RESUMO
The relationship between Helicobacter pylori infection and gallbladder diseases, particularly cholecystitis and gallbladder polyps, remains unclear. This study aimed to investigate the presence of H. pylori in gallbladder tissues and its potential role in gallbladder pathologies, as well as to examine the expression of chemokines CXCL2 and CXCL5 in these conditions. MATERIAL AND METHODS: A total of 137 laparoscopically excised gallbladders were analysed through histological examination, PCR for H. pylori-specific DNA, and quantitative real-time PCR for CXCL2 and CXCL5 gene expression. The study cohort included patients with acute calculous cholecystitis, chronic calculous cholecystitis, and gallbladder polyps. RESULTS: H. pylori was detected in 30.7% of cases by histological methods and 42.3% by PCR. Elevated expression of CXCL2 and CXCL5 was observed in 62% and 57.7% of cases, respectively, with a higher prevalence in acute cholecystitis compared to chronic conditions. However, no statistically significant association was found between H. pylori presence and the forms of cholecystitis, as well as between H. pylori presence and chemokine expression in gallbladder. CONCLUSIONS: The study did not establish a direct link between the presence of H. pylori infection and forms of gallbladder pathologies. The findings suggest that other factors other than H. pylori may contribute to the upregulation of CXCL2 and CXCL5 in gallbladder diseases. Further research is needed to elucidate the complex interactions between H. pylori, chemokines, and gallbladder pathologies.
Assuntos
Quimiocina CXCL2 , Quimiocina CXCL5 , Vesícula Biliar , Infecções por Helicobacter , Helicobacter pylori , Humanos , Helicobacter pylori/isolamento & purificação , Helicobacter pylori/patogenicidade , Infecções por Helicobacter/microbiologia , Infecções por Helicobacter/patologia , Infecções por Helicobacter/complicações , Infecções por Helicobacter/genética , Masculino , Vesícula Biliar/microbiologia , Vesícula Biliar/patologia , Vesícula Biliar/cirurgia , Feminino , Pessoa de Meia-Idade , Quimiocina CXCL5/genética , Quimiocina CXCL5/metabolismo , Quimiocina CXCL2/genética , Quimiocina CXCL2/metabolismo , Adulto , Colecistite/microbiologia , Colecistite/patologia , Colecistite/cirurgia , Pólipos/microbiologia , Pólipos/patologia , Doenças da Vesícula Biliar/microbiologia , Doenças da Vesícula Biliar/patologia , Doenças da Vesícula Biliar/cirurgia , IdosoRESUMO
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