RESUMO
The possible influence of phytochrome on the activity state of nitrate reductase (NR) was investigated in etiolated plants where the expression of the NR gene is known to be under the control of phytochrome. Activity state is defined as NR activity assayed in the presence of Mg(2+) as percentage of NR activity measured in the absence of Mg(2+). This measurement is assumed to reflect non-phosphorylated NR as percentage of total NR. Beside etiolated barley and maize leaves, a photosynthetic mutant of Lemna aequinoctialis was investigated and compared with the wild type. The increase of NR activity following a red light pulse, mediated via phytochrome, was confirmed in all etiolated plant species investigated as well as in both strains of L. aequinoctialis cultivated in glucose-containing medium. The effect of continuous red light surpassed the effect of a single red light pulse in each case. However, the results did not show any stimulating effect of phytochrome on the activity state caused by post-translational modulation. The activity state was strongly increased by continuous red light in the wild type of L. aequinoctialis but not in the photosynthetic mutant. These results show that the phytochrome system is not important for the post-translational regulation of NR.