RESUMO
Chitosan/poly(DL-lactide-co-glycolide) (Ch/DL PLG) composite scaffolds were fabricated by freeze-drying lyophilization, and were evaluated and compared for use as a bone regeneration scaffold through measurements of the compression mechanical properties of the porous scaffolds. Also, In vitro cell culture of Sprague-Dawley rat's osteoblasts were used to evaluate the phenotype expression of cells in the scaffolds, characterizing the cellular adhesion, proliferation and alkaline phosphatase activity. The gene expression of osteocalcin, sialoprotein, alkaline phosphatase, Type I collagen and TGFß1 were confirmed in the samples; moreover, it was confirmed, the mineralization by IR spectra and EDS analysis. Our results thus show that Ch/DL PLG scaffolds are suitable for biological applications.
Assuntos
Quitosana/química , Teste de Materiais/métodos , Poliglactina 910/química , Engenharia Tecidual/métodos , Fosfatase Alcalina/biossíntese , Fosfatase Alcalina/metabolismo , Animais , Adesão Celular , Proliferação de Células , Colágeno Tipo I/metabolismo , Liofilização , Masculino , Osteoblastos/metabolismo , Osteocalcina/biossíntese , Fenótipo , Ratos , Ratos Sprague-Dawley , Sialoglicoproteínas/biossíntese , Fator de Crescimento Transformador beta1/metabolismoRESUMO
A high density lipoprotein (HDL) and beta-glucan binding protein (BGBP) have been found in the hemolymph of marine shrimp. These proteins are involved in the transport of lipid and the recognition of foreign matter, respectively. Similarities in the color of the proteins and the molecular mass were noted. For a detailed comparison, HDL and BGBP were purified from two shrimp species, Penaeus vannamei and Penaeus californiensis, and their biochemical characteristics determined. Both proteins from each of the shrimp species are monomeric with approximately the same molecular mass in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (approximately 100-112 kDa) and contain carbohydrate and lipid. The amino acid composition is similar and there is a high degree of similarity in the N-terminus. Furthermore, they are recognized by antibodies prepared independently. These results reveal that BGBP and HDL in shrimp hemolymph are the same protein, suggesting that there is a close relationship between the ability to respond to foreign matter and the diet as a provider of essential nutrients.