RESUMO
Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.
Assuntos
Bacteriemia/microbiologia , Fasciite Necrosante/microbiologia , Infecções por Bactérias Gram-Negativas/diagnóstico , Diálise Peritoneal Ambulatorial Contínua/efeitos adversos , Peritonite/microbiologia , Shewanella putrefaciens/isolamento & purificação , Idoso , Antibacterianos/uso terapêutico , Ceftazidima/uso terapêutico , Evolução Fatal , Gentamicinas/uso terapêutico , Humanos , Masculino , Insuficiência de Múltiplos Órgãos/microbiologiaRESUMO
The present work is a preliminary experiment for hydrocyclones as a primary process in lake sediment thickening. A few cyclones based on the Rietema standard geometry were prepared, and sample particles were sediments from a local lake and waste coal fly ash for a reference test. As a result of the chemical analysis, more organic contaminants were found in smaller particles. The experimental tests showed that physical characteristics of particles, configuration of the cyclone and operation conditions could affect the separation efficiency.