Chronic lymphoplasmacytic villonodular proliferative synovitis in a 10-year-old Jack Russell Terrier dog.

We describe a case of chronic lymphoplasmacytic villonodular synovitis (CLPVNS) associated with cranial cruciate ligament (CCL) disease in a 10-year-old spayed Jack Russell Terrier bitch. The bitch was presented to a veterinary clinic with severe, non-weight bearing, acute left hindlimb lameness. The bitch had previously been treated surgically for stifle CCL disease of the same joint, using the lateral fabellar suture (LFS) technique. Since the treatment, the patient had a history of intermittent left hindlimb non-weight bearing lameness that was manageable with nonsteroidal anti-inflammatory drugs (NSAIDs). Palpation and manipulation of the affected stifle elicited severe pain. There were no other clinical or orthopaedic abnormalities. Orthogonal radiographs of the affected stifle revealed moderate degenerative joint disease and osteolytic lesions on the lateral aspect of the lateral femoral condyle and the head of the fibula. A fluid aspirate from this joint was negative for bacterial growth on culture. Cytology results were suspicious for CLPVNS. Exploratory arthrotomy, synovectomy, debridement and lavage of the affected joint were performed. Bone and synovial membrane biopsy samples of the joint were obtained and submitted to a laboratory for a histopathological confirmatory diagnosis. CLPVNS was tentatively diagnosed by cytology, and confirmed by histopathology of biopsy samples. This case report highlights the importance of checking for CLPVNS in dogs with lameness associated with CCL disease, as reports show it to be underreported or misdiagnosed.

Seroprevalence of African horse sickness in selected donkey populations in Namibia.

BACKGROUND AND AIM: African horse sickness (AHS) is a non-contagious viral disease of horses and other equids caused by an arbovirus belonging to the Reoviridae family and genus Orbivirus. AHS is an endemic disease that is responsible for the death of a high number of horses every year in Namibia. At present, there is no information on the prevalence and distribution of AHS virus (AHSV) serotypes in the different regions of Namibia. Therefore, this survey aimed to fill this knowledge gap by investigating the AHSV seroprevalence in Namibian donkeys. MATERIALS AND METHODS: A total of 260 blood samples (20 samples for each region) were randomly collected from donkeys aged between 3 and 5 years. Sera were screened for AHSV-specific immunoglobulin G antibodies using acommercial competitive enzyme-linked immunosorbent assay kit and samples positive to AHSV antibodies were further tested by serum neutralization (SN) assay to evaluate the AHSV serotype-specific immune response. RESULTS: Seroprevalence of antibodies against AHSV in Namibian donkeys was 63.5%. The AHSV prevalence was significantly higher in the northern region (64%) than in the southern region (36%). A significantly (p<0.05) higher number of donkeys had antibodies against AHSV-6 (37.8%) and AHSV-9 (37.8%). The AHSV-2, AHSV-6, and AHSV-9 prevalence were higher (p<0.05) in the northern regions compared to the southern regions. None of the donkeys in this study, however, tested positive for AHSV-8. CONCLUSION: Results of the current study indicate that all AHSV serotypes have either circulated previously or are circulating in Namibia except for AHSV-8. In particular, AHSV-1, -2, -3, -4, -5, -6, and -9 serotypes have circulated or are circulating in the northern region of Namibia, while AHSV-1, -4, -5, -6, -7, and -9 have infected donkeys in the south. AHSV-9 and AHSV-6 were the most prevalent serotypes detected in donkeys in this study. SN results showed that several donkeys from Kavango East, Kavango West, and Ohangwena regions had been exposed to multiple serotypes, indicating the possibility of cocirculation of several strains in Namibia.

A retrospective sero-epidemiological survey of bovine brucellosis on commercial and communal farming systems in Namibia from 2004 to 2018.

Cattle production is the major livestock production activity and the mainstay of Namibia's economy. Sustained beef exports are contingent on a sound sanitary environment where diseases such as brucellosis are under control. In this retrospective study, 49,718 bovine brucellosis testing results from 2004 to 2018 were analyzed to determine the proportion of sero-positive cattle and herds, and the spatial distribution of positive reactors from commercial and communal areas. In total, 244 positive reactors were identified based on the Rose Bengal Test (RBT) and the Complement Fixation Test (CFT) in series, giving an overall proportion of infected animals of 0.49% (244/49,718; 95% CI, 0.43-0.56%) and an overall proportion of infected herds of 9.26% (78/842; 95% CI, 7.49-11.41%). There was a higher proportion of sero-positive communal herds (33.09%) and cattle (10.27%) than commercial herds (4.67%) and cattle (0.24%; p < 0.05). Annually, the proportion of positive reactors was 0-1.37% in the commercial area and 0-52.38% in the communal areas, with a clear decline in positive reactors in the communal areas. Within the commercial sector, the proportion of positive reactor dairy, beef, and export cattle was 0.19% (51/27,067; 95% CI, 0.14-0.25%), 0.30% (48/16,098; 95% CI, 0.22-0.40%), and 0.33% (16/4811; 95% CI, 0.20-0.54%), respectively. Abortions were found to be the major reason for Brucella testing in the communal areas. About 12.65% (96/759) of abortion-linked sera tested positive in the communal areas, but none were positive in beef or dairy cattle. Widespread vaccination of cattle and robust planned surveillance is recommended to reduce the incidence of the disease, its associated production losses and public health risk.

Demographics, distribution, ownership and naming patterns of pets presented to a mobile clinic for sterilisation in Namibia.

This study analysed the demographics, spatial distribution, ownership and naming patterns of dogs and cats presented to the University of Namibia's veterinary mobile clinic for sterilisation from small underserved towns around Namibia. The proportional distribution of pets was determined based on species, sex, age, owner gender, town of origin and naming categories. Overall, 84.4% (n = 2909) of the animals presented for sterilisation were dogs and the remainder were cats (15.6%, n = 539). Of the dogs presented for sterilisation, 51.9% (n = 1509) were male and 48.1% (n = 1400) were female. In cats, 51.4% (n = 277) were male, whilst 48.6% (n = 262) were female. Overall, the majority of pets (68.2%) were presented for sterilisation from urban areas than rural areas (31.8%). About 49.8% of men and 24.2% of women that presented pets for sterilisation came from urban areas, whilst 20.1% of the women and 11.7% of the men that presented pets for sterilisation were from rural areas. Of all the pets presented for sterilisation, the majority were male-owned (64%, n = 2206). Pets were mainly presented for sterilisation at 2 years (41.1%), 2 to 4 years (32.4%) and 4 to 6 years (15.4%). The naming of pets was mainly after people (42.4%), circumstances (20.6%) and appearance (15.5%). This community engagement exercise yielded valuable demographic data indicating that pet origin, sex and species and owner gender were important factors in determining the voluntary presentation of pets for sterilisation in the study area.

Production performance of sheep and goat breeds at a farm in a semi-arid region of Namibia.

This study retrospectively investigated the effect of breed and season on the lambing/kidding dynamics, growth performance, neonatal viability, and weaning dynamics of sheep (Damara, Dorper, and Swakara) and goats (Boer goat and Kalahari Red) at a farm in the Khomas Region of Namibia between 2004 and 2015. Litter size was dependent on breed (X2(12, N = 3388) = 796, p < 0.001), with twinning more frequent in Dorper sheep and Kalahari Red and Boer goats than in the Damara and Swakara sheep (p < 0.05), while triplets were more prevalent in the Dorper sheep and Kalahari Red goats (2.8% and 1.0%, respectively; p < 0.05). Distribution of birth weight categories was dependent on breed. There was a significant difference in the proportions of birth weight categories between breeds (X2(12, N = 3388) = 467, p < 0.001) whereby Dorper lambs were mostly born weighing below 3 kg (2.6%, p < 0.05); Boer goat kids, Kalahari Red kids, and Damara lambs were mostly born weighing 3 to < 4 kg (4.3%, 6.3% and 19.9%, respectively; p < 0.05); Swakara lambs were mostly born weighing 4 to < 5 kg (12.2%, p < 0.05), and Swakara lambs were mostly born weighing ≥ 5 kg (3.3% and 2.3%, respectively, p < 0.05). Weaning age categories were dependent on breed (X2(12, N = 3388) = 241, p < 0.001) whereby Dorper lambs were mostly weaned at below 3 months of age (2.8%, p < 0.05); Damara lambs were mostly weaned at 3 to < 5 months of age (12%, p < 0.05), and Boer goat kids were mostly weaned at ≥ 5 months of age (0.9%, p < 0.05). Neonatal viability was dependent on breed (X2(8, N = 3388) = 49.2, p < 0.001) whereby Dorper lambs were more susceptible to abortions and neonatal deaths (0.6% and 1.5%, respectively; p < 0.05) than the rest of the breeds. Breed and lambing season interacted to produce effects on the birth weight of offspring although lambing season alone did not have a significant effect on Boer goat and Kalahari Red kids' birth weights.

A feather cyst causing vertebral bone lysis and spinal cord compression in a Lohmann Brown layer.

A feather cyst in the cervical region which, through complications of inward growth, resulted in compression of the cervical spinal cord of a Lohmann Brown layer is described. It is postulated that expansion of the cyst exerted pressure on the adjacent cervical vertebra and through bone lysis created an opening through which the cyst protruded, thereby exerting pressure on the spinal cord. The affected spinal cord segment was reduced to a fifth of its normal diameter. The bird most likely died of starvation because of limb and neck paralysis and disorientation. Although the cause of the feather cyst was not conclusively identified, moulting and trauma could have triggered its formation and subsequent growth.

Applicability of teeth examination as a tool for age estimation in a semi-arid cattle production environment in Namibia.

Regression analysis was used to evaluate teeth examination as a tool for estimating age in beef and dairy cattle at Neudamm farm, Namibia, in 2018. There was a significant difference in age (months) between the overall mean actual age (M=60, SD=4.57) and the mean estimated age (M=57, SD=4.52 months) in beef cattle; t(74) = 3.61, p<0.001. There was no significant difference between the overall mean estimated age and the mean actual age (M=42.5, SD=5.18) and (M=41.3, SD=5.21 months) in dairy cattle; t(30) = 1.45, p=0.16. There was a very strong positive relationship between the overall teeth examination-estimated age and actual age in beef cattle [r (74) = 0.98, p<0.001]. There was a very strong positive relationship between the overall teeth examination-estimated age and actual age in dairy cattle [r (30) = 0.99, p<0.001]. A very strong positive relationship was also evident between the teeth examination-estimated age and actual age in beef cattle aged 45 months and below [r (37) = 0.93, p<0.001]. There was a very strong positive relationship between the teeth examination-estimated and actual age in dairy cattle aged 45 months and below [r (20) = 0.97, p<0.001]. There was a very strong positive relationship between the teeth examination-estimated age and actual age in beef cattle aged over 45 months [r (35) = 0.93, p<0.001]. There was a very strong positive relationship between the teeth examination-estimated age and actual age in dairy cattle aged over 45 months [r (8) = 0.97, p<0.001]. It was therefore concluded that under the given conditions of the study and the range of ages and the breeds under study, age estimation through teeth examination is reliably applicable for aging cattle in general. Age estimation through teeth examination was found to be more accurate in dairy than in beef cattle.

African Swine Fever Outbreak at a Farm in Central Namibia.

An outbreak of African swine fever (ASF) occurred at a farm in central Namibia in March 2018. Fourteen pigs died out of a herd of 59 animals over a period of 16 days between the first and sixteenth of March 2018. The clinical signs observed included sternal recumbency, fever, weakness, pain and reluctance to move, hyperemia of the skin and anorexia, followed by death. Necropsy findings included large amounts of unclotted blood in the pleural and peritoneal cavities, diffuse carcass congestion, splenomegaly, consolidation of both lungs, hemorrhagic and frothy airways and trachea, hepatomegaly and congestion, congestion of the gastric mucosa, enlarged and congested kidneys, ecchymotic epicardial, and endocardial hemorrhages, and very enlarged and congested urinary bladder. All the remaining pigs were euthanized, burned, and buried under state veterinary supervision. The authors concluded that the outbreak resulted from indirect transmission of the ASF virus due to lapses in biosecurity measures.

Bovine rumen impaction caused by ingestion of Gonometa postica cocoons in eastern-central Namibia.

Cases of rumen impaction caused by ingestion of Gonometa postica cocoons occurred at a farm in eastern-central Namibia. Ten animals died on the farm over the previous 5 months. Rumenotomies were successfully performed on three affected animals. The authors were of the opinion that the affected animals ingested the cocoons around the time of weaning, which then resulted in tangled masses that gradually stretched and enlarged because of entrapment of ingesta, eventually causing impaction of the rumen in the young adult animals. These are the first reported cases of ruminal impaction attributable to G. postica cocoon ingestion in Namibia.

Characterization of Oestrus Cycles in Namibian Swakara and Damara Sheep through Determination of Circannual Plasma Progesterone Levels.

A year-long prospective study characterized the seasonality of oestrus cycles in primiparous, nonpregnant Swakara (n=8) and Damara (n=5) ewes through surveillance of plasma progesterone (P4) levels. During this period, Swakara and Damara groups evidently averaged 23 oestrus cycles with an average length of 17 days. Damara ewes showed greater mean peak plasma P4 levels (11.4±0.16ng/ml) than Swakara ewes (5.4±0.11ng/ml) (P<0.05). Oestrus cycles in Damara ewes showed relatively uniform plasma P4 peaks throughout the year ranging from 10.6±0.16 to 12.6±0.24ng/ml. In Swakara ewes, P4 peaks were highest in the autumn oestrus cycles (from 7.1±0.16 to 7.5±0.11ng/ml), rapidly declining through winter to 2.2±0.08ng/ml by midspring and then rapidly increasing to 4.9±0.37ng/ml at the commencement of summer, followed by a gradual increase from 5.7± to 7.1±ng/ml by the start of autumn. The annual mean area under the curve temporal progesterone measurements (AUCPM) in Damara ewes (115.9±18.6ng⁎day/ml) was greater than that in Swakara ewes (58.6±25.3ng⁎day/ml) (p<0.05). For Swakara ewes, the mean AUCPM in summer and autumn cycles (68.2±14.7 and 79.5±10.0ng⁎day/ml, respectively) were greater than those in spring and winter cycles (28.7±12.3 and 55.0±27.3ng⁎day/ml), respectively (P<0.05). There was no seasonal variation in the exposure of the Damara ewes to P4 in between seasons (P>0.05), though, however, the Damara ewes had greater P4 levels than the Swakara ewes (P<0.05). Progesterone profiles showed that Swakara ewes possessed 'residual' seasonality, whereas the Damara ewes were no longer seasonal. The implications of this disparity in the seasonal exposure of Swakara and Damara ewes to luteal P4 on fertility warrant further investigation.

Correction to: No evidence of Trichinella spp. in domestic pig carcasses at a selected abattoir in southern Botswana.

The article "No evidence of Trichinella spp. in domestic pig carcasses at a selected abattoir in southern Botswana", written by Basiamisi Ernest Segwagwe et al., was originally published electronically with open access. The authors decided to step back from Open Choice and trasnferred the copyright to the publisher.

Conception rates and calving intervals of different beef breeds at a farm in the semi-arid region of Namibia.

A retrospective study from 2004 to 2017 investigated the effect of bull age, cow age and breed on conception rates, and calving intervals of beef cattle at Neudamm farm, Khomas region, Namibia. Bulls ranging from 4 to 14 years of age were used to breed cows up to 17 years of age. A total of 1804 pregnancies were diagnosed in Afrikaner (81.8%), Nguni (14.1%), and Simmental (4.1%) cows. The overall conception rate of beef cattle during the study period was 71.7 ± 9.5%. The overall conception rate of the Nguni cows (78.3%) was significantly higher than that of the Afrikaner (70.9%) and the Simmental cows (64.9%) (p < 0.05). The age of sires had no effect on overall conception rate in all three breeds (p > 0.05). Afrikaner dams more than 10 years old had the least rate of conception (60.4%, p < 0.05) within that breed category. Nguni dams between four to 10 years had the greatest conception rate (86.5%, p < 0.05). Age of dams had no effect on conception rates in Simmental cattle (p > 0.05). The overall mean length of all calving intervals in the Nguni cows (366 ± 35 days) was significantly shorter than those of the Afrikaner cows (487 ± 62 days) and the Simmental cows (484 ± 110 days) (p < 0.05). The 2013 breeding season produced the highest overall conception rates (~ 85%), and the 2007 breeding season produced the lowest overall conception rate (~ 51%). From a fertility point of view, the Nguni breed seemed best suited for the semi-arid conditions which are prevalent in most of Namibia.

No evidence of Trichinella spp. in domestic pig carcasses at a selected abattoir in southern Botswana.

Trichinellosis is a worldwide zoonosis with genotypes affecting different domestic and wild animals and is widely distributed throughout the world. Species and genotypes of this genus affecting different animals have been identified. Despite its occurrence in Sub-Saharan countries, the presence of trichinellosis in Botswana is yet to be confirmed. The objective of this study was to determine the prevalence of Trichinella infection in domestic pigs slaughtered at an abattoir in Gaborone, Botswana. Of the 111 pig carcasses selected, 5 g of crus of the diaphragm was collected from each carcass, trimmed of all fat and fascia and then cut into 1- to 2-g samples. The muscle samples were pooled into 100-g muscle sample and then processed by the OIE prescribed digestion method. A stereomicroscope was used to examine each grid of the Petri dish for the presence of Trichinella larvae. No Trichinella larvae were found in any digested muscle samples. Future studies should target a wider pig population and other host animals.

Prevalence and risk factors for brucellosis seropositivity in cattle in Nyagatare District, Eastern Province, Rwanda.

A survey involving 120 small-scale dairy farmers was carried out to assess risk factors associated with brucellosis in cattle from selected sectors of Nyagatare District, Rwanda. A sample of cattle from nine selected sectors of Nyagatare was tested for brucellosis using the Rose Bengal Test. Of the respondents, 57.5% were unaware of brucellosis as a disease, 85.8% did not screen new additions to the herd for brucellosis and 82.5% did not remove brucellosis seropositive animals from the herd. The prevalence of brucellosis in herds with cows with no history of abortion was 38.5% and 17.0% in those with a history of abortion. None of the respondents disinfected abortion sites or vaccinated against brucellosis. The prevalence of brucellosis in cows with a history of retained placenta was 36% and 2% in those with no history of retained placenta. Of the respondents, 62.5% reportedly fed foetal membranes to dogs. About 65.8% of the respondents with brucellosis-positive animals reported a calving interval longer than 1 year. Katabagemu (28.6%) had the highest prevalence of brucellosis seropositivity while Karama had none. Brucellosis in cows (21.4%) was significantly higher than that in heifers (12.8%) (p < 0.05), but there was no significant difference between heifers and bulls or between bulls and cows (p > 0.05). The occurrence of brucellosis in herds with 40-70 cattle (26.9%) was significantly greater than the 14.9% of herds with 10-39 cattle (p < 0.05). Seropositivity to brucellosis in cross-breed cattle (23.6%) was significantly greater than that in indigenous cattle (13.8%) (p < 0.05). There was no significant difference in the overall prevalence of brucellosis in cattle from different grazing systems (p > 0.05). Seropositivity to brucellosis was significantly different (p < 0.05) between the fourth parity (32.5%) and first parity (14.3%) cows. The findings in this study confirmed the existence of brucellosis as a problem in Nyagatare and the authors recommend that farmer education on the epidemiology, risk factors and mitigation of the disease be undertaken as a matter of urgency.

Quantitative Characterization of the Hemorrhagic, Necrotic, Coagulation-Altering Properties and Edema-Forming Effects of Zebra Snake (Naja nigricincta nigricincta) Venom.

This study was designed to investigate the cytotoxicity and haemotoxicity of the Western barred (zebra) spitting cobra (Naja nigricincta nigricincta) venom to help explain atypical and inconsistent reports on syndromes by Namibian physicians treating victims of human ophidian accidents. Freeze-dried venom milked from adult zebra snakes was dissolved in phosphate buffered saline (PBS) for use in this study. Haemorrhagic and necrotic activity of venom were studied in New Zealand albino rabbits. Oedema-forming activity was investigated in 10-day-old Cobb500 broiler chicks. Procoagulant and thrombolytic activity was investigated in adult Kalahari red goat blood in vitro. The rabbit skin minimum hemorrhagic dose (MHD) for N. n. nigricincta was 9.8µg. The minimum necrotizing dose (MND) for N. n. nigricincta venom was 12.2µg. The N. n. nigricincta venom showed linear dose-dependent procoagulant activity on goat blood (p<0.05). Likewise, N. n. nigricincta venom showed linear dose-dependent thrombolytic activity on goat blood (p<0.05, n = 6). Subplantar injection of N. n. nigricincta venom (25µg, 50µg, 75µg, and 100µg) into chick paw resulted in peak oedema of 35.5%, 38.5%, 42.9%, and 47.5%, respectively, two hours after injection. Paw oedema subsided within five hours to a mean volume ranging from 5% (25µg venom) to 17.6% (100µg venom). In conclusion, though N. n. nigricincta belongs to the genus Elapidae, the current study has shown its venom to possess potent hemorrhagic, necrotic (cytotoxic), and paradoxically, both procoagulant and thrombolytic activity. The authors propose further work to fractionate, isolate, and elucidate the structure of the various N. n. nigricincta venom toxins as a prelude to the development of an antivenom.

Prevalence of Haemonchus contortus Infections in Sheep and Goats in Nyagatare District, Rwanda.

This study investigated the overall prevalence of Haemonchus contortus infection in sheep and goats from five purposively selected subdivisions (sectors) of Nyagatare district from January to December 2014, after a high prevalence of gastrointestinal parasites and generalized poor productivity was reported in small ruminants in some districts of Rwanda. Faecal egg counts (FEC) were performed using the Modified Wisconsin Sugar Floatation method and the Fluorescent-labeled peanut-lectin agglutination test while enumerations, as log (FEC), were done using the modified McMaster method. The overall prevalence of H. contortus infection in sheep and goats was 75.7% (n=949). The overall prevalence of H. contortus infection in sheep (83.4%, n=314) was higher than in goats (71.8%, n=635) (Odds Ratio [OR] 1.98, 95% Confidence Interval [CI]: 1.40-2.79, and p≤0.001). The prevalence of H. contortus infection in female goats (74.2%) was higher than in male goats (64.3%) (OR 1.60, 95% CI: 1.09-2.36, and p=0.01). The prevalence of H. contortus infection in goats from Nyagatare was higher than in goats from Matimba (OR 3.25, 95% CI: 1.76-5.99, and p≤0.001) and from Katabagemu (OR 3.67, 95% CI: 2.04-6.59, and p≤0.001). The prevalence of H. contortus infection in goats from Karangazi was higher than in goats from Matimba (OR 4.72, 95% CI: 2.40-9.28, and p≤0.001). The overall mean monthly log (FEC) for H. contortus in sheep and goats were highest in April (18.9±0.2 and 14.05±0.1, respectively) and October (19.25± 0.2 and 13.75±0.1, respectively). Though, overall, sheep in Nyagatare district were at greater risk of H. contortus infection and goats from Nyagatare and Karangazi sectors were paradoxically at greater risk of H. contortus infection. It was also apparent that young female goats were at greater risk of H. contortus infection than young male goats. H. contortus infection is endemic in small ruminants in Nyagatare district and possibly other districts in Rwanda. Targeted selective treatment (TST) using FAMACHA with emphasis on low-lying swampy pastures and appropriate anthelmintic drugs may be the most economically viable solution in the short term. In the long term breeding of H. contortus resistant small ruminants and strategic grazing using the concept of refugia may bring about considerable relief from H. contortus infection in Nyagatare district, in particular, and Rwanda at large.

An epidemiological survey of the magnitude and local perceptions of porcine cysticercosis by two methods in Nyaruguru district, Rwanda.

This study investigated the magnitude of porcine cysticercosis (PC), its risk factors, economic effects and the perceptions of 80 pig farmers from Nyabimata (n = 38) and Muganza (n = 42) and 20 registered butchers in the Nyaruguru district of Rwanda. January to December 2013 slaughter records from Kamirabagenzi market were also analysed for PC diagnoses based on the tongue test and meat inspection. During this period, the responding farmers' records showed a tongue test-based PC magnitude (3.9%, n = 984) which was lower than the collective tongue test-based PC magnitude of 9.2% (n = 1720) at Kamirabagenzi (p < .05). The overall magnitude of PC based on routine meat inspection diagnosis at Kamirabagenzi was 4%. The overall magnitude of PC for respondents using Free-range production systems (7.9%) was significantly greater than for those in Semi-intensive (2.1%) and Intensive production systems (1.5%) (p < .05). Though most farmers (90%) knew that PC is zoonotic, only 22.5% of the farmers opted for treatment of PC-infected pigs and 52.5% were willing to seek veterinary inspection while the rest (25%) opted to circumvent veterinary inspection (P > .05). Most butchers (70%) indicated they would circumvent veterinary inspection and continue to slaughter PC-positive animals whilst the rest (30%) indicated they would resell PC positive animals to defray costs (P < .05). The low sensitivity and specificity of methods used for PC detection in the study, implies that this may just be the tip of an iceberg and the actual magnitude is most likely to be much higher. In conclusion, PC is endemic in the Nyaruguru district of Rwanda with a high proportion of positive animals. The condition has public health implications and is worsening the economic plight of the impoverished Nyaruguru community.

A 4-year study of the proportional distribution of male reproductive organ abnormalities in cattle slaughtered at Nyagatare abattoir, Eastern Rwanda.

Male reproductive performance has more impact on overall herd productivity than in the female. In order to assess herd productivity in cattle in Nyagatare, Eastern Rwanda, the proportional distribution of male reproductive organ abnormalities was investigated in 3087 bulls slaughtered over a 4-year period. The aim of the study was to investigate the proportional distribution of male reproductive organ abnormalities in exotic and indigenous bulls slaughtered over a 4-year period at Nyagatare abattoir in Eastern Rwanda. Positioning of the testicles was observed and recorded as the animals were assembled before slaughter. After slaughter, the internal and external reproductive organs of all bulls were removed, labelled and analysed for pathology. Significantly more indigenous (79.01%) than exotic (20.99%) animals were slaughtered (P < 0.05; N = 3 087). Overall occurrence of abnormalities was significantly higher in exotic (20.83%) than in indigenous (10.33%) animals (P < 0.05). Overall, abnormal location of testicles was the most common abnormality (4.08%) followed by abnormal prepuce and penis (2.33%), orchitis (1.94%), enlarged vesicular gland (0.91%), scrotal hernia (0.87%), unilateral cryptorchidism (0.81%), testicular hypoplasia (0.81%) and hydrocoele (0.78%). Abnormal location of testicles, abnormal prepuce and penis, orchitis, testicular hypoplasia and enlarged vesicular glands had significantly higher prevalence in exotic animals than in indigenous animals (P < 0.05). There was no significant difference in the prevalence of unilateral cryptorchidism, hydrocoele and scrotal hernia between the exotic and indigenous breeds. The encountered reproductive abnormalities result in poor herd fertility manifesting as low first-service conception rates, prolonged breeding seasons and reduced weaning weights accompanied by inevitable financial losses in beef production. The observed high proportion of male reproductive abnormalities in exotic breeds might offset the professed benefits from introduction of these exotic breeds with the aim of improving productivity.

A 3-year prospective study of the incidence of gastric ulcers in pigs slaughtered at Base Abattoir in Rwanda.

AIM: Determination of the incidence of gastric ulcers in pigs slaughtered at Base Abattoir in Rwanda. MATERIALS AND METHODS: Stomachs from all 5040 pigs that were slaughtered at Base Abattoir in Rwanda from August 2012 to August 2015 were examined for the presence and location of gastric ulcers. The results of the inspections were recorded and analyzed. Statistical analysis for Chi-square values was performed using the Software Package for Social Sciences version 16.0. The Z test for comparison of proportions was used and p<0.05 was considered significant. RESULTS: Overall as well as per district, significantly more male pigs than female pigs were slaughtered (p<0.05). The incidence of gastric ulcers in slaughter pigs was 12.86%. The incidence of gastric ulcers in males (13.36%) was not significantly different (p>0.05) from that in female pigs (12.84%) in all three districts. A significantly greater proportion of these ulcers (69.03%) was located in the esophageal region than in the glandular region of the stomach (30.97%) in slaughtered males (p<0.05). A significantly greater proportion of these ulcers (79.59%) was located in the esophageal region than in the glandular region of the stomach (20.41%) in slaughtered females (p<0.05). The overall incidence of esophageal ulcers (9.44%) in the slaughtered pigs was significantly (p<0.05) higher than that of glandular region ulcers (3.41%). Pigs with carcass weight over 60 kg showed a significantly (p<0.05) higher (44.44%) incidence of ulcers than those between 40 and 60 kg (33.33%) and those below 40 kg (22.22%). CONCLUSIONS: It was concluded that the incidence of gastric ulceration in slaughter pigs at Base Abattoir was not associated with source (district) or sex of pigs but was associated with the carcass weight.

Comparison of cow-side diagnostic tests for subclinical mastitis of dairy cows in Musanze district, Rwanda.

Four subclinical mastitis diagnostic tests (the UdderCheck® test [a lactate dehydrogenasebased test], the California Mastitis Test [CMT], the Draminski® test [a conductivity-based test] and the PortaSCC® test [a portable somatic cell count-based test]) were compared in a study comprising crossbreed dairy cows (n = 30) during September and October 2015. Sensitivity and specificity of the CMT, Draminski® and UdderCheck® tests were compared with the PortaSCC® as reference. The CMT, Draminski® and UdderCheck® test results were compared with the results of the PortaSCC® test using kappa statistics. Duplicate quarter milk samples (n = 120) were concurrently subjected to the four tests. Sensitivity and specificity were 88.46% and 86.17% (CMT), 78.5% and 81.4% (Draminski®) and 64.00% and 78.95% (UdderCheck®). The CMT showed substantial agreement (k = 0.66), the Draminski® test showed moderate agreement (k = 0.48) and the UdderCheck® test showed fair agreement (k = 0.37) with the PortaSCC® test and positive likelihood ratios were 6.40, 4.15 and 3.04, respectively. The cow-level subclinical mastitis prevalence was 70%, 60%, 60% and 56.7% for PortaSCC®, CMT, Draminski® and UdderCheck® tests, respectively. At udder quarter level, subclinical mastitis prevalence was 20%, 21.67% and 20.83% for PortaSCC®, CMT and UdderCheck®, respectively. A correlation (P < 0.05) and moderate strength of association were found between the four tests used. The study showed that compared to the PortaSCC® test, the CMT was the most preferable option, followed by the Draminski® test, while the UdderCheck® test was the least preferable option for subclinical mastitis screening.