The uterusmasculinus of the Eurasian beaver (Castor fever L.) - The appraisal of fast hormone regulation by membrane androgen and estrogen receptors involvement.

The phenomenon of remaining paramesonephric ducts (uterus masculinus) in males of some animal species concerning its role is still an unresolved issue. Now it is well-recognized that sex hormonal regulation of reproductive physiology involves also fast nongenomic control of cellular processes through noncanonical signaling. Herein, in the uterus masculinus of Eurasian beaver membrane androgen receptor (metal ion transporter Zrt- and Irt-like protein 9; ZIP9) and membrane estrogen receptor (G protein-coupled estrogen receptor; GPER) were studied. Scanning electron microscopy together with anatomical analysis revealed that Eurasian male beavers possess one double uterus (uterus duplex). Two odd parts open into the vagina but do not form a common lumen. The length of the horns is the most differential feature of this organ in studied animals. Uterus masculinus is not a tightly closed tubular structure. Histological analysis showed an analogy to the female uterus structure however no glands but gland-like structures were observed. The presence and abundant localization of ZIP9 and GPER proteins in cells of uterus masculinus was confirmed by immunohistochemistry while their expression was measured by western blotting. GPER expression in remnants was lower (P < 0.001) than those in the female uterus. Parallelly, the concentration of progesterone and estradiol but not testosterone was lower (P < 0.05 and P < 0.01, respectively) in comparison to the female uterus. Our study, for the first time, reports the involvement of fast hormonal regulation in the uterus masculinus of Eurasian beavers reflecting the participation of this organ in the creation local hormonal environment. Moreover, the uterus masculinus seems to be a useful research model for understanding and resolving urgent biological problems such as gender identities and having children by women with a lack of uterus or anatomical barriers on this level.

Overview of diagnostic performance and results for the first operation phase in Wendelstein 7-X (invited).

Wendelstein 7-X, a superconducting optimized stellarator built in Greifswald/Germany, started its first plasmas with the last closed flux surface (LCFS) defined by 5 uncooled graphite limiters in December 2015. At the end of the 10 weeks long experimental campaign (OP1.1) more than 20 independent diagnostic systems were in operation, allowing detailed studies of many interesting plasma phenomena. For example, fast neutral gas manometers supported by video cameras (including one fast-frame camera with frame rates of tens of kHz) as well as visible cameras with different interference filters, with field of views covering all ten half-modules of the stellarator, discovered a MARFE-like radiation zone on the inboard side of machine module 4. This structure is presumably triggered by an inadvertent plasma-wall interaction in module 4 resulting in a high impurity influx that terminates some discharges by radiation cooling. The main plasma parameters achieved in OP1.1 exceeded predicted values in discharges of a length reaching 6 s. Although OP1.1 is characterized by short pulses, many of the diagnostics are already designed for quasi-steady state operation of 30 min discharges heated at 10 MW of ECRH. An overview of diagnostic performance for OP1.1 is given, including some highlights from the physics campaigns.

Dielectric study of interaction of water with normal and osteoarthritis femoral condyle cartilage.

The main goal of this paper is the in vitro study of healthy and osteoarthritis (OA) human cartilage using the dielectric spectroscopy in the alpha-dispersion region of the electric field and in the temperatures from 25 to 140°C. The activation energy of conductivity needed to break the bonds formed by water in the extracellular matrix takes the average values of 61kJ/mol and 44kJ/mol for the control and OA cartilages, respectively. At 28°C, the small difference appears in the permittivity decrement between the control and OA cartilages, while the conductivity increment is about 2 times higher for the control tissue than that for the OA tissue. At 75°C, the conductivity increment for both of these samples is 8 times higher than their respective permittivity decrement. In addition, at 140°C the values of these both parameters for the OA tissue decrease by 8 times as compared to those recorded for the control sample. The relaxation frequency of about 10kHz is similar for both of these samples. The knowledge on dielectric properties of healthy and OA cartilage may prove relevant to tissue engineering focused on the repair of cartilage lesions via the layered structure designing.

Status of the diagnostics development for the first operation phase of the stellarator Wendelstein 7-X.

An overview of the diagnostics which are essential for the first operational phase of Wendelstein 7-X and the set of diagnostics expected to be ready for operation at this time are presented. The ongoing investigations of how to cope with high levels of stray Electron Cyclotron Resonance Heating (ECRH) radiation in the ultraviolet (UV)/visible/infrared (IR) optical diagnostics are described.

Use of personalized ventilation for improving health, comfort, and performance at high room temperature and humidity.

UNLABELLED: The effect of personalized ventilation (PV) on people's health, comfort, and performance in a warm and humid environment (26 and 28°C at 70% relative humidity) was studied and compared with their responses in a comfortable environment (23°C and 40% relative humidity). Thirty subjects participated in five 4-h experiments in a climate chamber. Under the conditions with PV, the subjects were able to control the rate and direction of the supplied personalized flow of clean air. Subjective responses were collected through questionnaires. During all exposures, the subjects were occupied with tasks used to assess their performance. Objective measures of tear film stability, concentration of stress biomarkers in saliva, and eye blinking rate were taken. Using PV significantly improved the perceived air quality (PAQ) and thermal sensation and decreased the intensity of Sick Building Syndrome (SBS) symptoms to those prevailing in a comfortable room environment without PV. Self-estimated and objectively measured performance was improved. Increasing the temperature and relative humidity, but not the use of PV, significantly decreased tear film quality and the concentration of salivary alpha-amylase, indicating lower mental arousal and alertness. The use of PV improved tear film stability as compared to that in a warm environment without PV. PRACTICAL IMPLICATIONS: In practice, the supply of clean, cool, and less humid air by PV at each workstation will make it possible to raise room temperatures above the upper comfortable limit suggested in the present standards without adversely affecting the occupants' health [Sick Building Syndrome (SBS) symptoms], comfort (thermal and perceived air quality), and performance. This may lead to energy savings.

Increased expression of TGF-beta/Smad proteins in basal cell carcinoma.

BACKGROUND: Basal cell carcinoma (BCC) is the most common cancer in humans placing a significant burden on healthcare services worldwide. There is an increasing evidence that the development of cutaneous epithelial tumours is pathogenetically linked to dysregulations of the transforming growth factor beta (TGF-beta) and its signalling molecules, the Smads. OBJECTIVE: In the present study we aimed to investigate the mRNA as well as protein expression of TGF-beta/ Smad signalling proteins in patients with BCC and healthy controls. METHODS: In this prospective pilot study, 24 patients with BCC were recruited. Punch biopsies were harvested from the centre of the tumour (lesional) as well as an adjacent healthy skin site (non-lesional controls). In addition to the specimens of BCC patients, skin samples (healthy controls) were obtained from subjects who had no history of skin cancer (n = 25). Real-time RT-PCR as well as immunohistochemistry was performed. - RESULTS: The mRNA levels of TGF-b/Smad transducers observed in healthy controls did not significantly differ from TGF-beta/Smad levels observed in non-lesional skin of BCCs patients (P > 0.05). RT-PCR revealed significant mRNA overexpression of TGF-beta1, Smad3, and Smad7 in BCCs as compared to non-lesional skin (P < 0.05). TGF-beta1 mRNA expression significantly correlated with Smad3 (r = 0.60; P < 0.05) and Smad7 (r = 0.76; P < 0.05) levels. Immunohistochemistry demonstrated marked protein overexpression of Smad3 in tumour tissue as compared to non-lesional skin. CONCLUSIONS: Our data suggest a possible role of TGF-beta/Smad signalling in the pathogenesis of BCC.

Measurement and prediction of indoor air quality using a breathing thermal manikin.

UNLABELLED: The analyses performed in this paper reveal that a breathing thermal manikin with realistic simulation of respiration including breathing cycle, pulmonary ventilation rate, frequency and breathing mode, gas concentration, humidity and temperature of exhaled air and human body shape and surface temperature is sensitive enough to perform reliable measurement of characteristics of air as inhaled by occupants. The temperature, humidity, and pollution concentration in the inhaled air can be measured accurately with a thermal manikin without breathing simulation if they are measured at the upper lip at a distance of <0.01 m from the face. Body surface temperature, shape and posture as well as clothing insulation have impact on the measured inhaled air parameters. Proper simulation of breathing, especially of exhalation, is needed for studying the transport of exhaled air between occupants. A method for predicting air acceptability based on inhaled air parameters and known exposure-response relationships established in experiments with human subjects is suggested. PRACTICAL IMPLICATIONS: Recommendations for optimal simulation of human breathing by means of a breathing thermal manikin when studying pollution concentration, temperature and humidity of the inhaled air as well as the transport of exhaled air (which may carry infectious agents) between occupants are outlined. In order to compare results obtained with breathing thermal manikins, their nose and mouth geometry should be standardized.

Human response to personalized ventilation and mixing ventilation.

UNLABELLED: The response of 60 human subjects to a personalized ventilation system (PVS), providing control of positioning of the air terminal device and the airflow rate, was compared with the response to mixing ventilation (MV). Perceived air quality, thermal comfort, intensity of Sick Building Syndrome symptoms and performance of subjects were studied during 3 h 45 min exposures. In case of MV alone the room air temperature was 23 degrees C and 26 degrees C. The PVS supplied outdoor air at 23 degrees C or 20 degrees C or recirculated room air at 23 degrees C when the room temperature was 23 degrees C, and outdoor air at 20 degrees C when the room temperature was 26 degrees C. The PVS providing outdoor air improved perceived air quality and decreased SBS symptoms compared to MV alone and when the room air was re-circulated through the PVS. The percentage dissatisfied with air quality, 3 min after initial occupancy, decreased from 22% with MV to 7% with PVS; and from 49% to 20%, at room temperatures 23 degrees C and 26 degrees C, respectively. Over time, these differences in percentage dissatisfied decreased markedly. Headache and decreased ability to think clearly were reported as least intense when the PVS supplied outdoor air at 20 degrees C, while the most intense symptoms occurred with MV. PVS increased self-estimated performance. PRACTICAL IMPLICATIONS: Personalized ventilation can improve occupants' thermal comfort, perceived air quality and decrease the intensity of SBS symptoms compared to mixing ventilation. Occupants will use the provided individual control of airflow rate and positioning of the air terminal device to obtain preferred microenvironment in rooms where the air temperature is within the range recommended by indoor climate standards. Development of more efficient air terminal devices is recommended.

Oxazolidinones mechanism of action: inhibition of the first peptide bond formation.

Oxazolidinones are potent inhibitors of bacterial protein biosynthesis. Previous studies have demonstrated that this new class of antimicrobial agent blocks translation by inhibiting initiation complex formation, while post-initiation translation by polysomes and poly(U)-dependent translation is not a target for these compounds. We found that oxazolidinones inhibit translation of natural mRNA templates but have no significant effect on poly(A)-dependent translation. Here we show that various oxazolidinones inhibit ribosomal peptidyltransferase activity in the simple reaction of 70 S ribosomes using initiator-tRNA or N-protected CCA-Phe as a P-site substrate and puromycin as an A-site substrate. Steady-state kinetic analysis shows that oxazolidinones display a competitive inhibition pattern with respect to both the P-site and A-site substrates. This is consistent with a rapid equilibrium, ordered mechanism of the peptidyltransferase reaction, wherein binding of the A-site substrate can occur only after complex formation between peptidyltransferase and the P-site substrate. We propose that oxazolidinones inhibit bacterial protein biosynthesis by interfering with the binding of initiator fMet-tRNA(i)(Met) to the ribosomal peptidyltransferase P-site, which is vacant only prior to the formation of the first peptide bond.

Complementary and alternative medicine: herbal therapies for diabetes.

Interest in and use of complementary and alternative medicine have increased in recent years, with as much as 40% of the general population having used some form of complementary and alternative medicine in the past year. Although recent national surveys have not adequately assessed the use of complementary and alternative medicine in minority populations, its use in many minority populations is at least equal to and possibly greater than that in the general population. One condition for which minority populations are likely to use complementary and alternative medicine therapies is diabetes, which is particularly prevalent in Mexican-American and Native-American communities. Many minority cultures have a long history of using herbal preparations to treat diabetes, and recent research suggests that some herbal therapies may have a role in the treatment of this complex disease. However, many questions remain regarding the proper use of herbal therapies for diabetes, particularly in regard to dosage and contaminants. Therefore, physicians who work with minority populations should ask patients about their use of these therapies and must become knowledgeable about their safety and efficacy.

[Terminology of scoliosis]. / Terminologia skoliozy.

The most important entries concerning scoliosis have been presented in an alphabetical order. Some definitions, better describing three-planar scoliosis deformity, suggested by Scoliosis Research Society have been included.

[Early results of surgical treatment for congenital hip dislocation in children using Degi's method in children under 18 months of age]. / Wczesne wyniki leczenia operacyjnego wrodzonego zwichniecia stawu biodrowego metoda degi u dzieci ponizej 18 miesiaca zycia.

Early results of surgical treatment of congential hip dislocation by the Dega method in 18 children (20 hips) aged from 6 to 17 months are presented. Following the excision of the soft tissue obstructions and Dega transiliac osteotomy stable reduction of the hip has been achieved in all cases. Proximal femoral osteotomy has been performed in 6 cases only. Clinical results were markedly superior over radiological ones. Fair radiological outcomes resulted from surgical errors or avascular necrosis of the femoral head due to the previous conservative treatment. Avascular necrosis after operative treatment has not been noted.

[Treatment of idiopathic scoliosis with Texas Scottish Rite Hospital instrumentation (TSRH)--preliminary report]. / Leczenie operacyjne skoliozy idiopatycznej przy uzyciu instrumentarium Texas Scottish Rite Hospital--doniesienie wstepne.

General principles of construction of TSRH instrumentation and its use for treatment of idiopathic King type III scoliosis have been presented. Surgical technique has been described on the basis of procedure performed in the case of 13 years old girl with primary right sided thoracic adolescent scoliosis.

Microscopic picture of the vesicular glands in young boars with respect to feeding level.

On 27 German Landrace boars studies were conducted to describe the effect of feeding level during the growth period on the microscopic picture of vesicular glands in boars at 5, 6 and 7 months of age. Investigations have shown that differentiation of feeding level during the growth period affected the development of internal structures of the parenchyma in the boars' vesicular glands. The size of the tubules, their number within the field of vision and height of glandular epithelium lining the tubules were the highest in the group of animals fed at increased level.

Significance of membrane thickness in the sonographic evaluation of twin gestations.

A retrospective study of 55 twin pregnancies was performed to determine the role of sonography in distinguishing between dichorionic and monochorionic diamniotic gestations solely by evaluating the thickness of the membrane between the fetuses. The presence of a thick dividing membrane indicated a dichorionic diamniotic gestation in 38 (90%) of 42 cases in which it was identified. Inability to identify a membrane between dichorionic diamniotic twins occurred most commonly during the third trimester. A thin membrane indicated monochorionic diamniotic twinning and was seen in three (25%) of 12 cases. Membrane thickness was indeterminate in one case. Therefore, on the basis of the thickness of the dividing membrane imaged by sonography, dichorionic diamniotic gestations can be distinguished from monochorionic diamniotic gestations. Inability to show a separating membrane by sonography, however, can occur with any form of twin gestation, particularly in the third trimester. Membrane thickness should be used in conjunction with other sonographic criteria to predict the amnionicity and chorionicity of twin gestations.