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1.
Oncogene ; 23(19): 3487-94, 2004 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-15007382

RESUMO

We have constructed a high-resolution genomic microarray of human chromosome 16q, and used it for comparative genomic hybridization analysis of 16 prostate tumors. We demarcated 10 regions of genomic loss between 16q23.1 and 16qter that occurred in five or more samples. Mining expression array data from four independent studies allowed us to identify 11 genes that were frequently underexpressed in prostate cancer and that co-localized with a region of genomic loss. Quantitative expression analyses of these genes in matched tumor and benign tissue from 13 patients showed that six of these 11 (WWOX, WFDC1, MAF, FOXF1, MVD and the predicted novel transcript Q9H0B8 (NM_031476)) had significant and consistent downregulation in the tumors relative to normal prostate tissue expression making them candidate tumor suppressor genes.


Assuntos
Cromossomos Humanos Par 16 , Genes Supressores de Tumor , Hibridização de Ácido Nucleico , Neoplasias da Próstata/genética , Humanos , Masculino
2.
Am J Pathol ; 162(3): 763-70, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12598311

RESUMO

We have used prostate cancer, the most commonly diagnosed noncutaneous neoplasm among men, to investigate the feasibility of performing genomic array analyses of archival tissue. Prostate-specific antigen and a biopsy Gleason grade have not proven to be accurate in predicting clinical outcome, yet they remain the only accepted biomarkers for prostate cancer. It is likely that distinct spectra of genomic alterations underlie these phenotypic differences, and that once identified, may be used to differentiate between indolent and aggressive tumors. Array comparative genomic hybridization allows quantitative detection and mapping of copy number aberrations in tumors and subsequent associations to be made with clinical outcome. Archived tissues are needed to have patients with sufficient clinical follow-up. In this report, 20 formalin-fixed and paraffin-embedded prostate cancer samples originating from 1986 to 1996 were studied. We present a straightforward protocol and demonstrate the utility of archived tissue for array comparative genomic hybridization with a 2400 element BAC array that provides high-resolution detection of both deletions and amplifications.


Assuntos
Aberrações Cromossômicas , Análise de Sequência com Séries de Oligonucleotídeos , Neoplasias da Próstata/genética , Neoplasias da Próstata/patologia , Mapeamento Cromossômico , Formaldeído , Técnicas Histológicas , Humanos , Masculino , Metástase Neoplásica , Hibridização de Ácido Nucleico , Parafina , Sensibilidade e Especificidade , Resultado do Tratamento
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