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BACKGROUND:Based on the previous studies,the New Zealand rabbit talar chondrocyts were isolated,cultured and identified in vitro.OBJECTIVE:To explore the isolation,culture and identification of New Zealand rabbit talar chondrocyts in vitro.METHODS:The chondrocyts were isolated from the talar cartilage of New Zealand white rabbits by type Ⅱ collagen enzyme digestion,and then cultured in vitro.The cells were identified by inverted phase contrast microscope,toluidine blue staining and collagen type Ⅱ immunohistochemical staining.RESULTS AND CONCLUSION:Under the inverted phase contrast microscope,most of passaged chondrocytes presented with polygonal or triangle shape and had round or oval nuclei.Toluidine blue staining showed the hyacinthine chondrocytes and blue cellular matrix.Collagen type Ⅱ immunohistochemical staining showed that the chondrocytes appeared with brown granules in the cytoplasm and membrane.To conclude,a system that can isolate,culture and identify talar chondrocytes from New Zealand rabbits is successfully established.Talar chondrocytes at passages 1-3 grow well and have stable biological properties.
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Objective: To observe the changes of Cajal interstitial cells(CIC) in the intestine of rats with liver cirrhosis, so as to study the mechanism of gastrointestinal dysfunction. Methods: Twenty Wistar rats were equally randomized into control group and cirrhosis model group. Cirrhosis model was established by CCl4 in rats. The intestinal motility changes were assayed using Dextran blue-2000 as an indicator. Immunohistochemical staining was used to investigate the distribution of c-kit positive CIC in jejunum of rats. Meanwhile, the ultrastructure of CIC was observed by electron microscope. Results: Compared with control group, the intestinal motility and the c-kit positive CIC in jejunum were both markedly decreased in model group(both P< 0.01). The CIC, with less organelles and dissovled cytoplasm, had decreased connections with other cells and damaged structure. Conclusion: The intestinal motility of rats with liver cirrhosis is significantly decreased, which may be associated with the changes of CIC number and ultrastructure in intestine.
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Objective To study the mechanisms of Pericarpium Arecae. Methods A total of Wistar rats were randomly divided into Pericarpium Arecae group and control group, Pericarpium Arecae decoction or distilled water were given respectively. Changes of gastrointestinal motility rats were assayed by Dextran blue-2000 after 1 and 6 hours. The distributions of SP and VIP in antrum and jejunum were investigated by immunohistochemistry assay. Results The gastrointestinal motility of rats was markedly enhanced (P<0.01 or P<0.05). The expressions of SP increased significantly (P<0.01 or P<0.05) and the expressions of VIP decreased significantly (P<0.01 or P<0.05) in antrum and jejunum of rats at 1 and 6 h after Pericarpium Arecae decoction was given. The changes were more obvious at 1 h than at 6 h. Conclusion The kinetogenic effect of Pericarpium Arecae is closely correlated to the increase of SP expression and the decrease of VIP expression in gastrointestinal tract.
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Objective To study the mechanisms of Pericarpium Arecae. Methods A total of Wistar rats were randomly divided into Pericarpium Arecae group and control group, Pericarpium Arecae decoction or distilled water were given respectively. Changes of gastrointestinal motility rats were assayed by Dextran blue-2000 after 1 and 6 hours. The distributions of SP and VIP in antrum and jejunum were investigated by immunohistochemistry assay. Results The gastrointestinal motility of rats was markedly enhanced (P<0.01 or P<0.05). The expressions of SP increased significantly (P<0.01 or P<0.05) and the expressions of VIP decreased significantly (P<0.01 or P<0.05) in antrum and jejunum of rats at 1 and 6 h after Pericarpium Arecae decoction was given. The changes were more obvious at 1 h than at 6 h. Conclusion The kinetogenic effect of Pericarpium Arecae is closely correlated to the increase of SP expression and the decrease of VIP expression in gastrointestinal tract.