate the influence of micro-ribonucleic acid (miR)-9 on epithelial-mesenchymal transition (EMT) of ovarian cancer cells by targeted inhibition on E-cadherin (CDH1). PATIENTS AND METHODS: The human ovarian cancer cells were cultured and miR-9 was repressed by inhibitors and overexpressed by miRNA mimics. The expression of EMT-related proteins was measured via Western blotting (WB). The action target of miR-9 was determined through the dual-luciferase reporter gene assay. The changes in protein levels were detected using WB. RESULTS: The expression of miR-9 was markedly up-regulated in ovarian cancer tissues, that is, the expression level of serum miR-9 in ovarian cancer patients was higher than that in control group. After the inhibition of miR-9, the expression level of epithelial indicator CDH1 was increased, while that of interstitial indicator Vimentin was decreased. MiR-9 contained a complementary site in the 3'-untranslated region (UTR) of CDH1 messenger RNA (mRNA) and the mRNA and protein expressions of CDH1 in the cells were down-regulated obviously by miR-9 overexpression. CONCLUSIONS: MiR-9 promotes the EMT of ovarian cancer cells through the targeted inhibition on CDH1.

[The multiple regression analysis of related factors in chronic rhinosinusitis postoperative recurrence].

Objective: To study the relative factors of endoscopic surgical effect in chronic rhinosinusitis. Method:The study included 179 chronic rhinosinusitis patients in our hospital who had a nasal endoscopic surgery from January 2013 to June 2014, and evaluate the treatment effect one year after the surgery，the patients were divided into two groups :recurrent and non-recurrent. Collecting clinical data and using uni-variate and multivariate analysis to find out the risk factors of recurrence, then the regression equation was established. Result:There are 29 cases of recurrence and 150 cases of non-recurrent in the total 179 chronic rhinosinusitis patients with a nasal endoscopic surgery.Uni-variate analysis showed that smoking，nasal polyps, allergic rhinitis,bronchial asthma,deviation of nasal septum, gastro-esophageal reflux disease,scores of VAS,maxillary sinus integral ,anterior ethmoid sinus integral,posterior ethmoid sinus integral,sphenoid sinus integral,frontal sinus integral,ostiomeatal complex integral,total sinus integral were statistically significant between chronic rhinosinusitis recurrent group and non-recurrent group(P <0.05). Multivariate analysis showed that smoking,nasal polyps, allergic rhinitis, bronchial asthma, deviation of nasal septum,scores of VAS,anterior ethmoid sinus integral,sphenoid sinus integral, ostiomeatal complex integral, total sinus integral were statistically significant between chronic rhinosinusitis recurrent group and non-recurrent group(P <0.05). Among those factors, smoking,nasal polyps, allergic rhinitis ,scores of VAS ,anterior ethmoid sinus integral,sphenoid sinus integral are the strongest in difference. Conclusion:The effect of chronic rhinosinusitis endoscopic surgery is not only related to clinical typing ,but also closely related to smoking,nasal polyps , allergic rhinitis and their severity.

[The clinical application of tinnitus handicap index(THI-12) Chinese version].

Objective:The original prospective of this study is to explore a convenient tinnitus severity assessment tool, using Chinese version of THI-12.Method:We surveyed 229 outpatients on their first hospital visits with primary tinnitus as chief complaint. Both the Chinese version THI-12 and the standard THI-25 were administrated. Their tinnitus grading and administration time were compared. The correlation between the two scores was evaluated. A reliability and factor analysis of the Chinese version of THI-12 was also performed.Result:Two hundred and fifteen of the 229 questionnaires were valid with a 93.9% response rate. The average administration time was(18.9±21.0) min for the standard THI-25 and(8.96±4.70)min for the Chinese version THI-12, which was significantly different. Pearson's correlation coefficient between the two total scores was r=0.833(P <0.01), which indicates a strong positive correlation.The tinnitus grading was not statistically significant(Z=-0.307，P >0.05).Cronbach's coefficient of THI-12 was α=0.765, suggesting good reliability and internal consistency. Factor analysis found three entries with characteristic values greater than 1. These three common factors explained 51.77% of the overall variance, suggesting that the 12 entries can be grouped into three dimensions(emotionality, sociality, concentration). Entry"Because of your tinnitus is it difficult for you to concentrate? "had the highest common value(0.78), suggesting that this entry would contribute the most should all entries be divided into three dimensions.Conclusion:The Chinese version THI-12 is time-efficient, has good reliability and internal structural validity, and provides good assessment of tinnitus severity.It can be widely applied in clinical practice.

[Determination of relative elements of hard metal in workplace air and urine by inductive coupled plama].

Objective: To establish a rapid detection method regarding the air conditions of workplace and the workers' urine included Tungsten, Cobalt, Nickel, Titanium, Cadmium, Manganese, Lead and its compounds based on inductively coupled plasma mass spectrometry (ICP-MS) . Methods: The experiment adopts ICP-MS to deter-mine those metals in workshop air and workers urine, evaluate the detection's limitation, the precision and accuracy of the method. Using the membrane filter and urine freeze - dried metal standard material to verify this method. Results: Each element of correlation coefficient was greater than 0.999. The recovery rate of air samples was 91.6%~104.6%, within-batch RSD precision was 1.41%~3.50%, between-run precision was 1.28%~4.31%, urine samples recovery rate was 93.0%~102.6%, within - batch RSD precision was 1.25%~3.56%, between - run precision was 1.58%~4.67%, According to the method every element was within the scope of the standard reference, it was also showed that the established method is accurate and reliable. Conclusion: ICP-MS is an effective and feasible method to detect the workshop air and the workers' urine which included Tungsten, Cobalt, Nickel, Titanium, Cadmium, Manganese, Lead and its compounds.