RESUMO
A survey of sera containing antibodies to multiple low-incidence antigens revealed a variety of patterns of reactions with NFLD+, BOW+, and Wu+ red cell samples. Although NFLD, BOW, and Wu are distinct antigenic determinants (International Society of Blood Transfusion numbers 700.37, 700.46, and 700.13, respectively), their ability to absorb and elute "crossreactive" antibodies indicates a serologic and possibly a genetic relationship among the three.
Assuntos
Antígenos de Grupos Sanguíneos/imunologia , Antígenos de Grupos Sanguíneos/genética , Reações Cruzadas , Humanos , Isoantígenos/imunologia , FenótipoRESUMO
Concordance of reactions of Wu+ and 'Hov'+ cells with 153 sera containing multiple specificities to low-incidence antigens indicates that the 'two' antigens are identical. This conclusion is confirmed by absorption and elution studies.
Assuntos
Antígenos/sangue , Absorção , Especificidade de Anticorpos/imunologia , HumanosRESUMO
DNA samples from families informative for the Diego (DI), Dombrock (DO) and Yt (YT) blood group loci were analyzed with a cDNA probe defining a Taq I polymorphism at the glycophorin C locus (GYPC). Recombination between GYPC and DI, DO and YT occurs. Hence GYPC is differentiated from all established blood group system loci.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Glicoforinas/genética , Alelos , Southern Blotting , DNA/análise , Genótipo , Humanos , Linhagem , Polimorfismo de Fragmento de RestriçãoRESUMO
Studies of 91 individuals in three families allowed a genetic-linkage analysis of the gene governing the production of the low-incidence red cell antigen Wra and provided evidence that Wra is not a member of the Scianna, Landsteiner-Wiener, Chido/Rodgers, or XK blood group systems, and that the "WR" locus is excluded from autosomal sites or regions 1p34-p22.1, 1p21-q23, 1q32, 2p25, 3q21, 4q28-q32, 6p24-q12, 9q34.1-q34.2, 13q14.1-q14.2, 14q24.3-q32.1, 14q32.33, 16p13, 16q22.1, and 21q21-q22.1. "WR" is also excluded from within specified genetic distances of chromosomes 8 (GPT), 18 (JK), 19 (C3), 20 (ADA), and 22 (P1) loci, which brings its exclusion to approximately 10 percent (320cM) of the total genetic map of the genome. The possibility that "WR" is pseudoautosomal is deemed to be highly unlikely.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Isoantígenos/genética , Mapeamento Cromossômico , Feminino , Humanos , Escore Lod , Masculino , Recombinação GenéticaRESUMO
Family studies have provided the final piece of evidence for the assignment of Auberger to the Lutheran blood group system. Lods derived from combined paternal and maternal meioses (zeta = 10.83 at theta = 0.00) strongly support the contention that a single gene controls the expression of both Au and LU antigens. Consequently, the International Society of Blood Transfusion Working Party on Terminology has designated Aua and Aub as LU18 and LU19, respectively.
Assuntos
Antígenos/genética , Antígenos de Grupos Sanguíneos/genética , Sistema do Grupo Sanguíneo Lutheran/genética , Membrana Eritrocítica/imunologia , Humanos , Meiose , Polimorfismo Genético , Recombinação GenéticaRESUMO
In an attempt to assign the Colton blood group locus (CO) we have successfully revisited chromosome 7. CO is linked to the argininosuccinate synthetase pseudogene 11 locus (ASSP11) with z = 5.79 at theta = 0.07 for combined paternal and maternal meioses. We propose a 7p position for CO.
Assuntos
Argininossuccinato Sintase/metabolismo , Antígenos de Grupos Sanguíneos/genética , Cromossomos Humanos Par 7 , Ligases/metabolismo , Pseudogenes , Mapeamento Cromossômico , Sondas de DNA , Genes , Humanos , Escore Lod , Linhagem , Recombinação GenéticaRESUMO
DNA from a series of families segregating for Aub was analyzed with a genomic DNA probe which defines a Bg1 I polymorphism for apolipoprotein C II (APOC2). The investigation revealed that the gene for Aub is closely linked to APOC2 (z = 8.43 at theta = 0.00) for paternal and maternal meioses combined, to LW (z = 3.61 at theta = 0.00) in paternal meioses and less closely linked to SE (z = 3.10 at theta = 0.09) for combined paternal and maternal meioses. Therefore, we propose a chromosome 19 location for the Aub gene.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Cromossomos Humanos Par 19 , Ligação Genética , Mapeamento Cromossômico , HumanosRESUMO
A case of mild hemolytic disease of the newborn is presented which was caused by an antibody to a hitherto unknown antigen of low incidence. This antigen, now designated as HOFM (ISBT number 700050) was detected in 6 relatives, and in all of them, it was associated with an unusually weakened expression of C antigen. The serological data indicate that HOFM may be part of the Rh system, but the genetic data, although supportive of this interpretation, are inconclusive.
Assuntos
Eritroblastose Fetal/imunologia , Eritrócitos/imunologia , Isoantígenos/análise , Sistema do Grupo Sanguíneo Rh-Hr/imunologia , Adulto , Especificidade de Anticorpos/imunologia , Feminino , Humanos , Recém-Nascido , Masculino , LinhagemRESUMO
Peak lods (zeta) of 3.48 at an estimated recombination fraction (theta) of 0.28 derived from 63 male and 90 female meioses indicate linkage between the KEL and YT blood group loci. Consideration is given to two families; a realistic interpretation of the data increases zeta to 4.24 at theta = 0.26.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Ligação Genética , Sistema do Grupo Sanguíneo de Kell/genética , Feminino , Marcadores Genéticos/sangue , Humanos , Escore Lod , Masculino , Fenótipo , Recombinação Genética , Fatores SexuaisRESUMO
The ability of Candida albicans IFO 1385 to adhere to acrylic and the partial characterization of an adhesive substance, named AS, which was isolated from the yeast, were studied in vitro. The results obtained were as follows: 1. The cells cultured in the synthetic media (YNB) containing 500 mM galactose showed a much greater tendency to adhere than did those cells cultured in the YNB containing 500 mM glucose. 2. More cells prepared by the standing cultivation adhered to acrylic than did those prepared by the stirring cultivation. 3. A large number of the adherent cells was obtained when the acrylic plates were incubated at 37 degrees C for 90 min in the cell suspension at a concentration of 1.0 x 10(7) cells/ml. The plates were observed without staining. 4. AS was isolated from the surface of C. albicans, grown on different carbon sources (50 mM glucose, 500 mM glucose and 500 mM galactose), by treatment with ultrasonication. 5. Three different kinds of AS isolated from the three carbon sources were slightly soluble in distilled water. All were similar in composition to each other, and contained 62-68% carbohydrate (as glucose) and 23-26% protein (as BSA). 6. Silica particles adhered to acrylic coated with AS and pretreatment of acrylic with AS promoted C. albicans adhesion. However, similar pretreatment inhibited subsequent Candida glabrata and Candida krusei adhesion. As to subsequent adhesion of Candida tropicalis, no significant data were obtained. 7. Adhesion assay using the silica particles, the adhesive ability of the AS was significantly reduced by treatment with trypsin or pronase E, but not with papain, alpha-amylase, dextranase or zymolyase.
Assuntos
Resinas Acrílicas , Candida albicans , Adesão Celular , Propriedades de SuperfícieRESUMO
Genetic linkage analyses of the blood group system loci CO, DI, DO, KEL and YT in relation to F13B indicate that these loci are not members of the RCA gene cluster on chromosome 1q32. The data are presented to finalize the exclusion of the DAF carried red cell antigens from all of the 17 established blood group systems.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Cromossomos Humanos Par 1 , Ativação do Complemento/genética , Ligação Genética , Escore Lod , Proteínas de Membrana/genética , Antígenos CD55 , Humanos , Família MultigênicaRESUMO
Accumulated family information was compiled in an attempt to verify the chromosomal location of the Colton blood group locus (CO). Two-point linkage analysis of CO and 46 other polymorphic loci excludes CO from 1p36 to 1q23, 3q21 to 3q26, 4q13 to 4q28, 6p24 to 6 cen, and 19p13.2 to 19 cen and from linkage groups bounded by ABO and ORM, PI and IGHG, and HP and GOT2. The dwindling odds of linkage between CO:JK are reinforced (z = -5.28 at theta = 0.20). Close linkage of CO with ACP1 and D2S5 could not be demonstrated. The proposed chromosome 2 location of CO is therefore questioned.
Assuntos
Mapeamento Cromossômico , Cromossomos Humanos Par 2 , Genes , Ligação Genética , Marcadores Genéticos , HumanosRESUMO
Data are presented on 30 high and low incidence antigens and on the distribution of the alleles of 14 blood group systems in a random sample of Newfoundlanders. The distribution of alleles in Newfoundland was compared to founder populations where possible and to the Canadian Caucasian population in general; no significant differences were found. Six rare alleles were observed (IN*a, NFLD, TAR, RH*x, RH*w, RH*V) in a population of 234 individuals. A high incidence of rare alleles has been reported in other isolate populations.
Assuntos
Antígenos de Grupos Sanguíneos/genética , Frequência do Gene , Alelos , Consanguinidade , Humanos , Terra Nova e Labrador , Linhagem , Fenótipo , Polimorfismo Genético , População Branca/genéticaRESUMO
The results of the present study provide independent support for F13A:HLA linkage and refine the F13A:HLA and F13A:GLO1 linkage relationships. Analysis of the corresponding recombination fractions for the total paternal F13A:HLA and F13A:GLO1 peak lod scores (z) indicates a locus order of 6pter:F13A:HLA:GLO1:cen. Lod scores between F13A and PLG, a locus recently assigned to chromosome 6, exclude close linkage between these loci.
Assuntos
Cromossomos Humanos Par 6 , Fator XIII/genética , Genes , Ligação Genética , Antígenos HLA/genética , Lactoilglutationa Liase/genética , Liases/genética , Complexo Principal de Histocompatibilidade , Criança , Feminino , Humanos , Masculino , TransglutaminasesRESUMO
The data establish linkage in both sexes for LDLR:LW (zeta = 8.43 at theta = 0.00) and in the male for LDLR:LU (zeta = 3.31 at theta = 0.00) and for LW:APOC2 (zeta = 3.90 at theta = 0.00). They confirm LDLR:C3 and APOC2:LU linkage in both sexes, and LW:LU linkage in the male. The loci constitute two tightly linked gene clusters, LDLR, C3, LW and APOC2, LU, SE, distinguished by measurable linkage in female meioses within but not between clusters. Argument is supported for a 19p13.2-cen position for LW and a long arm position for LU and SE.
Assuntos
Cromossomos Humanos Par 19 , Ligação Genética , Apolipoproteína C-II , Apolipoproteínas C/genética , Complemento C3/genética , Feminino , Humanos , Imunoglobulina A Secretora/genética , Escore Lod , Sistema do Grupo Sanguíneo Lutheran/genética , Masculino , Polimorfismo de Fragmento de Restrição , Receptores de LDL/genética , Sistema do Grupo Sanguíneo Rh-Hr/genéticaRESUMO
The low-frequency red cell antigen NFLD was identified in 2 Japanese donors. A family study showed that the antigen is not part of the P1 blood group system. Anti-NFLD was found in serum of several donors (frequency of 0.044%).
