RESUMO
Saccharomyces cerevisiae is increasingly recognized clinically, and repeated isolations from patients on a hematology unit in Turku, Finland, led to an epidemiologic investigation. Isolates were recovered from multiple body sites of 23 patients (n = 180) from 1994 to 1995 and from 29 patients (n = 45) from 1997 to 2002; these plus 2 from the hospital kitchen were identified as S. cerevisiae. Isolates were genotyped by restriction fragment length polymorphism (RFLP) of genomic DNA after EcoR1 digestion. Of 108 isolates, 97 (95 patient isolates and 2 from the hospital kitchen) were DNA group B and identical in RFLP pattern. The remaining 11 isolates were DNA group A; 2 patients that shared a room had identical group A isolates, both converted to DNA group B type colonization within 2 months. In almost all patients, S. cerevisiae was first recovered after admission. These data suggest an endemic source of colonizing organisms, possibly from the hospital food preparation area.
Assuntos
Técnicas de Tipagem Micológica , Micoses/epidemiologia , Micoses/microbiologia , Saccharomyces cerevisiae/classificação , Saccharomyces cerevisiae/genética , Adolescente , Adulto , Idoso , Análise por Conglomerados , Impressões Digitais de DNA , DNA Fúngico/genética , DNA Fúngico/metabolismo , Desoxirribonuclease EcoRI/metabolismo , Feminino , Finlândia/epidemiologia , Genótipo , Humanos , Hospedeiro Imunocomprometido , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Polimorfismo de Fragmento de Restrição , Saccharomyces cerevisiae/isolamento & purificação , Adulto JovemRESUMO
Candida albicans and C. dubliniensis genotype differences among Israeli ethnic groups were assessed. Isolates from Jews (51), Arabs (35) and Druze (25) were genotyped. The distributions among ethnic groups were not different, however they differed (p = 0.002) from global populations. Therefore, C. albicans and C. dubliniensis genotype distribution differences in Israel are related to changes in all ethnic groups.
