Estimation of tile drainage contribution to streamflow and nutrient loads at the watershed scale based on continuously monitored data.

Nitrogen losses from artificially drained watersheds degrade water quality at local and regional scales. In this study, we used an end-member mixing analysis (EMMA) together with high temporal resolution water quality and streamflow data collected in the 122 km2 Otter Creek watershed located in northeast Iowa. We estimated the contribution of three end-members (groundwater, tile drainage, and quick flow) to streamflow and nitrogen loads and tested several combinations of possible nitrate concentrations for the end-members. Results indicated that subsurface tile drainage is responsible for at least 50% of the watershed nitrogen load between April 15 and November 1, 2015. Tiles delivered up to 80% of the stream N load while providing only 15-43% of the streamflow, whereas quick flows only marginally contributed to N loading. Data collected offer guidance about areas of the watershed that should be targeted for nitrogen export mitigation strategies.

The first confirmed cases of full albinism in rajid species.

Three albino skate specimens (Rajidae) were captured from the North Sea and English Channel between 2008 and 2011. Using DNA barcoding (COI gene) and morphometric analyses, species were identified as a spotted ray Raja montagui, a blonde ray Raja brachyura and a thornback ray Raja clavata. This finding represents the first record of full albinism (a lack of skin and retinal pigmentation) in rajid species.

Low genetic diversity in a snail intermediate host (Biomphalaria pfeifferi Krass, 1848) and schistosomiasis transmission in the Senegal River Basin.

Population genetic perturbations of intermediate hosts, often a consequence of human pressure on environmental resources, can precipitate unexpectedly severe disease outbreaks. Such disturbances are set to become increasingly common following range changes concomitant with climate shifts, dwindling natural resources and major infrastructure changes such as hydroprojects. Construction of the Diama dam in the Senegal River Basin (SRB) reduced river salinity, enabling the freshwater snail intermediate host Biomphalaria pfeifferi to rapidly expand its distribution. A serious public health problem ensued, with an epidemic of intestinal schistosomiasis occurring in the previously schistosome-free Richard-Toll region within 2 years. The current study aimed to assess the population variability of B. pfeifferi in the SRB, and speculate upon its subsequent impact on host-parasite interactions following such engineered ecological change. Genetic variation at nine polymorphic microsatellite loci revealed little population differentiation in SRB snails compared with those from natural habitats in Zimbabwe, where Schistosoma mansoni transmission is much lower. 'Open' SRB habitats are associated with greater water contact, smaller population sizes and less genetic diversity, with sites downstream of Richard-Toll showing greater inter- and intrapopulation variation, concomitant with less frequent human contact. These observations may be explained by rapid expansion into pristine habitat selecting for high fecundity genotypes at the expense of schistosome resistance, presenting S. mansoni with genetically homogenous highly fecund susceptible populations around the focal point, promoting development of a highly compatible host-parasite relationship. Longitudinal study of such systems may prove important in predicting public health risks engendered by future environmental engineering projects.

Size-dependent growth of individual Atlantic salmon Salmo salar alevins from hatch to first feeding.

Variation in egg size, hatch timing and size at hatch, and their influence on individual growth rates of Atlantic salmon Salmo salar alevins up to first feeding were examined in pure strain and hybrid crosses of fish from Scotland and Canada. At the intra-female, intra-cross type and inter-cross type levels, specific growth rates prior to first feed were strongly size dependent, with smaller and later hatching alevins growing significantly faster. The magnitude of this size-dependent growth was greatest in the hybrid crosses. This resulted in a 40% reduction in the coefficient of variation (c.v.) in alevin size from post-hatch to first feeding at the intra-female level, and a reduction of both intra- and inter-cross differences in alevin sizes in the same period.

Leaf shape linked to photosynthetic rates and temperature optima in South African Pelargonium species.

The thermal response of gas exchange varies among plant species and with growth conditions. Plants from hot dry climates generally reach maximal photosynthetic rates at higher temperatures than species from temperate climates. Likewise, species in these environments are predicted to have small leaves with more-dissected shapes. We compared eight species of Pelargonium (Geraniaceae) selected as phylogenetically independent contrasts on leaf shape to determine whether: (1) the species showed plasticity in thermal response of gas exchange when grown under different water and temperature regimes, (2) there were differences among more- and less-dissected leafed species in trait means or plasticity, and (3) whether climatic variables were correlated with the responses. We found that a higher growth temperature led to higher optimal photosynthetic temperatures, at a cost to photosynthetic capacity. Optimal temperatures for photosynthesis were greater than the highest growth temperature regime. Stomatal conductance responded to growth water regime but not growth temperature, whereas transpiration increased and water use efficiency (WUE) decreased at the higher growth temperature. Strikingly, species with more-dissected leaves had higher rates of carbon gain and water loss for a given growth condition than those with less-dissected leaves. Species from lower latitudes and lower rainfall tended to have higher photosynthetic maxima and conductance, but leaf dissection did not correlate with climatic variables. Our results suggest that the combination of dissected leaves, higher photosynthetic rates, and relatively low WUE may have evolved as a strategy to optimize water delivery and carbon gain during short-lived periods of high soil moisture. Higher thermal optima, in conjunction with leaf dissection, may reflect selection pressure to protect photosynthetic machinery against excessive leaf temperatures when stomata close in response to water stress.

The effects of lubrication on the temperature rise and surface finish of amalgam and composite resin.

UNLABELLED: It was thought that when finishing and polishing direct filling materials lubrication would affect the surface roughness and temperature rise in samples of amalgam and composite. OBJECT: Previous work by the authors has shown that there is an optimum load, speed and time that produced the smoothest surface when finishing amalgam and composite resin using each of four grades of a disc system. This work was undertaken to examine the effects on temperature rise in samples of amalgam and composite resin of finishing dry compared to finishing with different lubricants. The experiments all used these optimum loads, speeds and times. It also compares the surface finish produced using different lubricants. MATERIALS AND METHODS: A high copper amalgam and a hybrid composite resin were finished using the four grades of abrasive discs. Samples produced were 25 mm long by 6 mm wide by 2 mm deep. A thermocouple was inserted 1 mm into the base of the samples. The thermocouple was connected via an electronic thermometer to a computer that permitted the display and recording of temperature against time. After roughening, the samples were finished and polished in a specially constructed jig that mimicked oral finishing. The pre-determined optimum loads, speeds and times were used sequentially for each of the four grades of disc. Five samples were tested for each method of finishing. Firstly, run dry, then in turn lubricated with water, walnut oil and petroleum jelly. After the use of each abrasive disc the surface roughness was measured. One of the five samples was selected at random and prepared for examination in the scanning electron microscope. All results were subjected to non-parametric statistically analyses. RESULTS: With both materials the temperature rise was greatest when run dry, followed by petroleum jelly, walnut oil and the least was when lubricated with water. With these two materials the surface roughness correlates negatively with the temperature rise. The smoothest surface being achieved when finished dry. CONCLUSIONS: To obtain the smoothest surface finish amalgam and composite should be finished dry but further work is needed to assess the effect of the temperature rise found in the materials on the pulp.

The effects of lubrication on the temperature rise and surface finish of glass-ionomer cements.

OBJECT: Previous work [Jones CS. Factors influencing the finishing of direct filling materials. PhD Thesis, University of London; 2002] has shown that there is an optimum load, speed and time that produced the smoothest surface when finishing glass-ionomer cement using each of four grades of a disc system. This study looks at the effects of lubrication on the temperature produced in samples of GIC when finished dry and with different lubricants using these optimal loads, speeds and times. It also compares the surface finish produced using different lubricants. MATERIALS AND METHODS: A thermocouple connected so that it permitted the display and recording of temperature against time was inserted 1mm into the base of samples of a glass-ionomer cement. The samples were finished and polished using each of the grades of a disc system in a specially constructed jig that mimicked oral finishing. After roughening, the pre-determined optimum loads, speeds and times were used sequentially for each of the four grades of disc. Five samples were tested for each method of finishing. Firstly run dry, then in turn lubricated with water, walnut oil and petroleum jelly. After the use of each abrasive disc the surface roughness was measured using a profilometer. One of the five samples was selected at random and prepared for examination in the scanning electron microscope. All results were subjected to non-parametric statistically analyses. RESULTS: Walnut oil and petroleum jelly produced significant temperature increases compared to both dry and with water finishing. Lubricated with water significantly reduced the temperature rise compared to dry. The Ra values of 0.5 microm was obtained for the coarse and a value of 0.3 microm for the medium discs run without lubrication. With lubrication the Ra increased although there was little difference between the lubricants. However the photomicrographs showed that walnut oil and petroleum jelly caused gross morphological changes indicating major surface destruction. CONCLUSIONS: The practice of finishing GICs using petroleum jelly or similar lubricant appears to be detrimental. Further experimental work needs to be done to advise practitioners on finishing GICs to produce the smoothest surface possible.

Effect of dietary alpha-lipoic acid on growth, body composition, muscle pH, and AMP-activated protein kinase phosphorylation in mice.

The effects of alpha-lipoic acid (ALA) on the growth, body composition, postmortem AMP-activated protein kinase (AMPK) activation, and 24-h muscle pH were investigated. Thirty male C57BL/6J mice were fed diets containing 0, 0.5, or 1.0% ALA (DM basis). At the end of the 3-wk feeding trial, carcass weights decreased (P < 0.05) 14 and 30% for mice fed 0.5 and 1.0% ALA, respectively, compared with the 0% group, with decreases in BW as the levels of dietary ALA increased. This change in carcass weight occurred because carcass fat content for mice receiving 0.5 and 1.0% ALA was 7.32 and 8.09% lower (P < 0.05), respectively, than for the 0% ALA treatment, and because gonadal fat decreased (P < 0.05) 85% in mice fed 1.0% ALA compared with those fed 0% ALA. Dietary ALA caused a slight increase (P < 0.05) in carcass moisture content, with no (P = 0.07) effect on protein and ash content. Furthermore, ALA supplement decreased (P < 0.05) ADFI (DM basis) from 4.3 g/d for 0% ALA-fed mice to 3.4 g/d for 1.0% ALA-fed mice. At 20 min postmortem, pH was greater (P < 0.05) in muscle of mice fed 1.0% ALA than in muscle of mice fed 0% ALA. Ultimate (24-h) pH values differed (P < 0.05) among treatments, and mean values were 5.83, 6.08, and 6.29 for 0, 0.5, and 1.0% ALA, respectively. Phosphorylation of AMPK alpha subunit at Thr172, an indicator of AMPK activation, was decreased (P < 0.05) in muscle of ALA-treated mice at 20 min postmortem. Because AMPK has a crucial role in the control of glycolysis, the reduction in AMPK activation decreases glycolysis, and thereby increases the ultimate pH of postmortem muscle. In summary, dietary ALA supplement can decrease fat accumulation in mice, and because ALA increased muscle pH at 20 min and 24 h postmortem, these results suggest that dietary ALA supplementation might decrease carcass fatness and prevent the development of PSE pork and poultry. However, further research is required to test the effects of ALA in swine and poultry.

Laboratory study of the loads, speeds and times to finish and polish direct restorative materials.

This study investigated the loads, speeds and times required to achieve the smoothest surface on samples of amalgam, composite resin and glass-ionomer cement using the four grades of a disc polishing system. The tests were conducted on a specially constructed jig. The restorations were abraded with the appropriate disc using a range of loads, speeds and times based on a previous practitioner study. The surface roughness of each material was measured before and after abrading. The load was varied first, followed by speed and finally time. Photomicrographs were taken of a sample finished at the optimum load, speed and time and of loads above and below the optimum that produced the smoothest surface finish. In addition the fractured edge of a sample finished at the optimum values was examined. There was an optimum load, speed and time that produced the smoothest surface for each restorative material. For amalgam and composite the surface roughness value decreased as the discs became finer. There was a reduction in the roughness value for glass-ionomer cement using the two roughest discs. The values obtained were substantially in contrast from the earlier practitioner study. There were specific values for load, speed and time that produced a smoother surface for each material.

Breeding system and demography shape population genetic structure across ecological and climatic zones in the African freshwater snail, Bulinus forskalii (Gastropoda, Pulmonata), intermediate host for schistosomes.

The role of breeding system and population bottlenecks in shaping the distribution of neutral genetic variation among populations inhabiting patchily distributed, ephemeral water bodies was examined for the hermaphroditic freshwater snail Bulinus forskalii, intermediate host for the medically important trematode Schistosoma guineensis. Levels of genetic variation at 11 microsatellite loci were assessed for 600 individuals sampled from 19 populations that span three ecological and climatic zones (ecozones) in Cameroon, West Africa. Significant heterozygote deficiencies and linkage disequilibria indicated very high selfing rates in these populations. Despite this and the large genetic differentiation detected between populations, high levels of genetic variation were harboured within these populations. The high level of gene flow inferred from assignment tests may be responsible for this pattern. Indeed, metapopulation dynamics, including high levels of gene flow as well as extinction/contraction and recolonization events, are invoked to account for the observed population structuring, which was not a consequence of isolation-by-distance. Because B. forskalii populations inhabiting the northern, Sahelian area are subject to more pronounced annual cycles of drought and flood than the southern equatorial ones, they were expected to be subject to population bottlenecks of increased frequency and severity and, therefore, show reduced genetic variability and elevated population differentiation. Contrary to predictions, the populations inhabiting the most northerly ecozone exhibited higher genetic diversity and lower genetic differentiation than those in the most southerly one, suggesting that elevated gene flow in this region is counteracting genetic drift.

The advantages and disadvantages of running a clinical trial in general practices.

This paper reviews the experiences encountered in running a clinical trial on the use of a metal reinforced glass-ionomer cement in general dental practices. The practitioners were asked to place both the test material and amalgam in the same patient and to take impressions both at placement and at three recall intervals. Subsequently plaster casts were produced from these impressions. These were then assessed by three independent observers to provide evaluation of the relative wear of the two restoratives under evaluation. A commercial laboratory manufactured the models on which the three independent observers carried out the assessment of wear. Although the practitioners indicated they would be able to provide the number of restorations required in a relatively short period these expected numbers were never achieved. Despite recruitment of additional participants the trial never did achieve the number of restorations required. The performance of the metal reinforced glass ionomer appeared to vary dependent on the practitioner placing the filling. Questioning of the participants found that some participants were finishing the material using a method specifically contraindicated in the protocol for the trial, the directions supplied with the product, and in briefing sessions held prior to the trial. This implies that there can be major problems in undertaking clinical trials of this nature in the general dental service and has serious clinical implications for those contemplating this type of evaluation and for manufacturers introducing new products.

The in vivo perception of roughness of restorations.

OBJECTIVE: To determine a threshold of detection value for surface roughness of restorations by patients using their tongue. METHOD: Samples of composite resin were finished with differing grades of abrasive. The surface roughness (Ra) was measured and representative scanning electro-micrographs taken. These were compared with labial enamel. Twenty-five volunteers were asked to rank them in order of perceived roughness using the tip of their tongue. RESULTS: These showed that the 60% of volunteers were able to rank the specimens correctly, and were able to distinguish differences in roughness values from between 0.25 and 0.50 microm. This range encompasses that of natural enamel. CONCLUSIONS: The subjects were able to distinguish lower roughness values than have previously been reported. It is concluded that when finishing restorations the surface should have a maximum roughness 0.50 microm if it is not to be detected by the patient.

The expression of immune-regulatory genes in rainbow trout, Oncorhynchus mykiss, during a natural outbreak of proliferative kidney disease (PKD).

Proliferative kidney disease (PKD) is a parasitic infection of salmonid fish characterized by an apparently abnormal immune response to the presence of the myxozoan parasite, Tetracapsuloides bryosalmonae. In order to examine the nature of the immune response at the molecular level, the expression of a range of immune regulatory genes, including cytokines and cyclooxygenase (COX)-2 was examined in naive unexposed fish and in naive fish exposed to parasite-infected water at three points during the course of a natural outbreak of PKD. Since fish with advanced PKD pathology generally exhibit increased susceptibility to secondary infections which is typical of stress/cortisol-mediated immune suppression, a further aim of this work was to examine in vitro the influence of the glucocorticoid cortisol on the bacterial lipopolysaccharide (LPS)-induced expression of the trout cytokine genes studied. Two weeks after the initial sampling, naive exposed fish showed a specific profile of up-regulated tumor necrosis factor (TNF)-alpha2, COX-2 and, to a lesser extent, transforming growth factor (TGF)-beta1 expression. As the disease pathology increased, TNF-alpha2 and COX-2 expression returned to normal levels. Stress levels of cortisol suppressed the LPS inducibility of pro-inflammatory cytokine genes, although TGF-beta1 and TNF-alpha2 appeared to be refractory. These data demonstrate that specific immune responses at the molecular level are affected during PKD infection, with the cortisol suppression of cytokine expression in vitro providing a possible link to PKD-mediated cytokine down-regulation and immune suppression.

Measurement of IgG antibodies to Chlamydia trachomatis by commercial enzyme immunoassays and immunofluorescence in sera from pregnant women and patients with infertility, pelvic inflammatory disease, ectopic pregnancy, and laboratory diagnosed Chlamydia psittaci/Chlamydia pneumoniae infection.

BACKGROUND: Screening for Chlamydia trachomatis specific antibodies is valuable in diagnosing asymptomatic pelvic inflammatory disease (PID) and tubal damage following repeated episodes of PID. The assays in current use are unsuitable for screening large numbers of samples so there is a need to develop more suitable assays. AIMS: To compare the performance of several commercial C trachomatis enzyme immunoassays (EIAs) (SeroCT, C tracho(pep), Medac p-EIA, Vircell and Labsystems C trachomatis IgG EIAs) using major outer membrane protein (MOMP), an inactivated organism EIA (Genzyme Virotech EIA), and a genus specific EIA (Platelia Chlamydia IgG) with the whole cell inclusion immunofluorescence (WIF) assay. In addition, to adapt, using time resolved fluorescence technology, the assay showing the highest correlation with WIF. METHODS: Ninety sera from patients presenting with ectopic pregnancies, 187 sera from those with a variety of types of infertility, 33 sera from cases of PID where a fourfold rise in WIF titre occurred, and 90 sera from antenatal clinic attenders were tested. A panel of 36 sera from laboratory diagnosed cases of Chlamydia psittaci/Chlamydia pneumoniae infection was also tested. RESULTS: The Genzyme Virotech EIA showed the highest rank correlation coefficient (0.82) with WIF, particularly at high WIF titres. The MOMP specific assays varied in their correlation with WIF, with rank correlation coefficients ranging from 0.70 (Medac p-EIA) to 0.80 (Vircell EIA). The Genzyme Virotech assay showed poor specificity (5.6%; 95% confidence interval (CI), 0.68% to 18.7%)--it was reactive with 34 of the panel of 36 C psittaci/C pneumoniae positive sera. The MOMP based EIAs showed high specificity, particularly the Medac p-ELISA (97.2%; 95% CI, 85.5% to 99.9%)--only one serum was reactive. In view of the good correlation between WIF and the Genzyme Virotech EIA, a time resolved fluorescence immunoassay (TRFIA) was developed using the Genzyme Virotech antigen. Using an appropriate cut off the TRFIA assay showed excellent correlation with WIF. CONCLUSIONS: The TRFIA assay may be useful as a screening assay, possibly in conjunction with one of the highly specific EIAs studied (for example, Medac p-EIA) to confirm the antibody specificity of sera selected by the screening assay.

Time resolved fluorometric immunoassay, using europium labelled antihuman IgG, for the detection of human tetanus antitoxin in serum.

A time resolved fluorometric immunoassay (TRFIA) has been developed and compared with an in house enzyme linked immunosorbent assay (ELISA) and commercial ELISA (Bindazyme) for the detection of tetanus antitoxin in human sera. A panel of 132 sera submitted for routine testing was used. Scatterplots showed a high degree of correlation between all three assays, although some divergence of results was apparent for low titre sera when comparing in house ELISA results with Bindazyme ELISA and TRFIA results. The TRFIA appeared to be more sensitive than the in house ELISA, and the Bindazyme assay compared well with the TRFIA. The intra-assay precision of all three assays, in terms of percentage coefficient of variation (%CV), was between 2.0% and 4.0%. The interassay precision ranged from 5% to 8% for the in house ELISA, 13% to 19% for the Bindazyme assay, and 11% to 13% for TRFIA. Both Bindazyme and TRFIA assays were simple to perform, accurate, reproducible, and amenable to automation. A particular benefit of the TRFIA was its large dynamic range, enabling tetanus antitoxin values of 0.01 IU/ml to 50 IU/ml to be measured with just one dilution of serum. TRFIA appears to be a useful serological technique worthy of further development.

Spatio-temporal genetic variability in the schistosome intermediate host Biomphalaria pfeifferi.

Genetic variation among temporally and spatially defined populations of the schistosome intermediate host, Biomphalaria pfeifferi, was analysed using RAPD markers. Snails were collected from each of eight sites that were 1-20 km apart, on four occasions, over a 2-year period. High levels of genetic variability were found within populations, between populations and between collections. Spatial differentiation and intrapopulation similarity were highest during periods of drought and were lowest following heavy rainfall. Temporal variability, consistent with founder effects, genetic drift and/or differential parasite pressure, was of similar or greater magnitude to that of the spatial variability. The results not only demonstrate that spatio-temporal sampling is necessary to understand the population genetics of short-lived species in variable habitats, but also provide empirical data to support current theoretical models of host-parasite co-evolution.