RESUMO
Screening for densoviruses (DNVs) from Aedes, Culex and Toxorhynchites mosquitoes collected in Bangkok and surrounding regions identified two clades of Aedes DNV; Ae. aegypti DNV (AaeDNV) and Ae. albopictus DNV (AalDNV) by PCR-restriction fragment length polymorphism (PCR-RFLP). From nucleotide sequencing and phylogenetic analysis of PCR amplicons of a fragment of DNV capsid gene, these DNVs were shown to be new DNV genetic variations similar to AaeDNV. Isolation and identification of densoviruses from indigenous field mosquitoes reside in natural habitat should be helpful in monitoring the distribution of DNVs in important mosquitoes, especially Ae. aegypti and Ae. albopictus, vector for dengue and yellow fever viruses.
Assuntos
Aedes/virologia , Culex/virologia , DNA Viral/análise , Densovirus/genética , Variação Genética , Animais , Sequência de Bases , Culicidae/virologia , Filogenia , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , TailândiaRESUMO
Two clades of Aedes densovirus, Aedes aegypti densovirus and Aedes albopictus densovirus, were classified according to the origin of isolation. These two densoviruses were isolated from indigenous mosquitoes and mosquito cell lines, respectively. This group of invertebrate viruses belongs to the subfamily Densovirinae of the Parvoviridae family and infects only insects. Several types of densoviruses have been isolated from mosquitoes especially Aedes aegypti and Aedes albopictus, which are important vectors of dengue hemorrhagic fever and yellow fever in humans. We describe applications of post-PCR techniques, re- striction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP) to classify these two clades of Aedes densoviruses isolated from different origins. These methods are simple and rapid and are applicable to identify other groups of densoviruses isolated from biological samples.