RESUMO
BACKGROUND: The triad "insulin resistance, prediabetes, diabetes" is three independent neologies with characteristic features and development. In addition, each are characterized by progression and the possibility of transition from one form to other. Due to the fact that diabetes is one of the common diseases associated with high rates of disability, it is necessary to improve diagnostic methods and educational regimens for successful prevention and treatment of the disease. OBJECTIVE: We investigated Band 3 protein (B3p) level, osmotic resistance of erythrocytes, the total antioxidant activity (TAA) of blood serum, level of HbA1 in group patients with insulin resistance (IR), prediabetes, and Type 2 Diabetes Mellitus (T2DM) and comparative with health control group. METHODS: We used original, accurate research methods that measure the essence of the studied quantities. RESULTS: Disruptions of glucose and insulin homeostasis ay lead to the initiation of oxidative stress (in our study demonstrated by a decrease of TAA of blood serum) increased redox-sensitive PTP activity and aberrant band 3 phosphorylation, potentially leading to reduced erythrocyte deformability. At the same time glycation of Hb during T2DM may affect its cross-link with membrane proteins, in particular with B3p, and although appears to limit its cross-linking and decrease its clusterization ability, induces alterations in the cytoskeletal matrix, and thereby decrease erythrocytes' osmotic resistance making them more susceptible to hemolysis. CONCLUSIONS: The osmotic resistance of the erythrocytes can be used as a sensitive marker for the detection of the early stages of hyperglycemia (prediabetes). This set of clinical trials will make it possible to identify diseases that make up the triad at an early stage. Early detection of disorders and continued research in this direction will help in the development of a diagnostic scheme for the prevention of such patients. Based on our data, research into anti-oxidation drugs is very important. With the help of the array of studies described in the article and antioxidant treatment, the likelihood of successful treatment will increase.
RESUMO
The aim of the study was to establish the dependence of memory processes and learning ability in gamma-irradiated white mice on the age and period after irradiation. The 3-month and 1-year-old male mice (Mus musculus) were used in the study. Mice whole-body irradiation was performed at a dose of 5 Gy with 137Cs by using a "Gamma-capsule-2". Spatial learning and formation of memory were estimated in the elevated-type multi-way maze and elevated plus-maze. Experiments were carried out 48 hours and 30 days after irradiation for seven days (five trials each day). The number of errors (deviations from optimal trajectory) and total time for crossing the maze were calculated. The results of the study indicate that ionizing irradiation with a total dose of 5 Gy results in a delayed spatial learning process, causes spatial memory and behavior changes in different age groups of animals - aged mice turned out to be more radio-resistant. Age-related radio-resistance plays an especially major role in the early stage of post-radiation recovery. Though, the late radiation aging effect is especially pronounced in young animals.
