RESUMO
A rapid medium for the detection of lysine and ornithine decarboxylase and arginine dihydrolase activity of 439 strains of gram-negative, nonfermenting bacteria was evaluated and compared with Moeller decarboxylase medium. Results were obtained in 4 to 24 h using the rapid medium, whereas Moeller medium often required extended (3 to 7 days) incubation. There was 100% agreement in the lysine tests with both media and almost 100% agreement in the ornithine tests. There was 91% agreement in the arginine tests, with the significance of discrepant results discussed. The sensitivity, specificity, and quick results obtained by the rapid test make it a suitable substitute for Moeller medium for the identication of gram-negative, nonfermenting bacteria.
Assuntos
Carboxiliases/metabolismo , Meios de Cultura , Hidrolases/metabolismo , Ornitina Descarboxilase/metabolismo , Arginina/metabolismo , Estudos de Avaliação como Assunto , Fermentação , Lisina/metabolismo , Ornitina/metabolismo , Especificidade da EspécieRESUMO
Studies on the incorporation of three (14)C-labeled amino acids into preparations of partially purified Anaplasma marginale were conducted. Relatively high levels of uptake of radioactive isoleucine, glycine, and methionine were detected in both trichloroacetic acid-soluble and -insoluble fractions of these preparations, indicating that the parasite is capable of protein synthesis outside its erythrocytic environment. When erythrocytes (red blood cells) from infected calves were incubated with [(14)C]methionine and Anaplasma bodies were subsequently purified, it was found that some of the label detected was associated with the marginal bodies.
