Intra-gastric string test: an effective tool for diagnosing tuberculosis in adults unable to produce sputum.

SETTING: Tuberculosis (TB) diagnosis is challenging in patients who are unable to produce sputum. The string test, a method for retrieving enteropathogens, is a potential alternative diagnostic tool. OBJECTIVES: To compare the TB detection yield and tolerability of the string test and that of sputum induction in adults with presumed TB in Uganda. DESIGN: Cross-sectional study. String test and sputum induction were performed consecutively in patients unable to produce sputum. The string was removed after a 2-h intra-gastric downtime. Sputum induction used nebulised 5% saline for 20 min. Light-emitting diode fluorescence microscopy, Löwenstein-Jensen and MGIT culture were performed on all specimens, and the Xpert(®) MTB/RIF assay on a subset. Tolerability questionnaires were administered. RESULTS: Of 210 patients included in the study, 59% were human immunodeficiency virus (HIV) positive and 50 (23.8%) were diagnosed with TB. Of these, 48 (96.0%) were detected with the string test and 46 (92.0%) with sputum induction. In patients with specimens collected using both methods for paired analysis, the yield of microscopy detection with the string test was 13.8% (26/188) vs. 13.3% (25/188) with sputum induction (P = 1.0). The yield increased to 22.9% (42/183) using culture for string test vs. 24.6% (45/183) for sputum induction (P = 0.37). Xpert detected TB in 15/96 (15.6%) patients with the string test vs. 17/96 (17.7%) with sputum induction (P = 0.62). Tolerability was comparable. CONCLUSION: The string test was well tolerated and provided similar yields to sputum induction, offering a viable alternative in resource-limited settings with minimal risk of transmission.

[Malignant tropical malaria in native Africans living in a large city]. / Zlokachestvennaia tropicheskaia maliariia u korennykh afrikantsev, zhitelei krupnogo goroda.

The clinical and laboratory features of severe Plasmodium falciparum-induced malaria were analyzed in 91 adult patients living a large African city. Within a week, 52 patients developed the disease from the manifestations of overall intoxication to the complete picture of severe malaria accompanied by coma. Fifty eight patients were found to be residents of Conakry and 54 of them left the city 2 months before the malaria attack. Eighty one patients had experienced malaria, including 38 patients had 1-2 attacks in the past year. The patients were parenterally treated with quinine in a dose of 750-850 mg of the active ingredient for 24 hours during 4.1 +/- 1.7 days at hospital. In 17 of 34 patients, parasitemia disappeared from single to 5-10 parasites and more in the field of thick drop field, in the other 17 patients it decreased from 5-10 to single parasites at recovery. Twenty four comatose patients died at days 3-8 of hospital stay, most of them had symptoms of oligoanuria. The high cost of hospitalization and specific drugs were the reasons for late referral to hospital and for the use of low daily and course doses of quinine. The necessity of reviewing the principal trends of a national malaria control programme.