Suppression of photoreactivation of E. coli by excimer far-UV light (222 nm) via damage to multiple targets.

Low-pressure mercury lamps emitting at 254 nm (UV254) are used widely for disinfection. However, subsequent exposure to visible light results in photoreactivation of treated bacteria. This study employed a krypton chloride excimer lamp emitting at 222 nm (UV222) to inactivate E. coli. UV222 and UV254 treatment had similar E. coli-inactivation kinetics. Upon subsequent irradiation with visible light, E. coli inactivated by UV254 was reactivated from 2.71-log to 4.75-log, whereas E. coli inactivated by UV222 showed negligible photoreactivation. UV222 treatment irreversibly broke DNA strands in the bacterium, whereas UV254 treatment primarily formed nucleobase dimers. Additionally, UV222 treatment caused cell membrane damage, resulting in wizened, pitted cells and permeability changes. The damage to the cell membrane was mainly due to the photolysis of proteins and lipids by UV222. Furthermore, the photolysis of proteins by UV222 destroyed enzymes, which blocked photoreactivation and dark repair. The multiple damages can be further evidenced by 4.0-61.1 times higher quantum yield in the photolysis of nucleobases and amino acids for UV222 than UV254. This study demonstrates that UV222 treatment damages multiple sites in bacteria, leading to their inactivation. Employing UV222 treatment as an alternative to UV254 could be viable for inhibiting microorganism photoreactivation in water and wastewater.

Assessing the Chemical-Free Oxidation of Trace Organic Chemicals by VUV/UV as an Alternative to Conventional UV/H2O2.

Low-pressure mercury lamps with high-purity quartz can emit both vacuum-UV (VUV, 185 nm) and UV (254 nm) and are commercially available and promising for eliminating recalcitrant organic pollutants. The feasibility of VUV/UV as a chemical-free oxidation process was verified and quantitatively assessed by the concept of H2O2 equivalence (EQH2O2), at which UV/H2O2 showed the same performance as VUV/UV for the degradation of trace organic contaminants (TOrCs). Although VUV showed superior H2O activation and oxidation performance, its performance highly varied as a function of light path length (Lp) in water, while that of UV/H2O2 proportionally decreased with decreasing H2O2 dose regardless of Lp. On increasing Lp from 1.0 to 3.0 cm, the EQH2O2 of VUV/UV decreased from 0.81 to 0.22 mM H2O2. Chloride and nitrate hardly influenced UV/H2O2, but they dramatically inhibited VUV/UV. The competitive absorbance of VUV by chloride and nitrate was verified as the main reason. The inhibitory effect was partially compensated by •OH formation from the propagation reactions of chloride or nitrate VUV photolysis, which was verified by kinetic modeling in Kintecus. In water with an Lp of 2.0 cm, the EQH2O2 of VUV/UV decreased from 0.43 to 0.17 mM (60.8% decrease) on increasing the chloride concentration from 0 to 15 mM and to 0.20 mM (53.5% decrease) at 4 mM nitrate. The results of this study provide a comprehensive understanding of VUV/UV oxidation in comparison to UV/H2O2, which underscores the suitability and efficiency of chemical-free oxidation with VUV/UV.

Ozone, hydrogen peroxide, and peroxymonosulfate disinfection of MS2 coliphage in water.

The control of viruses in water is critical to preventing the spread of infectious viral diseases. Many oxidants can inactivate viruses, and this study aims to systematically compare the disinfection effects of ozone (O3), peroxymonosulfate (PMS), and hydrogen peroxide (H2O2) on MS2 coliphage. The effects of oxidant dose and contact time on disinfection were explored, as were the disinfection effects of three oxidizing agents in secondary effluent. The 4-log inactivation of MS2 coliphage required 0.05 mM O3, 0.5 mM PMS, or 25 mM H2O2 with a contact time of 30 min. All three oxidants achieved at least 4-log disinfection within 30 min, and O3 required only 0.5 min. In secondary effluent, all three oxidants also achieved 4-log inactivation of MS2 coliphage. Excitation-emission matrix (EEM) results indicate that all three oxidants removed dissolved organic matter synchronously and O3 oxidized dissolved organic matter more thoroughly while maintaining disinfection efficacy. Considering the criteria of oxidant dose, contact time, and disinfection efficacy in secondary effluent, O3 is the best choice for MS2 coliphage disinfection among the three oxidants.

Dose-Response Behavior of Pathogens and Surrogate Microorganisms across the Ultraviolet-C Spectrum: Inactivation Efficiencies, Action Spectra, and Mechanisms.

The dose-response behavior of pathogens and inactivation mechanisms by UV-LEDs and excimer lamps remains unclear. This study used low-pressure (LP) UV lamps, UV-LEDs with different peak wavelengths, and a 222 nm krypton chlorine (KrCl) excimer lamp to inactivate six microorganisms and to investigate their UV sensitivities and electrical energy efficiencies. The 265 nm UV-LED had the highest inactivation rates (0.47-0.61 cm2/mJ) for all tested bacteria. The bacterial sensitivity strongly fitted the absorption curve of nucleic acids at wavelengths of 200-300 nm; however, indirect damage induced by reactive oxygen species (ROS) was the leading cause of bacterial inactivation under 222 nm UV irradiation. In addition, the guanine and cytosine (GC) content and cell wall constituents of bacteria affect inactivation efficiency. The inactivation rate constant of Phi6 (0.13 ± 0.002 cm2/mJ) at 222 nm due to lipid envelope damage was significantly higher than other UVC (0.006-0.035 cm2/mJ). To achieve 2log reduction, the LP UV lamp had the best electrical energy efficiency (required less energy, average 0.02 kWh/m3) followed by 222 nm KrCl excimer lamp (0.14 kWh/m3) and 285 nm UV-LED (0.49 kWh/m3).

Migration of rare and abundant species, assembly mechanisms, and ecological networks of microbiomes in drinking water treatment plants: Effects of different treatment processes.

Microorganisms play an important role in the degradation of pollutants. However, they also cause problems in drinking water distribution systems, such as pipe corrosion and biofilm growth. The microbial assembly mechanisms and molecular ecological networks associated with different drinking water treatment processes have not yet been clearly analyzed. Therefore, this study investigated the microbiomes of three processes (coal filtration-activated carbon, ozone-activated carbon and UV, and ozone-activated carbon) during different seasons. The results showed that the microbial composition and diversity among the different processes and during different seasons. Water treatment processes had deterministic effects on the microbial assembly process and significantly changed the composition of rare and abundant species, altering the size and modules of molecular ecology networks. Rare species considered as keystone species play important roles in microbial ecology and microbial community construction. Ozone-activated carbon and UV/chlorination decreased the bacterial concentration, increased the deterministic process of microbial assembly, and significantly reduced the size of the network, which is of great significance to microbial control in drinking water. This research broadens our perspectives on the microbial assembly associated with drinking water treatment processes and contributes to ensuring the safe supply of drinking water.

Antibiotic resistome alteration along a full-scale drinking water supply system deciphered by metagenome assembly: Regulated by seasonality, mobile gene elements and antibiotic resistant gene hosts.

Both drinking water treatment processes and distribution can lead to antibiotic resistome variation, yet the variation of antibiotic resistome in the whole drinking water supply system (DWSS) combined with seasonality remains unknown. In this study, microbial community, antibiotic resistome, mobile genetic elements (MGEs) co-existing with antibiotic resistance genes (ARGs) and ARG hosts would be explored along a DWSS for four seasons with metagenome assembly. Multidrug and bacitracin ARGs were dominant ARGs in DWSS. Integrase, plasmids, recombinase and transposase were major MGEs co-existing with ARGs. Filtration and disinfection treatments could alter the ARG relative abundance, mainly via changing the abundance of ARG hosts (Limnohabitans and Polynucleobacter), which was influenced by water total organic carbon (TOC) content. When TOC was relatively high, filtration could proliferate ARGs via promoting antibiotic resistance bacteria (ARB) but chlorine dioxide could decrease ARGs via killing ARB. Filtration played an important role in controlling ARGs by reducing ARB when TOC was relatively low. The stimulation effect of disinfection on ARGs existed in more oligotrophic environment. Distribution could enrich ARGs in higher temperature by increasing MGEs co-occurring with ARGs and diversifying ARG hosts. MGEs co-occurring with ARGs became more abundant and diverse in disinfected water in warmer seasons. Microbial community was the most important factor determining the antibiotic resistome along a DWSS. These findings extend the knowledge about how and why water treatment processes and pipe distribution shape drinking water antibiotic resistome in different seasons.

Seasonal variations of microbial community and antibiotic resistome in a suburb drinking water distribution system in a northern Chinese city.

Antibiotic resistance genes (ARGs) are an emerging issue for drinking water safety. However, the seasonal variation of ARGs in drinking water distribution systems (DWDS) is still unclear. This work revealed the tempo-spatial changes of microbial community, ARGs, mobile genetic elements (MGEs) co-occurring with ARGs, ARG hosts in DWDS bulk water by means of metagenome assembly. The microbial community and antibiotic resistome varied with sampling season and site. Temperature, ammonia, chlorite and total plate count (TPC) drove the variations of microbial community structure. Moreover, environmental parameters (total organic carbon (TOC), chlorite, TPC and hardness) shifted antibiotic resistome. ARGs and MGEs co-occurring with ARGs showed higher relative abundance in summer and autumn, which might be attributed to detached pipe biofilm. In particular, ARG-bacitracin and plasmid were the predominant ARG and MGE, respectively. ARG hosts changed with season and site and were more diverse in summer and autumn. In winter and spring, Limnohabitans and Mycobacterium were the major ARG hosts as well as the dominant genera in microbial community. In addition, in summer and autumn, high relative abundance of Achromobacter and Stenotrophomonas were the hosts harboring many kinds of ARGs and MGEs at site in a residential zone (0.4 km from the water treatment plant). Compared with MGEs, microbial community had a greater contribution to the variation of antibiotic resistome. This work gives new insights into the dynamics of ARGs in full-scale DWDS and the underlying factors.

Molecular ecological networks reveal the spatial-temporal variation of microbial communities in drinking water distribution systems.

Microbial activity and regrowth in drinking water distribution systems is a major concern for water service companies. However, previous studies have focused on the microbial composition and diversity of the drinking water distribution systems (DWDSs), with little discussion on microbial molecular ecological networks (MENs) in different water supply networks. MEN analysis explores the potential microbial interaction and the impact of environmental stress, to explain the characteristics of microbial community structures. In this study, the random matrix theory-based network analysis was employed to investigate the impact of seasonal variation including water source switching on the networks of three DWDSs that used different disinfection methods. The results showed that microbial interaction varied slightly with the seasons but was significantly influenced by different DWDSs. Proteobacteria, identified as key species, play an important role in the network. Combined UV-chlorine disinfection can effectively reduce the size and complexity of the network compared to chlorine disinfection alone, ignoring seasonal variations, which may affect microbial activity or control microbial regrowth in DWDSs. This study provides new insights for analyzing the dynamics of microbial interactions in DWDSs.

Can we shape microbial communities to enhance biological activated carbon filter performance?

A new focus on biofiltration has emerged that aims to shape microbial communities to improve treatment efficacy. It is therefore necessary to understand the linkages between microbial community structure and biofilter function. However, the assembly and interaction of microbial communities in biological activated carbon (BAC) filters are unknown. In this study, we selected one coal-based granular activated carbon (GAC), GAC-13, with simultaneously developed micropore and micro-level macropore volume used for a bench-scale BAC column experiment, and compared it with other coal-based GACs and wood-based GAC in terms of the dissolved organic carbon (DOC) removal and microbial community characteristics. The results showed that there was no difference between the DOC removal efficiency of BAC-13 and the other two coal-based BAC filters with high iodine value in the period dominated by adsorption, while the DOC removal efficiency of BAC-13 (64.7±0.6%) was significantly higher than that of other BAC filters (36.3±0.8-54.1±0.4%) with a difference of 0.3-0.7 mg/L in DOC during the steady state. The bacterial communities were strongly assembled by deterministic rather than stochastic factors, where the surface polarity of GAC had a greater effect on the microbial communities than its physical properties. The corresponding co-occurrence network revealed that microbes in the BAC filter may be more cooperative than competitive. The keystone bacterium Hyphomicrobium, which had a relatively low abundance, contributed 0.3-1% more to the most abundant functions and produced 5-21 proteins/(g·GAC) more than the dominant bacterium Sphingobium. The metaproteomic-based approach could provide more accurate information regarding the contributions of different species to metabolic functions. The pore size distribution of GAC was found to be an important factor in determining BAC filter performance; the most important pore sizes were micropores and micro-level macropores (0.2-10 µm and >100 µm in diameter), and the latter impacted the abundance of keystone species. Overall, our findings provide new insights into shaping microbial communities by optimizing pore size structure to improve BAC performance, especially the abundance of keystone species.

Implication on selection and replacement of granular activated carbon used in biologically activated carbon filters through meta-omics analysis.

Biologically activated carbon (BAC) filters are widely used in China and worldwide as an essential part of advanced water treatment. However, it is unclear how to properly select the granular activated carbon (GAC) used in BAC filters and to determine when GAC should be replaced. In this study, five BAC filters, each filled with a different coconut- or coal-based GAC with different physicochemical properties, were run continuously for 400 days. The structure and function of the microbial community and the quantity of specific enzymes in the BAC filters were investigated through an integrated metagenomic/metaproteomic analysis. The results indicated that GAC adsorption still played a major role in removing organic matter once the filters reached a steady-state, which was attributed to bioregeneration, and the contribution of adsorption might be relatively greater than that of biodegradation. GAC with strong adsorption capacity and high bioregeneration potential selected bacterial communities more phylogenetically closely-related than others. The iodine value could be used as an indicator of BAC performance in terms of organic matter removal in the initial stage of the filters, which is dominated by adsorption. However, it could not be used to assess performance at a later stage when adsorption and biodegradation occurred simultaneously. Pore-size distribution characteristics could be chosen as a potential better indicator compared with the current adsorption indicators, dually representing the adsorption performance and the microbial activity, and the proportion of important pore-size of GAC that is more suitable for BAC filter is suggested. GAC with strongly polar terminal groups is more conducive to the removal of ammonium-nitrogen.

Microbial composition and diversity of drinking water: A full scale spatial-temporal investigation of a city in northern China.

The microbiological water quality of drinking water distribution systems (DWDSs) is of primary importance for public health. The detachment of biofilm attached on the pipe wall attribution to water source switch and the occurrence of potentially pathogenic chlorine-resistant bacteria (CRB) under chlorine disinfection get lots of attention. Studies examining microbial communities after the water source switch, particularly in low-salinity water, have been scant. The UV­chlorine combined disinfection applied in one of the investigated drinking water plants provided insight into the control of CRBs. We applied high-throughput sequencing of the 16S rRNA gene to characterize the bacterial communities of the DWDS in northern China over 1 year. A network comprising four different DWDSs was sampled at 48 sites every season (temperate continental monsoon climate), and the impact of key spatial-temporal and physicochemical parameters was investigated. Overall, the entire bacterial community was not significantly different among the four DWDSs (spatial parameter) but varied with seasons (temporal parameter). The switch in water sources might increase the relative abundance of potentially opportunistic pathogens in DWDSs. UV­chlorine combined disinfection can decrease community diversity and is likely to control the growth of potential opportunistic pathogens in DWDSs.

Successional Dynamics of Molecular Ecological Network of Anammox Microbial Communities under Elevated Salinity.

Response of microbial interactions to environmental perturbations has been a central issue in wastewater treatment system. However, the interactions among anammox microbial community under salt perturbation is still unclear. Here, we used random matrix theory (RMT)-based network analysis to investigate the dynamics of networks under elevated salinity in an anammox system. Results showed that high salinity (20 and 30 g/L NaCl) inhibited anammox performance. Salinity led to closer and more complex networks for the overall network and subnetwork of Planctomycetes and Proteobacteria, especially under low salinity (5 g/L NaCl), which could serve as a strategy to survive under salt perturbation. Planctomycetes, most dominant phylum and playing crucial roles in anammox, possessed higher proportion of competitive relationships (64.3%) under 30 g/L NaCl. OTU 109 (closely related to Ignavibacterium), the only network hub detected in the anammox system, also had larger amount of competitive relationships (27.3%) than the control (0%) under 30 g/L NaCl. Similar result was found for the most abundant keystone bacteria Candidatus Kuenenia. These increasing competitions at different taxa level could be responsible for the deterioration of nitrogen removal. Besides, all the network topological features tended to reach the values of the original network, which showed the network of microbial community could gradually adapt to the elevated salinity. Microbial network analysis adds a different dimension for our understanding of the response in microbial community to elevated salinity.

Microbial network succession along a current gradient in a bio-electrochemical system.

A lab-scale three dimensional biofilm-electrode reactor (3DBER) coupled with sulfur/iron (3DBER-Fe/S) system was established to examine the impacts of current gradient on the performances and microbial network dynamics. Results showed that generally low current could promote nitrogen and phosphorus removal, while high current caused the inhibition of nutrients removal. Molecular ecological network (MEN) analysis showed that the current altered the overall architecture of the networks, and low currents could improve the scale and complexity of networks (<100 mA), while high current (≥100 mA) likely decrease the networks scale and complexity. Stronger competition was observed among Proteobacteria and Chloroflexi at high current conditions, which may be relevant to the deterioration of nutrients removal. In addition, the current dramatically altered the network interactions among denitrifiers, and the keystone species were intensively dynamic among various networks under the current gradient.

Effect of granular activated carbon pore-size distribution on biological activated carbon filter performance.

Proper granular activated carbon (GAC) selection could improve the performance of biological activated carbon (BAC) filters through a combination of adsorption and biodegradation, while the GACs used in BAC filters are now mainly selected according to adsorption function, ignoring biodegradation. In this study, sand filter effluent obtained from a drinking water treatment plant was fed into continuous-flow bench-scale BAC columns operated in parallel over 245 days to examine the effects of GAC pore-size distribution on BAC filter performance, in terms of the dissolved organic carbon (DOC) and disinfection byproduct (DBP) precursors. A metagenomic analysis indicated that bacterial community structure played an important role in BAC filter performance. A significant correlation was found between metabolism-related proteins and the volume of micro-level macropores based on metaproteomic analysis. It is suggested that the adsorption saturation was dynamic and that adsorption played a role in the performance of the BAC filters throughout the 245-day operating period. Renewed adsorption capacity, or bioregeneration, was driven by bacterial metabolic activity. Such activity largely depended on the organic matter adsorbed by the GAC, in which micro-level macropores, especially those with diameters of 0.2-10 µm, played an important but previously unrecognized role. The results suggest that more attention should be paid to well-developed pores and pore-size distribution in the production and selection of GAC used for full-scale drinking water biofilters.