Assuntos
Bronquiectasia , Bronquiolite , Bronquite Crônica , Fibrose Cística , Antibacterianos/uso terapêutico , Água Corporal/metabolismo , Bronquiectasia/tratamento farmacológico , Bronquiectasia/etiologia , Bronquiolite/tratamento farmacológico , Bronquiolite/etiologia , Bronquite Crônica/tratamento farmacológico , Bronquite Crônica/etiologia , Fibrose Cística/etiologia , Fibrose Cística/microbiologia , Humanos , Macrolídeos/uso terapêutico , Muco/metabolismo , Infiltração de Neutrófilos/imunologia , Infecções por Pseudomonas , Pseudomonas aeruginosa/patogenicidade , Pseudomonas aeruginosa/fisiologia , Percepção de Quorum , Sistema Respiratório/imunologia , Sistema Respiratório/metabolismo , Sistema Respiratório/microbiologia , Linfócitos T/imunologiaRESUMO
Of the non-physiological compounds in glucose-rich peritoneal dialysis fluid, we investigated the synergistic cytotoxicity of acidity and 3,4-Dideoxyglucosone-3-ene(3,4-DGE) under the existence of lactate using human peritoneal mesothelial cells (HPMC). The effect of pH on cell viability at various levels of pH (5.5, 6.7, 7.15), with or without lactate was examined by adding 1N-HCl to phosphate buffer solution. We also examined the cytotoxic effects of 3,4-DGE and pH (5.5, 6.7 or 7.15). Additionally, we compared the cytotoxic effects of 3,4-DGE and pH (5.5, 6.7 or 7.15) under existence of lactate (40 meq/L) or absence of lactate. The cells were exposed to these solutions for 2 or 4 h. Cell viability was determined by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenylterazolium bromide) assay. 3,4-DGE or acidic solution alone had no significant effects on MTT viability under the absence of lactate. However, acidic solutions containing 3,4-DGE significantly decreased MTT viability under the existence of lactate. The MTT viability of HPMC was not decreased by 3,4-DGE or acidity alone under the absence of lactate. However, the combination of acidity and 3,4-DGE markedly decreased MTT viability under the existence of lactate, strongly suggesting the synergistic cytotoxicity of 3,4-DGE and acidity under the existence of lactate.
Assuntos
Ácido Láctico/farmacologia , Pironas/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Soluções para Diálise/química , Soluções para Diálise/farmacologia , Sinergismo Farmacológico , Células Epiteliais/efeitos dos fármacos , Glucose/farmacologia , Humanos , Concentração de Íons de Hidrogênio , Diálise Peritoneal , Peritônio/citologia , Peritônio/efeitos dos fármacos , Fatores de TempoRESUMO
Of the nonphysiological compounds in glucose-rich peritoneal dialysate, we investigated the cytotoxicity and synergistic cytotoxicity of acidity and glucose degradation products (GDPs) using human peritoneal mesothelial cells (HPMC). The effect of pH on cell viability was examined by adding 1N HCl to a phosphate-buffered solution (pH > or = 5.5). We also examined the cytotoxic effects of various GDPs [glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosone (3DG), alone or in combination] and pH (5.5 or 6.7). The cells were exposed to these solutions for 2 or 4 h. Cell viability was determined by 3,(4,5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay. Although the MTT viability of HPMC was not decreased by GDP or acidity alone, the combination of acidity and GDP markedly decreased MTT viability, strongly suggesting the synergistic cytotoxicity of GDP and acidity.
