Transcriptome analyses reveal the potential mechanisms for color changes of a sweet orange peel induced by Candidatus Liberibacter asiaticus.

'Shatangju' mandarin (Citrus reticulate Blanco cv. Shatangju) is a Chinese citrus specialty in southern China with a delicious taste and an attractive appearance. Huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus (CLas) threatens the Shatangju industry seriously. Fruits from citrus trees with HLB show 'red nose' peels with a serious reduction in fruit value. Differentially expressed genes (DEGs) have been identified in the leaves of several citrus species with HLB infection. However, similar studies on the fruit peels of citrus trees with HLB infection are very limited. In this study, the pathogen CLas was diagnosed in the 'red nose' fruit peels of Shatangju. The chlorophyll and carotenoid contents in different peels were also analyzed. Besides, we identified DEGs in the comparison between peels from normal red-colored and 'red nose' fruits via RNA-seq. A total of 1922 unigenes were identified as DEGs, of which 434 were up-regulated and 1488 were down-regulated in the 'red nose' fruit peels. DEGs involved in chlorophyll and carotenoids biosynthesis, photosynthesis, and transcription factors could be responsible for fruit color changes after HLB infection. Our findings provide a preliminary understanding of the mechanism underlying the formation of a 'red nose' on fruit peel from HLB-infected trees.

The bacterial and fungal communities of the larval midgut of Spodoptera frugiperda (Lepidoptera: Noctuidae) varied by feeding on two cruciferous vegetables.

Spodoptera frugiperda is a highly polyphagous pest worldwide with a wide host range that causes serious losses to many economically important crops. Recently, insect-microbe associations have become a hot spot in current entomology research, and the midgut microbiome of S. frugiperda has been investigated, while the effects of cruciferous vegetables remain unknown. In this study, the growth of S. frugiperda larvae fed on an artificial diet, Brassica campestris and Brassica oleracea for 7 days was analyzed. Besides, the microbial community and functional prediction analyses of the larval midguts of S. frugiperda fed with different diets were performed by high-throughput sequencing. Our results showed that B. oleracea inhibited the growth of S. frugiperda larvae. The larval midgut microbial community composition and structure were significantly affected by different diets. Linear discriminant analysis effect size (LEfSe) suggested 20 bacterial genera and 2 fungal genera contributed to different gut microbial community structures. The functional classification of the midgut microbiome analyzed by PICRUSt and FUNGuild showed that the most COG function categories of midgut bacterial function were changed by B. oleracea, while the guilds of fungal function were altered by B. campestris significantly. These results showed that the diversity and structure of the S. frugiperda midgut microbial community were affected by cruciferous vegetable feeding. Our study provided a preliminary understanding of the role of midgut microbes in S. frugiperda larvae in response to cruciferous vegetables.