RESUMO
Organic insecticides are well known neurotoxicants. Nitric oxide (NO) is a neurotransmitter formed stoicheometrically with citrulline from L-arqinine through mediation of the enzyme nitric oxide synthase (NOS). We measured NOS activity in rat brain in vitro in the presence of selected organic insecticides such as, 10-200 microM conc carbaryl, kepone and malathion. All these three compounds inhibited NOS activity of rat brain in vitro in a concentration dependent manner. In most cases the changes observed were statistically significant. The order of potency, based on IC50 values of these insecticides, to inhibit NOS activity of rat brain, is carbaryl (105 microM) > Kepone (144 microM) > malathion (170 microM). We further demonstrated that these insecticides inhibit calmodulin (CaM)-stimulated NOS activity without affecting the basal enzyme activity. It is reported that the observed inhibition of NOS by selected insecticides may be due to interaction of these insecticides with Ca2+/CaM on which the NOS activity is well known to be dependent. This ultimately may lead to neurotoxicity of rat brain.
Assuntos
Encéfalo/efeitos dos fármacos , Encéfalo/enzimologia , Carbaril/toxicidade , Clordecona/toxicidade , Malation/toxicidade , Óxido Nítrico Sintase/metabolismo , Animais , Cálcio/metabolismo , Calmodulina/metabolismo , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/toxicidade , Inseticidas/toxicidade , Masculino , Óxido Nítrico Sintase/antagonistas & inibidores , Ratos , Ratos Sprague-DawleyAssuntos
Ácido Araquidônico/urina , Ciclosporina/antagonistas & inibidores , Ciclosporina/toxicidade , Imunossupressores/antagonistas & inibidores , Imunossupressores/toxicidade , Rim/efeitos dos fármacos , Vitamina E/farmacologia , Animais , Ácido Araquidônico/metabolismo , Creatinina/metabolismo , Dinoprosta/urina , Radicais Livres/metabolismo , Rim/metabolismo , Masculino , Nefrectomia , Prostaglandinas F/urina , Ratos , Ratos Sprague-Dawley , Tromboxano B2/urinaRESUMO
BACKGROUND: Indomethacin causes gastric mucosal injury, although the pathogenesis is not fully understood. Zinc, is known to have gastroprotective effects in both humans and experimental animals. AIM: To determine (i) the protective effects of zinc in indomethacin-induced gastric mucosal injury in rats, and (ii) whether these cytoprotective effects are mediated by changes in gastric lipid peroxidation and/or nitric oxide synthase activity. METHODS: Gastric lesions were induced in rats by the intragastric administration of indomethacin. Morphological changes, lipid peroxidation (malondialdehyde levels) and nitric oxide synthase activity were determined in animals pre-treated with zinc sulphate and in controls. RESULTS: Indomethacin significantly increased malondialdehyde levels and decreased NOS activity. These effects were attenuated by pre-treatment with zinc (P < 0.005 and 0.0001, respectively). The protective effects of zinc were readily abolished in animals pre-treated with N-nitro-L-arginine methyl ester (L-NAME). Morphologically, indomethacin induced large areas of mucosal ulcerations, which were completely prevented by zinc pre-treatment. CONCLUSIONS: Zinc provides protection against indomethacin-induced gastric mucosal injury. These protective effects result from the inhibition of lipid peroxidation and the preservation of mucosal nitric oxide synthase.
Assuntos
Anti-Inflamatórios não Esteroides/toxicidade , Mucosa Gástrica/efeitos dos fármacos , Indometacina/toxicidade , Peroxidação de Lipídeos/efeitos dos fármacos , Óxido Nítrico/fisiologia , Zinco/farmacologia , Animais , Mucosa Gástrica/patologia , Masculino , Óxido Nítrico Sintase/metabolismo , Óxido Nítrico Sintase Tipo II , Ratos , Ratos Sprague-DawleyRESUMO
Acephate (AT) is an organophosphate (OP) insecticide. Due to their reputation for low environmental persistence, OP pesticides are often used indiscriminately resulting in detrimental exposure to humans and other nontarget species. Although the toxicity of OP compounds is primarily through blockade of neural transmission via inhibition of acetylcholinesterase, studies have revealed histopathological alterations in the renal proximal tubules, suggesting a role for additional mechanisms in renal toxicity. It is our hypothesis that Reactive Oxygen Species (ROS) may play a role in OP-induced renal tubular injury for the following reasoning. Renal tubular cells concentrate many nephrotoxic chemicals including OPs, and renal injury from many of these compounds has been shown to arise from excessive ROS production. Furthermore, it has been established that many phosphorothiolates, which are sulfur-containing OPs and constitute the class of OP compounds to which AT belongs, are S-oxidized to highly reactive intermediates within cells and tissues. Because of these considerations, we examined whether ROS play a role in OP-induced renal tubular epithelial cell (LLC-PK1) toxicity using AT as a prototype. AT produced a concentration- and time-dependent increase in cell damage in LLC-PK1 cells, measured by lactate dehydrogenase (LDH, % of total) leakage. The cytotoxicity (LDH) induced by 2500 ppm of AT over 72 h was significantly suppressed by antioxidants 2-methylaminochroman (2-MAC) and desferrioxamine (DFO). H2O2 levels were significantly elevated following exposure of LLC-PK1 cells to 2500 ppm of AT. Malondialdehyde (MDA) formation was also significantly increased in AT-exposed cells compared to the control cells, indicating the occurrence of enhanced lipid peroxidation. 2-MAC and DFO, in addition to providing cytoprotection, inhibited AT-induced MDA generation in a significant and concentration-dependent manner. Results from this study, which is the first to explore the toxic effects of AT on renal tubular cells, demonstrate that toxic action of AT on kidney cells is partly through an ROS-mediated mechanism. Based on these direct in vitro findings, we further hypothesize that oxidant stress may play a role in the pathogenesis of AT-induced acute tubular necrosis and renal dysfunction observed in cases of AT overdoses.
Assuntos
Inseticidas/toxicidade , Túbulos Renais/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Compostos Organotiofosforados/toxicidade , Estresse Oxidativo/fisiologia , Espécies Reativas de Oxigênio/fisiologia , Animais , Antioxidantes/farmacologia , Células Cultivadas , Cromanos/farmacologia , Desferroxamina/farmacologia , Relação Dose-Resposta a Droga , L-Lactato Desidrogenase/análise , Fosforamidas , Piperazinas/farmacologia , Suínos , Fatores de TempoRESUMO
Fructose-1,6-diphosphate (FDP) was found to cause significant stimulation of nitric oxide synthase (NOS) in rat liver homogenates in vitro. This effect was more pronounced for the inducible isoform than its constitutive counterpart. Furthermore, FDP restored rat liver inducible NOS levels following their depletion by carbon tetrachloride (CCl4). This finding may have further practical implications in hepatoprotection from various noxious chemical and biological agents.
Assuntos
Frutosedifosfatos/farmacologia , Fígado/efeitos dos fármacos , Óxido Nítrico Sintase/biossíntese , Animais , Tetracloreto de Carbono/farmacologia , Indução Enzimática , Fígado/enzimologia , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
Cyclosporin-A, a potent immunosuppressive agent is known to induce cellular toxic side effects by way of altering calcium homeostasis, including calcium/calmodulin mediated events. We studied the in vitro and in vivo effects of cyclosporin-A on rat brain nitric oxide synthase activity (the enzyme that mediates the conversion of L-arginine to citrulline and NO). CsA in concentrations of 22-4400 nM inhibited rat brain NOS activity in vitro and in 10 or 25 mg/kg wt/4 weeks of CsA treated rat brains in vivo. We report here that CsA by way of interfering with rat brain Ca2+/CaM mediated events may inhibit its NOS activity which ultimately may result in neurotoxicity.
Assuntos
Encéfalo/enzimologia , Ciclosporina/farmacologia , Inibidores Enzimáticos/farmacologia , Imunossupressores/farmacologia , Óxido Nítrico Sintase/antagonistas & inibidores , Animais , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
Cyclosporin A (CsA) is a cyclic undecapeptide that has been used extensively as an immunosuppressive drug in transplantation medicine and is known to interact with L-arginine dependent pathways. We studied the in vitro and in vivo effects of CsA on nitric oxide synthase (NOS) activity in the rat kidney. CsA in concentrations of 44-2200 nM in vitro, and 12.5 or 25 mg/kg body weight per 4 weeks treated rat kidneys in vivo, significantly stimulated NOS activity. CsA may alter the Ca2+/Cam-dependent NOS activity by interacting with rat kidney Ca2+/calmodulin dependent events.
Assuntos
Ciclosporina/farmacologia , Imunossupressores/farmacologia , Rim/enzimologia , Óxido Nítrico Sintase/metabolismo , Animais , Técnicas In Vitro , Rim/efeitos dos fármacos , Nefropatias/induzido quimicamente , Nefropatias/metabolismo , Masculino , Proteínas/metabolismo , Ratos , Ratos Sprague-DawleyRESUMO
OBJECTIVE: The importance of portal hypertensive gastropathy, as a potentially bleeding lesion in cirrhotics with portal hypertension, has recently been appreciated. Histologically, dilation of the mucosal and submucosal vessels of the stomach is noted in this entity. The possibility of nitric oxide acting as a mediator for this mucosal vascular dilation has not been explored. METHODS: We determined, in a group of 10 male cirrhotic patients with esophageal varices and endoscopic changes consistent with severe portal hypertensive gastropathy (Group A), the gastric mucosal nitric oxide synthase activity. This was determined by measuring the rate of conversion of [3H]-arginine to [3H]-citrulline. Serum levels of nitrates and nitrites, the end products of nitric oxide, were also measured. The results were compared with those of a group of 10 male controls with no liver disease (Group B). RESULTS: Gastric mucosal constitutive and inducible nitric oxide synthase levels were significantly higher in group A (125.4 +/- 4.3 and 259.7 +/- 5.5 pmol/mg protein/minute, respectively) than in group B (88 +/- 8.6 and 130.8 +/- 6.6 pmol/mg protein/minute, respectively) ( p < 0.002 and < 0.0001, respectively). Serum nitrate/nitrite levels were 30.1 +/- 3.2 nmol/ml in group A and 15.5 +/- 0.09 nmol/ml in group B (p < 0.001). CONCLUSIONS: We conclude that the significantly increased gastric mucosal nitric oxide synthase activity, in patients with portal hypertensive gastropathy, suggests an important role for nitric oxide in the pathogenesis of this mucosal lesion.
Assuntos
Varizes Esofágicas e Gástricas/enzimologia , Mucosa Gástrica/enzimologia , Hipertensão Portal/enzimologia , Óxido Nítrico Sintase/metabolismo , Adulto , Idoso , Varizes Esofágicas e Gástricas/patologia , Mucosa Gástrica/patologia , Humanos , Hipertensão Portal/patologia , Cirrose Hepática/enzimologia , Cirrose Hepática/patologia , Masculino , Pessoa de Meia-Idade , Nitratos/sangue , Nitritos/sangue , Estudos Prospectivos , Antro Pilórico/patologiaRESUMO
21-Aminosteroids have incited a great deal of interest owing to its ability to inhibit lipid peroxidation and prevent organ damage. The main mechanism by which 21-aminosteroids inhibit lipid peroxidation is similar to the naturally occurring chain-breaking antioxidant alpha-tocopherols. Therefore, to determine whether 21-aminosteroids offer any advantage over alpha-tocopherol, we compared their effects on an in vivo and in vitro models of renal injury. 21-Aminosteroid (U-74006 F) at 3 mg/kg or alpha-tocopherol succinate at 10 mg/kg was administered intravenously once before bilateral renal ischemia and again before reperfusion. Acute administration 21-aminosteroid but not alpha-tocopherol, was attended by suppression of ischemia reperfusion-induced renal lipid peroxidation and injury. However, 4 weeks of dietary enrichment of rats with alpha-tocopherol (1000 IU/kg) was effective in suppressing these ischemia reperfusion-induced changes. In cell culture system, concurrent presence of 21-aminosteroid but not alpha-tocopherol abrogated H2O2-induced renal epithelial lipid peroxidation and injury. However, alpha-tocopherol was completely effective when cells were incubated with it for 14 h. Further, only the cells incubated with vitamin E for 14 h-but not for 1 or 3 h-had a significant increase in vitamin E content, which suggests that a delay in prompt cellular up take of vitamin E may explain its lack of acute effects. Thus, unlike alpha-tocopherol, 21-aminosteroid appears readily and completely available for its chain-breaking antioxidant activity both in vitro and in vivo. 21-Aminosteroids may, therefore, offer a therapeutic advantage over alpha-tocopherols in acute injury settings.