RESUMO
In this study, a method for determination of cholesterol and four phytosterols by gas chromatography coupled with electron impact ionization mode-tandem mass spectrometry without derivatization in general food was developed. The sample was saponified with 7.5% KOH in methanol. After heating on hot plate and reflux for 60 minutes, the saponified portion was extracted with n-hexane/petroleum ether (50:50, v/v). The extracts were evaporated with rotary evaporator and then redissolved with tetrahydrofuran. The tetrahydrofuran layer was transferred into an injection vial and analyzed by gas chromatography on a 30 m VF-5 column. Limit of quantification was 2 mg/kg. Recoveries of cholesterol and four phytosterols from general food were between 91% and 100%.
RESUMO
The structures, names, bioactivities and references of 105 natural products obtained from gorgonian corals belonging to the family Plexauridae with an Indo-Pacific distribution are described in this review. All compounds mentioned in this review were obtained from gorgonian corals belonging to the genera Astrogorgia, Bebryce, Echinomuricea, Euplexaura and Menella.
Assuntos
Antozoários/química , Produtos Biológicos/isolamento & purificação , Animais , Produtos Biológicos/química , Produtos Biológicos/farmacologia , Oceano Índico , Oceano PacíficoRESUMO
A new germacrane-type sesquiterpenoid, menelloide E (1), and a new cembrane-type diterpenoid, lobocrassin F (2), were isolated from the octocorals Menella sp. and Lobophytum crassum, respectively. The structures of terpenoids 1 and 2 were determined by spectroscopic and chemical methods and compound 2 was found to display a significant inhibitory effect on the release of elastase by human neutrophils.
Assuntos
Antozoários/metabolismo , Anti-Inflamatórios não Esteroides/isolamento & purificação , Anti-Inflamatórios não Esteroides/farmacologia , Terpenos/isolamento & purificação , Terpenos/farmacologia , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/metabolismo , Aquicultura , Misturas Complexas/química , Misturas Complexas/farmacologia , Diterpenos/química , Diterpenos/isolamento & purificação , Diterpenos/metabolismo , Diterpenos/farmacologia , Descoberta de Drogas , Espécies em Perigo de Extinção , Humanos , Elastase de Leucócito/metabolismo , Estrutura Molecular , Ativação de Neutrófilo/efeitos dos fármacos , Neutrófilos/efeitos dos fármacos , Neutrófilos/imunologia , Neutrófilos/metabolismo , Oceano Pacífico , Sesquiterpenos/química , Sesquiterpenos/isolamento & purificação , Sesquiterpenos/metabolismo , Sesquiterpenos/farmacologia , Estereoisomerismo , Taiwan , Terpenos/química , Terpenos/metabolismoRESUMO
Two new metabolites, including a lindenane-type sesquiterpenoid, menelloide C (1), and a germacrane-type sesquiterpenoid, menelloide D (2), were isolated from a Formosan gorgonian coral identified as Menella sp. The structures of 1 and 2 were established by spectroscopic methods and 2 displayed a weak inhibitory effect on the release of elastase by human neutrophils.
Assuntos
Antozoários/metabolismo , Sesquiterpenos/isolamento & purificação , Animais , Humanos , Espectroscopia de Ressonância Magnética , Elastase Pancreática/antagonistas & inibidores , Sesquiterpenos/química , Sesquiterpenos/farmacologiaRESUMO
(-)-Hydroxylindestrenolide (1), an enantiomer of the known sesquiterpenoid, (+)-hydroxylindestrenolide (2), was isolated from a gorgonian coral identified as Menella sp. The structure, including the relative stereochemistry of 1 was established by spectroscopic methods and by comparison the spectral and physical data with those of 2. Sesquiterpenoid 1 exhibited marginal cytotoxicity toward HL-60 and K562 leukemia tumor cells, and displayed a weak inhibitory effect on superoxide anion generation at a concentration of 10 µg/ml by human neutrophils.
