RESUMO
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood (99.5%). The specificity of CATT on diluted blood was similar (99.3%). The highest sensitivities (100%) were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood.
Assuntos
Testes de Aglutinação/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Testes de Fixação do Látex/métodos , Trypanosoma brucei gambiense/imunologia , Tripanossomíase Africana/diagnóstico , Testes de Aglutinação/economia , Testes de Aglutinação/normas , Animais , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/imunologia , Estudos de Casos e Controles , Líquido Cefalorraquidiano/parasitologia , Análise Custo-Benefício , Estudos Transversais , Doenças Endêmicas/estatística & dados numéricos , Ensaio de Imunoadsorção Enzimática/economia , Ensaio de Imunoadsorção Enzimática/normas , Hematócrito , Humanos , Testes de Fixação do Látex/economia , Testes de Fixação do Látex/normas , Linfa/parasitologia , Programas de Rastreamento , Parasitologia/economia , Parasitologia/métodos , Vigilância da População , Estudos Prospectivos , Sensibilidade e Especificidade , Sudão/epidemiologia , Tripanossomíase Africana/sangue , Tripanossomíase Africana/epidemiologia , Tripanossomíase Africana/imunologiaRESUMO
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis [CATT] on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood [99.5%]. The specificity of CATT on diluted blood was similar [99.3%]. The highest sensitivities [100%] were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood
