RESUMO
The colostral cells, regarded generally as being protective, have been shown to differ in a number of membrane properties (rosetting, adherence, mobility) from the corresponding peripheral blood mononuclear cells. After in vitro stimulation with Con A or MBP 50% to 70% of the human colostral cells appeared HLA-DR positive at the first 24 h of culturing. The CD71 expression reached a maximum on culture days 2-3 coinciding with the maximal proliferative response. With regard to the phenotypic characteristics and their kinetics, the human colostral cells did not show significant differences from the peripheral blood mononuclear cells.
Assuntos
Colostro/citologia , Concanavalina A/farmacologia , Antígenos HLA-DR/biossíntese , Leite Humano/citologia , Proteína Básica da Mielina/farmacologia , Receptores da Transferrina/biossíntese , Antígenos CD/biossíntese , Antígenos de Diferenciação de Linfócitos B/biossíntese , Antígenos de Diferenciação de Linfócitos T/biossíntese , Complexo CD3 , Antígenos CD4/biossíntese , Feminino , Humanos , Receptores de Antígenos de Linfócitos T/biossíntese , Fatores de TempoRESUMO
Human colostral cells were pulsed with PHA, Con A, or LPS and cultivated in serum-free medium. The culture supernatants were tested for IL-1 activity in C3H/HeJ thymocyte assay and for IL-2 activity on human lymphoblasts. The IL-1 activity was the highest at the 24th h of cultivation and IL-2 activity at the 48th h of cultivation.