Mapping intellectual structure and research hotspots in the field of fibroblast-associated DFUs: a bibliometric analysis.

Background: Diabetic foot ulcers (DFUs) are one of the most popular and severe complications of diabetes. The persistent non-healing of DFUs may eventually contribute to severe complications such as amputation, which presents patients with significant physical and psychological challenges. Fibroblasts are critical cells in wound healing and perform essential roles in all phases of wound healing. In diabetic foot patients, the disruption of fibroblast function exacerbates the non-healing of the wound. This study aimed to summarize the hotspots and evaluate the global research trends on fibroblast-related DFUs through bibliometric analysis. Methods: Scientific publications on the study of fibroblast-related DFUs from January 1, 2000 to April 27, 2022 were retrieved from the Web of Science Core Collection (WoSCC). Biblioshiny software was primarily performed for the visual analysis of the literature, CiteSpace software and VOSviewer software were used to validate the results. Results: A total of 479 articles on fibroblast-related DFUs were retrieved. The most published countries, institutions, journals, and authors in this field were the USA, The Chinese University of Hong Kong, Wound Repair and Regeneration, and Seung-Kyu Han. In addition, keyword co-occurrence networks, historical direct citation networks, thematic map, and the trend topics map summarize the research hotspots and trends in this field. Conclusion: Current studies indicated that research on fibroblast-related DFUs is attracting increasing concern and have clinical implications. The cellular and molecular mechanisms of the DFU pathophysiological process, the molecular mechanisms and therapeutic targets associated with DFUs angiogenesis, and the measures to promote DFUs wound healing are three worthy research hotspots in this field.

Diastereoselective Synthesis of Bicyclo[3.3.0]octenones by Copper-Catalyzed Transannular Ring-Closing Reaction.

A novel and efficient copper-catalyzed transannular ring-closing reaction of eight-membered rings has been developed that provides a straightforward way to synthesize bicyclo[3.3.0]octane derivatives in good yields. Mechanistic studies revealed that the reaction pathway might involve chlorination followed by the Kornblum reaction. Readily accessible starting materials and good functional group tolerance make this procedure attractive.

Ecological efficiency evaluation and driving factor analysis of the coupling coordination of the logistics industry and manufacturing industry.

The coupling coordination of the logistics industry and manufacturing industry is conducive to the sustainable development of logistics and manufacturing and the stability of sustainable supply chain. The logistics and manufacturing industries are not only the basic industries that support social development, but also the industries with high carbon emissions. This paper constructs the measurement system of coupling coordinating ecological efficiency of two industries based on carbon emission constraints and finds out the driving factors affecting ecological efficiency, which is of great significance to the low-carbon coordinated development of the two industries in the future. Firstly, this paper classifies the carbon emissions from the logistics industry and manufacturing industry as undesirable outputs and evaluates the ecological efficiency of the logistics industry (LEE) and manufacturing industry (MEE) in the three urban agglomerations from 2006 to 2019 by using the unexpected slacks-based measure (SBM) model. Secondly, the coupling coordination method is used to analyze the coupling coordination scheduling of industrial ecological efficiency (MLCC). Finally, the spatial econometric model is used to analyze the driving factors of the MLCC. The results show that during the study period, the coupling coordination of the three urban agglomerations continued to grow, the Pearl River Delta coupling coordination is the highest, the Yangtze River Delta coupling coordination grew the fastest, and the Beijing-Tianjin-Hebei coupling coordination grew slightly slower. The development during the 13th Five-Year Plan period is obviously faster than that during the 11th and 12th Five-Year Plan. The empirical analysis results of spatial econometrics show that the driving factors have an impact on the coupling coordination degree of the three urban agglomerations, but the significance of each factor is different. The driving factors have significant spatial heterogeneity. The three urban agglomerations should formulate low-carbon industry development policies in line with local development according to the actual situation of each region and local conditions.

Epigallocatechin-3 gallate regulates macrophage subtypes and immunometabolism to ameliorate experimental autoimmune encephalomyelitis.

Epigallocatechin-3 gallate (EGCG) is a polyphenolic component of tea and has potential curative effects in patients with autoimmune diseases. Multiple sclerosis (MS) is an autoimmune disease affecting the central nervous system (CNS). It remains unknown whether EGCG can regulate macrophage subtypes in MS. Here we evaluated the effects of EGCG in experimental autoimmune encephalomyelitis (EAE), MS mouse model. We found that EGCG treatment reduced EAE severity and macrophage inflammation in the CNS. Moreover, EAE severity was well correlated with the ratio of M1 to M2 macrophages, and EGCG treatment suppressed M1 macrophage-mediated inflammation in spleen. In vitro experiments showed that EGCG inhibited M1 macrophage polarization, but promoted M2 macrophage polarization. These effects were likely to be related to the inhibition of nuclear factor-κB signaling and glycolysis in macrophages by EGCG in macrophages. Overall, these findings provided important insights into the mechanisms through which EGCG may mediate MS.

miRNA-467b inhibits Th17 differentiation by targeting eIF4E in experimental autoimmune encephalomyelitis.

Multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE) are neuroinflammatory autoimmune diseases characterized by the axonal loss, demyelination, and neurodegeneration of the central nervous system. Overactivation of CD4+ T cells, especially the migration of the Th1 and Th17 subsets into the central nervous system (CNS), leads to the secretion of inflammatory mediators and destruction of the contact between neurons and activated macrophages, which can then result in a series of neurocognitive and motor deficits. In this study, we intended to explore the role of miRNA-467b in regulating Th cell development in EAE. We found that the level of miRNA-467b was decreased and eukaryotic initiation factor 4 F (eIF4E) was increased in lymph nodes and the CNS at EAE peak. eIF4E was confirmed as the direct target of miRNA467b. Overexpression of miRNA-467b could suppress a percentage of CD4+ IL-17+ cells in EAE CD4 + T cells in vitro. In addition, we also identified miRNA-467b, which could suppress Th17 cell differentiation by targeting eIF4E in vitro. Furthermore, injecting miRNA-467b mimics into the caudal vein of EAE mice contributed to less inflammation in the peripheral lymphoid organs and CNS and alleviated disease severity. Taken together, our findings imply that miRNA-467b inhibits the differentiation and function of Th17 cells by targeting eIF4E, thereby alleviating EAE.

The Effect of Lipid Metabolism on CD4+ T Cells.

CD4+ T cells play a vital role in the adaptive immune system and are involved in the pathogenesis of many diseases, including cancer, autoimmune diseases, and chronic inflammation. As an important mechanism for energy storage, a lot of researches have clarified that metabolism imbalance interacts with immune disorder, and one leads to the other. Lipid metabolism has close relationship with CD4+ T cells. In this review, we discuss fatty acid, cholesterol, prostaglandin, and phospholipid metabolism in CD4+ T cell subsets. Fatty acid ß-oxidation (FAO) is activated in Th17 cell to support the proinflammatory function. Cholesterol promotes Th1, Th2, and Treg cell differentiation. In addition to glucose metabolism, lipid metabolism is also very important for immunity. Here, it is highlighted that lipid metabolism regulates CD4+ T cell differentiation and function and is related to diseases.

[Hydrothermal synthesis and luminescence properties of CePO4 : Tb flower-like clusters].

Tb(3+)-doped CePO4 flower-like clusters were hydrothermally synthesized without using any template or surfactant by varying the reactant Tb3+ cation concentration. It was observed that the flower-like clusters were composed by nanowires with a diameter of about 80-90 nm and lengths up to 1 microm. The structures, morphologies, sizes and luminescence properties of the products were studied by X-ray powder diffraction (XRD), field-emission scanning electronic microscopy (FE-SEM), and luminescence spectra. With the reactant Ce3+ /Tb3+ molar ratio of 0.850 : 0.150, the uniform flower-like clusters were actually composed of a self-assembly of the oriented nanowires through an Tb(3+)-induced in the excessive PO4(3-). It was found that the reactant Ce3+/Tb3+ molar ratio and phosphoric acid played key roles in the morphology control of the product. A possible formation mechanism for the flower-like morphology was also proposed. The luminescence properties of CePO4 : Tb flower-like cluster were performed, indicating that the strongest emission intensity was reached with 0.850 : 0.150 molar ratios of Ce3+/Tb3+.