Veterinary antibiotics differ in phytotoxicity on oilseed rape grown over a wide range of concentrations.

Residues of veterinary antibiotics are a worldwide problem of increasing concern due to their persistence and diverse negative effects on organisms, including crops, and limited understanding of their phytotoxicity. Therefore, this study aimed to compare the phytotoxic effects of veterinary antibiotics tetracycline (TC) and ciprofloxacin (CIP) applied in a wide range of concentrations on model plant oilseed rape (Brassica napus). Overall phytotoxicity of 1-500 mg kg-1 of TC and CIP was investigated based on morphological, biochemical, and physiological plant response. Photosystem II (PSII) performance was suppressed by TC even under environmentally relevant concentration (1 mg kg-1), with an increasing effect proportionally to TC concentration in soil. In contrast, CIP was found to be more phytotoxic than TC when applied at high concentrations, inducing a powerful oxidative burst, impairment of photosynthetic performance, collapse of antioxidative protection and sugar metabolism, and in turn, complete growth retardation at 250 and 500 mg kg-1 CIP treatments. Results of our study suggest that TC and CIP pollution do not pose a significant risk to oilseed rapes in many little anthropogenically affected agro-environments where TC or CIP concentrations do not exceed 1 mg kg-1; however, intensive application of manure with high CIP concentrations (more than 50 mg kg-1) might be detrimental to plants and, in turn, lead to diminished agricultural production and a potential risk to human health.

Effects of elevated CO2 concentration and temperature on the mixed-culture grown wild mustard (Sinapis arvensis L.) response to auxin herbicide.

Recently, there has been growing concern over the potential impact of CO2 concentration and temperature on herbicide efficacy. The aim of the study was to examine the influence of single elevated CO2 (400 vs. 800 ppm) and elevated CO2 in combination with temperature (21 °C vs. 25 °C) on the effects of auxin herbicide 4-chloro-2-methylphenoxyacetic acid (MCPA) (0.5-2 × field recommended rate) to wild mustard (Sinapis arvensis L.) grown in mixed-culture with spring barley (Hordeum vulgare L.). MCPA had a detrimental effect on aboveground and belowground biomass, content of chlorophylls, enzymatic and non-enzymatic antioxidants and induced oxidative stress. The significant decline in photosynthetic rate, stomatal conductance and transpiration with MCPA dose was detected. Elevated CO2 reinforced MCPA efficacy on S. arvensis: sharper decline in biomass, photosynthetic rate and antioxidant enzymes and more pronounced lipid peroxidation were detected. Under elevated CO2 and temperature, MCPA efficacy to control S. arvensis dropped due to herbicide dilution because of increased root:shoot ratio, higher activity of antioxidants and less pronounced oxidative damage. Reinforced MCPA impact on weeds under elevated CO2 resulted in higher H. vulgare biomass, while decreased MCPA efficacy under elevated CO2 and temperature reduced H. vulgare biomass.

Association between El Niño-Southern Oscillation events and stroke: a case-crossover study in Kaunas city, Lithuania, 2000-2015.

The aim of this study was to determine the association between the daily number of cases of ischemic stroke (IS) and hemorrhagic stroke (HS) in patients aged 25-64 years and the El Niño-Southern Oscillation (ENSO) events during 2000-2015. As an indicator of the effect of the ENSO, the monthly NIÑO 3.4 index (Equatorial Pacific Sea Surface Temperature) was used. During the 5844-day study period, 5600 cases of stroke (3170 (56.61%) in men and 2430 (43.39%) in women) were analyzed. Of these, 4354 (77.8%) cases were IS, and 1041 (18.6%) cases were HS. In 3496 (62.2%) cases, stroke occurred in the age group of 55-64 years. In the analysis, we used the following categories of the ENSO events: strong La Niña, moderate La Niña, moderate El Niño, and strong El Niño. The effect of the ENSO was examined by using the multivariate Poisson regression adjusting for weather variables. The highest risk of both strokes (BS) was observed on days of strong and moderate La Niña (rate ratio (RR) 1.27, 95% CI 1.13-1.42) and RR = 1.15 (1.07-1.23), respectively), while the risk for IS was the highest on days of moderate El Niño (RR = 1.11(1.02-1.20)). A lower risk for BS was found on days of strong El Niño (RR = 0.77(0.62-0.97)). We found that ENSO events affected the occurrence of BS and IS in all age groups, and the strongest effect was observed among females. The results of this study provide new evidence that ENSO events may affect the risk of stroke, especially the risk of IS.

O3 pollution in a future climate increases the competition between summer rape and wild mustard.

The initial aim of this study was to evaluate an effect of elevated CO2 concentration and air temperature (future climate) and O3 pollution on mono- and mixed-culture grown summer rape (Brassica napus L.) and wild mustard (Sinapis arvensis L.). The second task was to reveal the mechanisms of the shift in plants' competitiveness in response to single and combined environmental changes. Plants were grown in mono- and mixed-cultures under current climate (CC) (400 µmol mol-1 of CO2, 21/14 °C day/night temperature) or future climate (FC) conditions (800 µmol mol-1 of CO2, 25/18 °C day/night temperature) with and without O3 treatment (180 µg m-3). Competition had relatively little effect on growth of both species at current climate, independent of O3 treatment. In contrast, competitive effect of both plant species considerably increased under FC, and especially FC + O3 conditions, when growth of mixed-culture rape reduced up to 48% and that of wild mustard up to 80%. The mechanisms of elevated competitiveness of rape under the future climate consisted of better antioxidative protection, particularly elevated total antioxidative capacity and activities of peroxidase and ascorbate peroxidase. Whereas stronger oxidative damage, disproportionally high activities of H2O2 scavenging enzymes and lower pool of soluble sugars in mixed-culture wild mustard reduced its competitiveness under FC + O3 conditions. In conclusion it must be pointed out, that regardless improved competitive abilities of rape under FC and FC + O3 conditions, competition with wild mustard reduced growth, indicating increased weed-induced yield losses in the future climate, especially with concomitant intensification of O3 pollution.