Microbiological hydrogen (H2 ) thresholds in anaerobic continuous-flow systems: Effects of system characteristics.

Hydrogen (H2 ) concentrations that were associated with microbiological respiratory processes (RPs) such as sulfate reduction and methanogenesis were quantified in continuous-flow systems (CFSs) (e.g., bioreactors, sediments). Gibbs free energy yield (ΔÇ´ ~ 0) of the relevant RP has been proposed to control the observed H2 concentrations, but most of the reported values do not align with the proposed energetic trends. Alternatively, we postulate that system characteristics of each experimental design influence all system components including H2 concentrations. To analyze this proposal, a Monod-based mathematical model was developed and used to design a gas-liquid bioreactor for hydrogenotrophic methanogenesis with Methanobacterium bryantii M.o.H. Gas-to-liquid H2 mass transfer, microbiological H2 consumption, biomass growth, methane formation, and Gibbs free energy yields were evaluated systematically. Combining model predictions and experimental results revealed that an initially large biomass concentration created transients during which biomass consumed [H2 ]L rapidly to the thermodynamic H2 -threshold (≤1 nM) that triggerred the microorganisms to stop H2 oxidation. With no H2 oxidation, continuous gas-to-liquid H2 transfer increased [H2 ]L to a level that signaled the methanogens to resume H2 oxidation. Thus, an oscillatory H2 -concentration profile developed between the thermodynamic H2 -threshold (≤1 nM) and a low [H2 ]L (~10 nM) that relied on the rate of gas-to-liquid H2 -transfer. The transient [H2 ]L values were too low to support biomass synthesis that could balance biomass losses through endogenous oxidation and advection; thus, biomass declined continuously and disappeared. A stable [H2 ]L (1807 nM) emerged as a result of abiotic H2 -balance between gas-to-liquid H2 transfer and H2 removal via advection of liquid-phase.

Experimental data for physical characteristics, fiber compositions, and tensile properties of nonwoven wipes and toilet papers.

This article presents experimental data for physical characteristics, fiber compositions, and tensile properties of non-flushable wipes, flushable wipes, and toilet papers. Samples included 42 flushable wipes, 16 non-flushable wipes, and 11 toilet papers that were collected from around the world by considering product diversity in their retail regions (e.g., north america, and europe), manufacturers (e.g., global, and regional), and function (e.g., baby, toddler, patient, adult, and feminine wipes). The data were generated in accordance with relevant standard methods of International Organization for Standardization (ISO). The data are provided here in full (not hosted by any public repository) in association with the research article: "Physical characteristics, fiber compositions, and tensile properties of nonwoven wipes and toilet papers in relevance to what is flushable" [1]. Readers are referred to the research article for discussions and interpretations of the data presented in this document.

Physical characteristics, fiber compositions, and tensile properties of nonwoven wipes and toilet papers in relevance to what is flushable.

Numerous products, such as moist wipes, are marketed worldwide as "flushable." Recent studies indicate that wipes cause operational problems (e.g., pipe blockages) in sewer systems. This study investigates potential reasons for why wipes are problematic in sewer operations. Physical characteristics, fiber compositions, and tensile properties of non-flushable wipes, flushable wipes, and toilet papers (TPs) were assessed. Flushables, non-flushables, and TPs, respectively, had sheet masses of 1.5, 1.5, 0.5 g; surface areas of 250, 300, and 120 cm2 per sheet; thicknesses of 360, 370, and 160 µm; and volumes of 9.2, 11, 1.9 cm3 per sheet. While TPs were made of only plant-based fibers, wipes contained plant-based, and regenerated-cellulose (RC) fibers at various ratios, including up to 100% RC fibers. For tensile strength, the maximum force to break a specimen (Fmax) averaged 3 N for dry TPs, and 0.26 N for wet TPs. In contrast, the average Fmax values were 7 N for dry flushables and 5.9 N for their wet sheets. In wet states, TPs lost their strength by an average of 91%, but flushable wipes had variable changes: some wipes gained wet strength by 25%, some lost as much as 90%, and the average effect was a reduction by 29%. Thus, nonwoven wipes retain their strength and structure when wet, presumably because they contain RC fibers, which are known for their high wet strength. Accordingly, using synthetic fibers in flushable wipes seems to be the key reason for why the wipes cause operational problems in sewer systems.

Role of hydrogen (H2) mass transfer in microbiological H2-threshold studies.

Gas-to-liquid mass transfer of hydrogen (H2) was investigated in a gas-liquid reactor with a continuous gas phase, a batch liquid phase, and liquid mixing regimes relevant to assessing kinetics of microbial H2 consumption. H2 transfer was quantified in real-time with a H2 microsensor for no mixing, moderate mixing [100 rotations per minute (rpm)], and rapid mixing (200 rpm). The experimental results were simulated by mathematical models to find best-fit values of volumetric mass transfer coefficients-kLa-for H2, which were 1.6/day for no mixing, 7/day for 100 rpm, and 30/day for 200 rpm. Microbiological H2-consumption experiments were conducted with Methanobacterium bryantii M.o.H. to assess effects of H2 mass transfer on microbiological H2-threshold studies. The results illustrate that slow mixing reduced the gas-to-liquid H2 transfer rate, which fell behind the rate of microbiological H2 consumption in the liquid phase. As a result, the liquid-phase H2 concentration remained much lower than the liquid-phase H2 concentration that would be in equilibrium with the gas-phase H2 concentration. Direct measurements of the liquid-phase H2 concentration by an in situ probe demonstrated the problems associated with slow H2 transfer in past H2 threshold studies. The findings indicate that some of the previously reported H2-thresholds most likely were over-estimates due to slow gas-to-liquid H2 transfer. Essential requirements to conduct microbiological H2 threshold experiments are to have vigorous mixing, large gas-to-liquid volume, large interfacial area, and low initial biomass concentration.

Effect of turbulence on the disintegration rate of flushable consumer products.

A previously developed model for the physical disintegration of flushable consumer products is expanded by investigating the effects of turbulence on the rate of physical disintegration. Disintegration experiments were conducted with cardboard tampon applicators at 100, 150, and 200 rotations per minute, corresponding to Reynold's numbers of 25,900, 39,400, and 52,900, respectively, which were estimated by using computational fluid dynamics modeling. The experiments were simulated with the disintegration model to obtain best-fit values of the kinetic and distribution parameters. Computed rate coefficients (ki) for all solid sizes (i.e., greater than 8, 4 to 8, 2 to 4, and 1 to 2 mm) increased strongly with Reynold's number or rotational speed. Thus, turbulence strongly affected the disintegration rate of flushable products, and the relationship of the ki values to Reynold's number can be included in mathematical representations of physical disintegration.

Physical disintegration of toilet papers in wastewater systems: experimental analysis and mathematical modeling.

Physical disintegration of representative toilet papers was investigated in this study to assess their disintegration potential in sewer systems. Characterization of toilet papers from different parts of the world indicated two main categories as premium and average quality. Physical disintegration experiments were conducted with representative products from each category according to standard protocols with improvements. The experimental results were simulated by mathematical model to estimate best-fit values of disintegration rate coefficients and fractional distribution ratios. Our results from mathematical modeling and experimental work show that premium products release more amounts of small fibers and disintegrate more slowly than average ones. Comparison of the toilet papers with the tampon applicators studied previously indicates that premium quality toilet papers present significant potential to persist in sewer pipes. Comparison of turbulence level in our experimental setup with those of partial flow conditions in sewer pipes indicates that drains and small sewer pipes are critical sections where disintegration of toilet papers will be limited. For improvement, requirements for minimum pipe slopes may be increased to sustain transport and disintegration of flushable products in small pipes. In parallel, toilet papers can be improved to disintegrate rapidly in sewer systems, while they meet consumer expectations.

Development of a mathematical model for physical disintegration of flushable consumer products in wastewater systems.

The processes that flushable solid products may undergo after discharge to wastewater systems are (1) physical disintegration of solids resulting from turbulence, (2) direct dissolution of water-soluble components, (3) hydrolysis of solids to form soluble components, and (4) biodegradation of soluble and insoluble components. We develop a mathematical model for physical disintegration of flushable solid consumer products and test it with two different flushable products--product A, which has 40% water soluble-content, and product B, which has no water-soluble components. We present our modeling analysis of experimental results, from which we computed disintegration rate constants and fractional distribution coefficients for the disintegration of larger solids. The rate constants for solids of product A in units of (hour(-1)) are 0.45 for > 8-mm, 2.25 x 10(-2) for 4- to 8-mm, 0.9 x 10(-2) for 2- to 4-mm, and 1.26 x 10(-2) for 1- to 2-mm solids. The rate constants for solids of product B in units of hour(-1) are 1.8 for > 8-mm, 1.8 for 4- to 8-mm, 3.6 x 10(-1) for 2- to 4-mm, and 2.25 x 10(-3) for 1- to 2-mm solids. As indicated by the rate constants, larger solids disintegrate at a faster rate than smaller solids. In addition, product B disintegrated much faster and went mostly to the smallest size range, while product A disintegrated more slowly and was transferred to a range of intermediate solid sizes.

A mathematical model for the kinetics of Methanobacterium bryantii M.o.H. considering hydrogen thresholds.

We develop a kinetic model that builds on the foundation of classic Monod kinetics, but incorporates new phenomena such as substrate thresholds and survival mode observed in experiments with the H2-oxidizing methanogen Methanobacterium bryantii M.o.H. We apply our model to the experimental data presented in our companion paper on H2 thresholds. The model accurately describes H2 consumption, CH4 generation, biomass growth, substrate thresholds, and survival state during batch experiments. Methane formation stops when its Gibbs free energy is equal zero, although this does not interrupt H2 oxidation. The thermodynamic threshold for H2 oxidation occurs when the free energy for oxidizing H2 and transferring electrons to biomass is no longer negative, at approximately 0.4 nM. This threshold is not controlled by the Gibbs free energy equation of methanogenesis from H2 + HCO3- as we show in our companion paper. Beyond this threshold, the microorganisms shift to a low-maintenance metabolism called "the survival state" in response to extended H2 starvation; adding the starvation response as another new feature of the kinetic model. A kinetic threshold (or S (min)), a natural feature of the Monod kinetics, is also captured by the model at H2 concentration of around approximately 2,400 nM. S (min) is the minimum substrate concentration to maintain steady-state biomass concentration. Our model will be useful for interpreting threshold results and designing new studies to understand thresholds and their ecological implications.

Thermodynamic and kinetic analysis of the H2 threshold for Methanobacterium bryantii M.o.H.

H2 thresholds, concentrations below which H2 consumption by a microbial group stops, have been associated with microbial respiratory processes such as dechlorination, denitrification, sulfate reduction, and methanogenesis. Researchers have proposed that observed H2 thresholds occur when the available Gibbs free energy is minimal (DeltaG approximately 0) for a specific respiratory reaction. Others suggest that microbial kinetics also may play a role in controlling the thresholds. Here, we comprehensively evaluate H2 thresholds in light of microbial thermodynamic and kinetic principles. We show that a thermodynamic H2 threshold for Methanobacterium bryantii M.o.H. is not controlled by DeltaG for methane production from H2 + HCO3-. We repeatedly attain a H2 threshold near 0.4 nM, with a range of 0.2-1 nM, and DeltaG for methanogenesis from H2 + HCO3- is positive, +5 to +7 kJ/mol-H2, at the threshold in most cases. We postulate that the H2 threshold is controlled by a separate reaction other than methane production. The electrons from H2 oxidation are transferred to an electron sink that is a solid-phase component of the cells. We also show that a kinetic threshold (S(min)) occurs at a theoretically computed H2 concentration of about 2400 nM at which biomass growth shifts from positive to negative.

Kinetic characterization of Methanobacterium bryantii M.o.H.

H2 is a key electron donor for many anaerobic microorganisms; thus, keen competition for H2 occurs among H2-utilizing microbial groups. Monod kinetic parameters provide essential information for kinetic analysis of competition for H2. In this study, we estimated Monod kinetic parameter values for a methanogen that consumes only H2 as its electron donor, Methanobacterium bryantii M.o.H. Utilization of a single electron donor is an advantage in this study, because complications from alternate metabolic pathways are avoided. Using a set of batch experiments designed to provide the best estimates of each parameter, we obtained these values: maximum specific growth rate (mumax) = 0.77/ day, maximum substrate consumption rate (qmax) = 2.36 mol-H2/gcells/day, true yield (Y) = 0.325 gcell/mol H2, fraction of donor electrons to synthesis (fs degrees) = 0.03 e-cell/e- donor, half-maximum-rate substrate concentration (Ks) = 18 000 nM = 18 microM H2, and endogenous decay rate (b) = 0.088/ day. This self-consistent set of parameters indicates that, when H2 is not limiting, M. bryantii M.o.H. is a slow grower (low mumax) compared to other H2-oxidizing methanogens and sulfate reducers, and this is mainly due to its low true Y, not a low qmax. The relatively high Ks and b values suggest that M. bryantii also may not be a strong competitor when H2 is limiting.