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1.
Polymers (Basel) ; 16(6)2024 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-38543379

RESUMO

The response and mechanism of polyimide aerogel under electron irradiations were investigated. The experimental results indicated that electron irradiation could not damage the skeleton polyimide in the aerogel due to its high stability, but could result in a discharge within. The morphology of the discharge shows some dendritic discharge patterns, and the material surrounding the discharge channels was carbonized. The numerical simulation results indicated that the incident electrons, and also large amount induced secondary electrons, would be deposited inhomogeneously within the nano-porous polyimide aerogel. This would result in forming an ultra-high electrical potential of up to about 8.5 × 1010 V/m (which is far higher than the breakdown strength (2 × 108 V/m) of bulk polyimide materials) in a local region. This may be the leading cause of the obvious discharge in the materials. Furthermore, it was found that the actual reason for the discharge is related to the residual gas within the nano-porous structure; namely, the more internal residual gas (as a shorter-time vacuum pumping in the irradiated chamber), the more serious the discharge phenomenon. Correspondingly, the phenomenon may largely consist of both residual-gas discharge and surface flashover due to ultra-high local potentials induced by unevenly deposited charges in the porous aerogel.

2.
Nurs Open ; 10(12): 7713-7724, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37775963

RESUMO

AIMS: To translate and validate the Publication Pressure Questionnaire-revised (PPQ-r) among the Chinese nurses. DESIGN: A cross-sectional descriptive study. METHODS: The classical forward/backward translation of the PPQ-r was conducted first, and then the psychometric values were tested using the data collected in September, 2021 among 924 Chinese nurses from a university hospital in China by calculating its content validity index, construct validity, both convergent and discriminant validity, and internal consistency reliability following the COSMIN guidelines. RESULTS: The proposed three-factor structure of original PPQ-r cannot be confirmed in present study. With exploratory factor analysis, a new two-factor structure with 9 items was extracted, accounting for 62.718% of the variance. Confirmatory factor analysis indicated satisfactory goodness of all fit index values: χ2 = 75.53; df = 23; χ2 /df = 3.28; CFI = 0.973; TLI = 0.958; RMSEA = 0.07 and SRMR = 0.047. Both sub-scales yielded good internal consistency with Cronbach's alpha being 0.845 and 0.876, respectively. The Chinese version of PPQ-r could be accepted as a reliable and valid tool to measure the Chinese nurses' publication pressure. PATIENT OR PUBLIC CONTRIBUTION: Participation of nurses in this study was limited to the data provided through participant survey.


Assuntos
Tradução , Humanos , Estudos Transversais , Reprodutibilidade dos Testes , Inquéritos e Questionários , Hospitais Universitários
3.
Opt Express ; 31(19): 30092-30107, 2023 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-37710559

RESUMO

Multicolor two-photon endomicroscopy has become a highly competitive tool for functional imaging in biomedical researches. However, to make the imaging system miniature and applicable for freely behaving animal brain activity, metalenses have received much attention in compact imaging systems. For high resolution multicolor imaging and maximizing fluorescence collection, there is a challenge metalenses faced to achieve large numerical aperture (NA) and focus the NIR excitation and VIS emission lights of multiple fluorophores to the same distance simultaneously because of the limitation of the group delay range of the meta-units. In this paper, we proposed a high NA and polarization-insensitive ultra-broadband achromatic metalens specifically for achromatically focusing the excitation and emission light of multiple fluorophores commonly used in neuroscience studies. TiO2 and Si meta-unit libraries composed of heights, widths and the corresponding phase and group delay were constructed, and the optimal meta-units were selected by particle swarm optimization algorithm to engineer the dispersion of metalens in the VIS band and NIR band, respectively. Combining dispersion engineering with spatial multiplexing, the proposed metalens achieved the maximal effective NA up to 0.8 and large achromatic bandwidth ranging from 500 nm to 1050 nm, which exhibited the coefficient of variation of focal lengths was only 3.41%. The proposed achromatic metalens could successfully achromatically focus different fluorescence with any polarization, which was suitable for most fluorophores. Our results firmly establish that the proposed metalens can open the door to high resolution and minimally invasive multicolor two-photon functional imaging in intravital deep brain.

4.
Reprod Health ; 20(1): 62, 2023 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-37085786

RESUMO

BACKGROUND: Fertility intentions have been proved to be a reliable predictor of actual fertility behaviour. Also, childbirth-related fear (CBRF) has been proven to be negatively associated with childbirth readiness and fertility intentions among women, while childbirth readiness was positively related to fertility intentions. However, the associations and potential mechanisms between CBRF, childbirth readiness, and fertility intentions remain unknown. This study aimed to investigate the unique association between CBRF, childbirth readiness, and fertility intentions and whether childbirth readiness would mediate the relationship between CBRF and fertility intentions. METHOD: A cross-sectional study of women (N = 1119, aged 16-53 years) who gave birth within 72 h was conducted. Using a convenience sampling, women were recruited from obstetric wards-10 comprehensive hospitals and 3 specialized hospitals in 7 provinces in mainland China. Pearson correlation was used to examine the relationship between CBRF, childbirth readiness, fertility intentions, and social support. Multivariate linear regression was further used to analyze the association between demographic and personal characteristics, CBRF, childbirth readiness, and fertility intentions. Mediation analysis was used to examine whether childbirth readiness mediates the relationship between CBRF and fertility intentions. RESULTS: Women with high childbirth readiness (ß = 0.09, P = 0.002) had higher fertility intentions. However, women with high CBRF (ß = - 0.17, P < 0.001) were more likely to have lower fertility intentions. CBRF had both direct and indirect effects on the level of fertility intentions. As predicted, childbirth readiness mediated the relationship between CBRF and the level of fertility intentions (estimate = - 0.012, 95% bootstrap CI: - 0.021 to - 0.005). Higher CBRF was associated with lower scores of childbirth readiness, which was associated with lower levels of fertility intentions. CONCLUSIONS: This study established the evidence that CBRF had both direct and indirect effects on the level of fertility intentions and childbirth readiness mediated the relationship between CBRF and the level of fertility intentions. Specifically, higher CBRF was associated with lower scores of childbirth readiness, which was associated with lower levels of fertility intentions. This finding suggested that it is important for health policymakers and health providers to pay more attention to improving women's childbirth readiness, which might reduce the negative influence of CBRF on fertility intentions, thus strengthening their fertility intentions.


Over the past two decades, fertility rates have been dropping steadily in most countries. Fertility intentions have been proven to be a reliable predictor of actual fertility behaviour. The worldwide sluggish fertility trend has brought about a series of issues, such as accelerated population ageing, shrinking workforce, and economic decline. China has the largest national population in the world, accounting for nearly one-fifth of the world's population, thus having a huge impact on global population trends. Therefore, in the context of significant fertility decline and population policy changes, timely research on fertility intention is of great significance for China and other low-fertility countries. CBRF has been proven to be negatively associated with childbirth readiness and fertility intentions among women, while childbirth readiness was positively related to fertility intentions. This study aimed to investigate the unique association between fertility intentions, CBRF, and childbirth readiness, and whether childbirth readiness would mediate the relationship between CBRF and fertility intentions. In this cross-sectional study, we found that women with high childbirth readiness had higher fertility intentions. However, women with high CBRF were more likely to have lower fertility intentions. Furthermore, CBRF had both direct and indirect effects on the level of fertility intentions. Childbirth readiness mediated the relationship between CBRF and the level of fertility intentions. Higher CBRF was associated with lower scores of childbirth readiness, which was associated with lower levels of fertility intentions. This finding suggested that it is important for health policymakers and health providers to pay more attention to improving women's childbirth readiness, which might reduce the negative influence of CBRF on fertility intentions, thus strengthening their fertility intentions.


Assuntos
Intenção , Parto , Gravidez , Feminino , Humanos , Estudos Transversais , Fertilidade , Medo
5.
Front Public Health ; 11: 1108603, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36908408

RESUMO

Background and aim: Nurses' professional quality of life (ProQOL) is closely related to nursing life, and it is essential to clarify the professional quality of life of nurses and enhance it effectively. We aimed to explore the influence of family care and organizational support on the professional quality of life of clinical nurses and to improve the theoretical basis for improving the professional quality of life of clinical nurses in China. Methods: A single center, descriptive, cross-sectional design was used in this study. From February to April 2,022, 2,200 nurses from Tongji Hospital were selected as the study population, using the General Information Form, Family Care Scale, Professional Quality of Life Scale, Nurses' Sense of Organizational Support Questionnaire, and Work-Family Conflict Scale, and analyzing the relationship between professional quality of life and family care and organizational support among clinical nurses for correlation analysis as well as multiple linear regression to determine the factors affecting participants' Factors affecting ProQOL. P < 0.05 values were considered statistically significant. Results: The Cronbach coefficients of the scales were generally above 0.8, with good validity. All nurses had empathic satisfaction, burnout, and secondary trauma scores of 28.74 ± 3.83, 30.82 ± 3.45 and 29.40 ± 3.69, respectively, and correlation analysis, as well as multiple linear regression results, showed that the three dimensions of nurses' professional quality of life were associated with family care and organizational support (P < 0.05). Conclusions: The professional quality of life of nurses deserves to draw focused attention. The degree of family care and organizational support are predictive of professional quality of life, and nursing managers should pay attention to rationalizing tasks, pay attention to the physical and mental health of nursing staff, and improve the quality of life of nurses.


Assuntos
Enfermeiras e Enfermeiros , Recursos Humanos de Enfermagem Hospitalar , Humanos , Qualidade de Vida , Estudos Transversais , Recursos Humanos de Enfermagem Hospitalar/psicologia , Satisfação no Emprego
6.
Polymers (Basel) ; 14(23)2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36501571

RESUMO

In this work, we studied the influence of the geometry and degree of filling of glass dispersed particles on the optical and mechanical properties of flexible high-transmission composites, based on thermoplastic polyurethane. Glass spheres, glass flake and milling glass fiber were used as fillers. Studies of mechanical properties have shown that the introduction of any filler leads to a decrease in tensile strength and an increase in the elastic modulus of the composite material, however, with the introduction of glass flakes and milling glass fiber, a significant increase in the yield strength of the material is observed. The optical properties of composites with glass spheres decrease exponentially with an increase in the volume fraction of the filler. With an increase in the concentration of glass flakes and milling glass fiber to 10 vol.%, a sharp decrease in transmission is observed. With a further increase in concentration, the orientation of the filler along the film occurs, due to which the transmission in the visible range increases to values close to those of a pure polymer.

7.
Polymers (Basel) ; 14(17)2022 Aug 29.
Artigo em Inglês | MEDLINE | ID: mdl-36080635

RESUMO

The transport behaviors of proton into nanoporous materials were investigated using different Monte Carlo simulation codes such as GEANT4, Deeper and SRIM. The results indicated that porous structure could enhance the proton scattering effects due to a higher specific surface area and more boundaries. The existence of voids can deepen and widen the proton distribution in the targets due to relatively lower apparent density. Thus, the incident protons would transport deeper and form a wider Bragg peak in the end of the range, as the target materials are in a higher porosity state and/or have a larger pore size. The existence of voids also causes the local inhomogeneity of proton/energy distribution in micro/nano scales. As compared, the commonly used SRIM code can only be used to estimate roughly the incident proton range in nanoporous materials, based on a homogeneous apparent density equivalence rule. Moreover, the estimated errors of the proton range tend to increase with the porosity. The Deeper code (designed for evaluation of radiation effects of nuclear materials) can be used to simulate the transport behaviors of protons or heavy ions in a real porous material with porosity smaller than 52.3% due to its modeling difficulty, while the GEANT4 code has shown advantages in that it is suitable and has been proven to simulate proton transportation in nanoporous materials with porosity in its full range of 0~100%. The GEANT4 simulation results are proved consistent with the experimental data, implying compatibility to deal with ion transportation into homogeneously nanoporous materials.

8.
EBioMedicine ; 40: 276-289, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30594556

RESUMO

BACKGROUND: Endometrial cancer is one of the most common gynecological malignancies and has exhibited an increasing incidence rate in recent years. Cancer stem cells (CSCs), which are responsible for tumor growth and chemoresistance, have been confirmed in endometrial cancer. However, it is still challenging to identify endometrial cancer stem cells to then target for therapy. METHODS: Flow cytometry was used to identify the endometrial cancer stem cells. Sphere formation assay, western blotting, qRT-PCR assay, cell viability assay, xenograft assay and immunohistochemistry staining analysis were utilized to evaluate the effect of SPARC-related modular calcium binding 2 (SMOC-2) on the cells proliferation and drug resistance. Cell viability assay, qRT-PCR assay, immunofluorescence staining, Co-IP assay and luciferase reporter gene assay were performed to explore the possible molecular mechanism by which SMOC-2 activates WNT/ß-catenin pathway. FINDINGS: We found the expression of SPARC-related modular calcium binding 2 (SMOC-2), a member of SPARC family, was higher in endometrial CSCs than that in non-CSCs. SMOC-2 was also more highly expressed in spheres than in monolayer cultures. The silencing of SMOC-2 suppressed cell sphere ability; reduced the expression of the stemness-associated genes SOX2, OCT4 and NANOG; and enhanced chemosensitivity in endometrial cancer cells. By co-culture IP assay, we demonstrated that SMOC-2 directly interacted with WNT receptors (Fzd6 and LRP6), enhanced ligand-receptor interaction with canonical WNT ligands (Wnt3a and Wnt10b), and finally, activated the WNT/ß-catenin pathway in endometrial cancer. SMOC-2 expression was closely correlated with CSC markers CD133 and CD44 expression in endometrial cancer tissue. INTERPRETATION: Taken together, we conclude that SMOC-2 might be a novel endometrial cancer stem cell signature gene and therapeutic target for endometrial cancer. FUND: National Natural Science Foundation of China, Scientific and Technological Innovation Act Program of Shanghai Science and Technology Commission, Scientific and Technological Innovation Act Program of Fengxian Science and Technology Commission, Natural Science Foundation of Shanghai.


Assuntos
Proteínas de Ligação ao Cálcio/genética , Resistencia a Medicamentos Antineoplásicos/genética , Neoplasias do Endométrio/genética , Células-Tronco Neoplásicas/efeitos dos fármacos , Células-Tronco Neoplásicas/metabolismo , Animais , Biomarcadores , Linhagem Celular Tumoral , Sobrevivência Celular/genética , Modelos Animais de Doenças , Neoplasias do Endométrio/metabolismo , Neoplasias do Endométrio/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Genes Reporter , Humanos , Camundongos , Gradação de Tumores , Estadiamento de Neoplasias , Paclitaxel/farmacologia , Via de Sinalização Wnt/efeitos dos fármacos , Ensaios Antitumorais Modelo de Xenoenxerto
9.
Nanomaterials (Basel) ; 8(12)2018 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-30544631

RESUMO

The dual-mode emission and multicolor outputs in the time domain from core-shell microcrystals are presented. The core-shell microcrystals, with NaYF4:Yb/Er as the core and NaYF4:Ce/Tb/Eu as the shell, were successfully fabricated by employing the hydrothermal method, which confines the activator ions into a separate region and minimizes the effect of surface quenching. The material is capable of both upconversion and downshifting emission, and their multicolor outputs in response to 980 nm near-infrared (NIR) excitation laser and 252 nm, and 395 nm ultraviolet (UV) excitation light have been investigated. Furthermore, the tunable color emissions by controlling the Tb3+- Eu3+ ratio in shells and the energy transfer of Ce3+→Tb3+→ Eu3+ were discussed in details. In addition, color tuning of core-shell-structured microrods from green to red region in the time domain could be obtained by setting suitable delay time. Due to downshifting multicolor outputs (time-resolved and pump-wavelength-induced downshifting) coupled with the upconversion mode, the core-shell microrods can be potentially applied to displays and high-level security.

10.
Cancer Med ; 7(11): 5716-5726, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30353687

RESUMO

Lung adenocarcinoma (LUAD) is a common diagnosed disease with high-mortality rate, and its prognostic implications are under discovered. DNA methylation aberrations are not only an important event for dysregulation of gene expression during tumorigenesis but also a revolution in epigenetics by identifying key prognostic biomarkers for multiple cancers. In this study, we analyzed methylation status of 485 578 CpG sites and RNA-seq transcriptomes of 20 532 genes for 1095 LUAD samples in TCGA database. The association between DNA methylation and the prognostic value of the corresponding gene expression was identified as well. In total, ten aberrantly methylated and dysregulated genes (AURKA, BLK, CNTN2, HMGA1, PTTG1, TNS4, DAPK2, MFSD2A, THSD1, and WNT7A) were highlighted which were significantly correlated with overall survival of 492 LUAD patients, which were all reported as tumor-associated genes in other various cancers and worthy of further investigated and might be used as therapeutic targets for LUAD. Together, methylation aberrances regulate gene expression level during tumorigenesis and influence prognosis of LUAD patients. Integrating knowledge of epigenetics and expression of genes can be useful for an in-depth understanding of cancer mechanism and for the eventual purpose of precisely prognostic and therapeutic target verification.


Assuntos
Adenocarcinoma de Pulmão/genética , Biomarcadores Tumorais/genética , Metilação de DNA , Perfilação da Expressão Gênica/métodos , Neoplasias Pulmonares/genética , Ilhas de CpG , Bases de Dados Genéticas , Epigênese Genética , Regulação Neoplásica da Expressão Gênica , Humanos , Prognóstico , Análise de Sequência de RNA , Análise de Sobrevida
11.
Phys Chem Chem Phys ; 20(14): 9516-9522, 2018 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-29570204

RESUMO

Recent years have witnessed the progress of lanthanide-doped materials from fundamental material synthesis to targeted practical applications such as optical applications in photodetection, anti-counterfeiting, volumetric display, optical communication, as well as biological imaging. The unique compositions and structures of well-designed lanthanide ion-doped materials could expand and strengthen their application performances. Herein, we report dual-mode luminescent crystalline microrods that spatially confine upconversion and downconversion photophysical process within defined regions using the specially designed heterogeneous structure. Through an epitaxial growth procedure, downconversion tips have been conjugated with the parent upconversion microrods in oriented directions. This spatially confined structure can effectively depress the deleterious energy depletion in lanthanide ions homogeneously doped materials, and as a result, the red, green, and blue upconversion intensities have been enhanced by 334, 225, and 22 times, respectively. Moreover, the induced tips hardly disturb the upconversion process of the microrod seeds. Upon 980 nm laser or ultraviolet lamp excitation, tunable emission colors were realized in the single tip-modified microrod, indicating potential applications of these microrods for high-level dual-mode anti-counterfeiting.

12.
Nanomaterials (Basel) ; 7(12)2017 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-29240698

RESUMO

We synthesized the uniform core-shell microstructured compounds with hexagonal phase NaYF4:Er/Yb microrods as the core and hexagonal phase NaLnF4 (NaYbF4, NaLuF4:Yb/Tm, NaYF4:Yb/Er, NaYF4:Eu) as the shell based on the hydrothermal reaction. These microscale core-shell structures provided a platform for the spatially confining optical process while possessing high luminescence efficiency. The thickness of the shell could be controlled by adjusting the amounts of shell precursor, which significantly affected the intensity of the shell dopant ions emission and the emission color of core-shell upconversion luminescence (UCL). The uniform NaYF4@NaLnF4 (Ln = Y, Lu, Yb) microrods, with a series of rare-earth ions doped into the core and shell layer at various doping concentrations, achieved color-tuning of the upconversion (UC) emission and dual-mode emission at the single-microcrystal level, thus allowing the efficient utilization of core-shell microcrystals in the photonics and security labeling. This study suggests a new class of luminescent materials in the microscopic field.

13.
Mater Sci Eng C Mater Biol Appl ; 81: 177-181, 2017 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-28887962

RESUMO

We developed a highly efficient optical thermometer based on intensity ratio of upconversion green fluorescence of Er3+/Yb3+-codoped NaYF4 microcrystals. The sensor consists simply of a 980nm laser diode, one narrow-band interference filter, two lenses, one Si-photocell and one multimeter, while being without use of spectrometer and additional electronics. The device not only has a simple, compact structure (hence a low cost), but also displays highly efficient sensing performance, characterized by large signal-to-noise ratio due to strong fluorescence intensity, high thermal resolution and sensitivity, which have the values 1.3K and 1.24×10-2K-1, respectively, at the physiological temperature 310K. The excellent sensing performance of the device was further confirmed by the results of the measurements repeated using a spectrometer. The thermometer is highly generalized that can be applied to other luminescent materials, and shows great potential for the physiological temperature sensing in biological tissues and cells.


Assuntos
Termômetros , Érbio , Fluorescência , Fluoretos , Luminescência , Ítrio
14.
Opt Express ; 24(18): 20062-8, 2016 Sep 05.
Artigo em Inglês | MEDLINE | ID: mdl-27607615

RESUMO

Here we propose a new method for trapping the resonant metallic particles with the 4π tight focusing (high numerical-aperture (NA)) system, which is illuminated by radial polarization light. Numerical simulations have indicated the maximum total optical force is 16.1pN while with nearly zero scattering force under axis trapping, which keeps the gradient force predominant. Furthermore, the distribution of total force is centrosymmetric and odd. We also gain stable 3D trap with an equilibrium point along z axis and r axis as in normal optical tweezers. What's more, we obtain the nearly pure longitudinal field. The maximum transverse intensity is only 2.3 × 10-3 and the transverse spot size reaches 0.36λ, which is below Abbe's diffraction limit.

15.
J Cancer ; 7(11): 1481-6, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27471564

RESUMO

BACKGROUND: Radical hysterectomy with pelvic lymphadenectomy is the standard surgical treatment for patients with stage IA2-IB1 cervical cancer, but the wide excision increases the complications. OBJECTIVE: To analyze the feasibility of narrowing resection of parametrial tissues in stage IA2-IB1 cervical cancer. STUDY DESIGN: Retrospectively analyzed the pathological and clinical data of patients with stage IA2-IB1 cervical cancer who received radical hysterectomy with pelvic lymphadenectomy in OB/GYN Hospital, Fudan University, China from Jan 2008 to Dec 2011. The affected factors of parametrial metastases and outcomes were discussed. The single factor analysis was made with χ2 test, and the relationship of the resection width of parametrial tissues and the patients' outcomes was analyzed with χ2 test and log-rank. P-values <0.05 were considered statistically significant. RESULTS: There were 31 cases recurred, 26 cases died of cervical cancer in 513 patients during the follow-up period (from 2 months to 66 months, averaged 39 months). The low-risk factors included diameter of tumor ≤2cm, depth of cervical myometrial invasion<1/2 and without lymph vascular involvement. There were no parametrial metastases in cases with all three low-risk factors. Whether the resection width of parametrial tissues ≥3cm or not had no statistically significant effect on progression free survival (PFS) or overall survival (OS) of low-risk patients. D2-40 and CD31 were related with parametrial metastases, but not with recurrence or outcomes. CONCLUSIONS: The resection width of parametrial tissues has no effect on PFS and OS of low-risk patients, and narrowing resection of parametrial tissues (<3cm) is feasible.

16.
Carbohydr Polym ; 142: 222-9, 2016 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-26917394

RESUMO

Porous poly(L-lactic acid) (PLLA) sheets were prepared by uniaxial stretching PLLA sheets containing starch filler. Here, the starch filler content, stretching ratio, stretching rate and stretching temperature are important factors to influence the structure of the porous PLLA sheets, therefore, they have been investigated in detail. The pore size distribution and tortuosity were characterized by Mercury Intrusion Porosimetry. The results revealed that the porosity and pore size enlarged with the increase of the starch filler content and stretching ratio, while shrank with the rise of stretching temperature. On the other hand, the pore structure almost had no changes with the stretching rate ranging between 5 and 40 mm/min. In order to test and verify that the porous PLLA sheet was suitable for the tissue engineering, the starch particles were removed by selective enzymatic degradation and its in vitro biocompatibility to osteoblast-like MC3T3-E1 cells was investigated.


Assuntos
Materiais Biocompatíveis/química , Poliésteres/química , Amido/química , Animais , Linhagem Celular , Camundongos , Osteoblastos/citologia , Porosidade , Engenharia Tecidual
17.
J Opt Soc Am A Opt Image Sci Vis ; 32(8): 1576-80, 2015 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-26367303

RESUMO

We investigate three-dimensional focus shaping generated from double-mode cylindrical vector beams with the Gaussian and Bessel-Gaussian pupil apodization functions by choosing the suitable polarization states of beams. Further, we compare them with that generated from the Laguerre-Gaussian pupil apodization function in the same situation. We find that the focus shaping generated from the Gaussian beam has the smallest zero intensity spot size. However, the situation of the Bessel-Gaussian beam not only possesses stability, which makes it suitable when applied in optical trapping, but also shows the best uniformity, which indicates its excellent performance in super-resolution fluorescence microscopy.

18.
Int J Biol Macromol ; 76: 49-57, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25709010

RESUMO

Biodegradable polymer composites based on poly(ɛ-caprolactone) (PCL) and poly(lactic acid) (PLA) fibers with diameters of 18, 26, 180 µm were prepared by melt compounding. The PLA fiber content in the composites was constant at 20% by weight. The effects of fibers with different diameters on the physical properties and enzymatic degradation of PCL were investigated. The morphological analysis indicated good interfacial adhesion between PCL and PLA fiber, which was beneficial to improve the physical properties of PCL. With increasing PLA fiber diameter, the complex viscosity and modulus of PCL were significantly increased, especially at low frequencies, indicating that the hindered effect of the fiber on the mobility of the PCL molecular chains was more obvious when PLA fiber diameter was thicker. However, as for the mechanical properties, the reinforcement was more obvious to PCL with the smaller PLA fiber diameter. This was because increasing efficient load transfer may be appeared due to the larger surface area and better interface bonding force of the fiber with thinner diameters. The enzymatic degradation of PCL was accelerated with the addition of large PLA fiber diameter of 26 and 180 µm, and hardly changed with the small PLA fiber diameter of 18 µm.


Assuntos
Caproatos/química , Ácido Láctico/química , Lactonas/química , Polímeros/química , Materiais Biocompatíveis/química , Teste de Materiais , Poliésteres , Reologia , Termodinâmica
19.
Int J Biol Macromol ; 67: 343-50, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24704167

RESUMO

Biodegradable and biosourced poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) fiber was used as a reinforcing agent, and environment friendly poly(ɛ-caprolactone) (PCL) composites were prepared by melt compounding. The mechanical properties, rheological properties, and enzymatic degradation of the PCL composites were investigated in detail. With the addition of PHBV fibers, the PCL composites showed increased tensile yielding strength and modulus. Especially, the storage modulus from the results of dynamic mechanical analysis was increased significantly, suggesting that PCL was obviously reinforced by adding PHBV fibers. With increasing the PHBV fiber content, the complex viscosity and modulus of PCL increased, especially at low frequencies, indicating that a network structure was formed in the composites. The network structure resulted in evident solid-like response due to the restriction of the chain mobility of PCL matrix, which was further confirmed by the Han and Cole-Cole plots. The morphology, evaluated by scanning electron microscopy, indicated PCL and PHBV fiber were not highly incompatible and the interfacial adhesion was good, which was beneficial to the reinforcement effect. The biodegradability of the PCL was significantly promoted after composites preparation. Such studies are of great interest in the development of environment friendly composites from biodegradable polymers.


Assuntos
Plásticos Biodegradáveis/química , Poliésteres/química , Polímeros/química , Materiais Biocompatíveis/química , Microscopia Eletrônica de Varredura
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