Assuntos
Infecções por Actinobacillus/veterinária , Actinobacillus pleuropneumoniae/classificação , Actinobacillus pleuropneumoniae/imunologia , Anticorpos Antibacterianos/sangue , Doenças dos Suínos/epidemiologia , Infecções por Actinobacillus/epidemiologia , Infecções por Actinobacillus/microbiologia , Animais , Inglaterra/epidemiologia , Feminino , Masculino , Estudos Soroepidemiológicos , Sorotipagem/veterinária , Suínos , Doenças dos Suínos/microbiologia , País de Gales/epidemiologiaRESUMO
Serotypes 3 and 8 of Actinobacillus pleuropneumoniae, the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the UK. Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross-reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3-specific pcr, based on the capsule locus, which can be used in a multiplex format with the organism's specific gene apxIV. The pcr test was evaluated on 266 strains of A pleuropneumoniae and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the UK.
Assuntos
Actinobacillus pleuropneumoniae/classificação , Actinobacillus pleuropneumoniae/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Sorotipagem/veterinária , Infecções por Actinobacillus/microbiologia , Infecções por Actinobacillus/veterinária , Animais , Suínos , Doenças dos Suínos/microbiologiaRESUMO
We describe a highly sensitive and specific multiplex PCR, based on capsular loci and the species specific apxIV gene, that unequivocally differentiates serovar 3, 6, and 8 Actinobacillus pleuropneumoniae strains that are cross-reactive in conventional immunological tests.