Grain shattering by cell death and fracture in Eragrostis tef.

Abscission, known as shattering in crop species, is a highly regulated process by which plants shed parts. Although shattering has been studied extensively in cereals and a number of regulatory genes have been identified, much diversity in the process remains to be discovered. Teff (Eragrostis tef) is a crop native to Ethiopia that is potentially highly valuable worldwide for its nutritious grain and drought tolerance. Previous work has suggested that grain shattering in Eragrostis might have little in common with other cereals. In this study, we characterize the anatomy, cellular structure, and gene regulatory control of the abscission zone (AZ) in E. tef. We show that the AZ of E. tef is a narrow stalk below the caryopsis, which is common in Eragrostis species. X-ray microscopy, scanning electron microscopy, transmission electron microscopy, and immunolocalization of cell wall components showed that the AZ cells are thin walled and break open along with programmed cell death (PCD) at seed maturity, rather than separating between cells as in other studied species. Knockout of YABBY2/SHATTERING1, documented to control abscission in several cereals, had no effect on abscission or AZ structure in E. tef. RNA sequencing analysis showed that genes related to PCD and cell wall modification are enriched in the AZ at the early seed maturity stage. These data show that E. tef drops its seeds using a unique mechanism. Our results provide the groundwork for understanding grain shattering in Eragrostis and further improvement of shattering in E. tef.

Psychiatry Resident Training in Cultural Competence: An Educator's Toolkit.

Resident physicians training in psychiatry in the U.S. are required to master a body of knowledge related to cultural psychiatry; are expected to adopt attitudes that endorse the principles of cultural competence; and finally are expected to acquire specific cultural competence skills that facilitate working effectively with diverse patients. This article first provides an overview of the Accreditation Council for Graduate Medical Education (ACGME) competencies related to cultural competence, as well as the American Academy of Child and Adolescent Psychiatry's (AACAP) recommendations for the cultural competence training of child/adolescent fellows. Next, numerous print and electronic resources that can be used in cultural competence education in psychiatry are reviewed and discussed. Finally, we conclude by providing recommendations for psychiatry residency programs that we culled from model cultural competence curricula.

Training Psychiatry Residents in Psychotherapy: The Role of Manualized Treatments.

Evidence-based treatment and manualized psychotherapy have a recent but rich history. As interest and research have progressed, defining the role of treatment manuals in resident training and clinical practice has become more important. Although there is not a universal definition of treatment manual, most clinicians and researchers agree that treatment manuals are an essential piece of evidence-based therapy, and that despite several limitations, they offer advantages in training residents in psychotherapy. Requirements for resident training in psychotherapy have changed over the years, and treatment manuals offer a simple and straightforward way to meet training requirements. In a search limited to only depression, two treatment manuals emerged with the support of research regarding both clinical practice and resident training. In looking toward the future, it will be important for clinicians to remain updated on further advances in evidence based manualized treatment as a tool for training residents in psychotherapy, including recent developments in online and smartphone based treatments.

Accrediting Graduate Medical Education in Psychiatry: Past, Present, and Future.

The current terminology, goals, and general competency framework systematically utilized in the education of residents regardless of specialty is almost unrecognizable and quite foreign to those who trained before 2010. For example, the clinical and professional expectations for physicians-in-training have been placed onto a developmental framework of milestones. The expectations required during training have been expanded to include leadership and team participation skills, proficiency in the use of information technology, systems-based knowledge including respect of resources and cost of care, patient safety, quality improvement, population health and sensitivity to diversity for both individual and populations of patients. With these additions to physician training, the Accreditation Council for Graduate Medical Education (ACGME) hopes to remain accountable to the social contract between medicine and the public. With a focus on psychiatric practice, this article provides a general background and overview of the major overhaul of the accreditation process and educational goals for graduate medical education and briefly highlights possibilities for the future.

Training Residents in the Use of Telepsychiatry: Review of the Literature and a Proposed Elective.

Many regions in the United States are deficient in mental health services, especially those in rural areas. As a result of these deficiencies, many patients in need of psychiatric services are often left untreated. Although it is clear that telepsychiatry has great potential in improving patient access to mental health care in areas where psychiatric services are deficient, the lack of familiarity with the technology and inadequate training are current barriers to expanding the use of telepsychiatry. A review of telepsychiatry, its clinical applications, and evidence-based literature regarding competencies in graduate medical education related to telepsychiatry are provided. An approach to implementing telepsychiatry into a curriculum is suggested. We also propose an elective clinical experience with resources for didactics or independent study that will enable residents to develop a knowledge base and competence in the practice of telepsychiatry.

Biochemical characteristics of organic matter in a guano concretion of late miocene or pliocene age from manchester parish in Jamaica.

The biogeochemical fate of organic matter (OM) entering soils is an important issue that must be examined to better understand its roles in nitrogen cycling and as a natural modulator of soil-atmospheric carbon fluxes. Despite these critical roles, there are uncertainties in estimating the contribution of this feedback mechanism due in part to a lack of molecular-level information regarding the origin and labile and refractory inventories of OM in soils. In this study, we used a multi-analytical approach to determine molecular-level information for the occurrence and stabilization of OM in a bird guano concretion of the Late Miocene or Pliocene age in Jamaica. We determined the specific organic structures persisting in the concretion and the possible contribution of fossil organic matter to the OM pool in modern environments. Our results indicate that aliphatic species, presumably of a highly polymethylenic nature [(CH2)n], may significantly contribute to the stable soil-C pool. Although not as significant, proteins and carbohydrates were also enriched in the sample, further suggesting that fossil organic matter may contribute to carbon and nitrogen pools in present day soil organic matter.

Mapping the critical service needs of adolescent children of prisoners.

This study explores the service needs of adolescent children of prisoners by collecting information directly from the youth. The conceptual and empirical literature focused on children of prisoners generally attempts to describe or understand the effects of parental incarceration on prisoners' offspring but frequently fails to include data gathered directly from youth or include their voices in the litany of suggested programs and services. This study employed concept mapping technology to investigate the service needs of 14 adolescent children of prisoners living in an urban city in the Midwest. The findings revealed perceptions of unmet physical, emotional, psychological, and social needs divided into six thematic clusters. The findings also suggest that a single-service focus such as mentoring or tutoring may fail to meet the multiple and varied needs that these youth identified, especially those related to basic care such as food and clothing. Participants were asked to rate the themes on importance, and data suggest a high level of consistency between young women and young men. Strength-based solutions were then identified from the data to meet the needs of these youth.

Sibling family practices: guidelines for healthy boundaries.

A questionnaire was given to 500 mental health and child welfare professionals asking for maximum acceptable ages for siblings to engage jointly in certain family practices related to hygiene, affection, and privacy. A large proportion of respondents felt it was never acceptable for siblings to take showers together (40%), kiss on the mouth (37%), or toilet together (32%). Some significant differences occurred based on the gender of the older sibling within sets of same gender or mixed gender pairs, with older brothers being acceptable up to lower ages than older sisters. The effects of child abuse, age, race, and the amount of education on the respondents' answers are investigated. The limitations of the age guidelines are discussed.

Different paths to death row: a comparison of men who committed heinous and less heinous crimes.

Part of the answer to violent crime prevention is to understand the route that those who have committed violent crimes have traveled in order to find ways to guide others from the road leading to such violence. An investigation of the lifelong personal and environmental factors affecting 37 men who were executed in 1997 focuses on distinctions between men in two categories based on heinousness of violent crime. The study aimed to identify risk factors and events that preceded the violent event and to compare the constellation of variables of the men who committed particularly heinous murders characterized by extreme rage and brutality with those whose crimes and criminal histories were characterized mostly by property crimes without intentional harm to people. Descriptive results suggest differences between the two groups of men related to 19 variables and the emergence of two diverse profiles of risk factors and life experiences.

Boundaries and family practices: implications for assessing child abuse.

Family practices related to hygiene, affection behavior, and privacy were studied using a sample of mental health and child welfare professionals. The professionals were asked to use their own experience to state up to what age it was acceptable for parents and children of the same gender and mixed gender to engage in certain family practices. For virtually all family practices, respondents reported lower appropriate ages for mixed gender pairs. Family practices were acceptable for mothers with their daughters up to older ages than fathers with their sons. Results indicate high variability in the responses regarding appropriate ages, as well as whether the behavior was ever acceptable. The implications of these substantial differences among professionals who often assess these practices as "soft signs" related to abuse are discussed.