Lactobacillus paracasei K5 displays adhesion, anti-proliferative activity and apoptotic effects in human colon cancer cells.

Lactobacillus paracasei K5 is a lactic acid bacteria (LAB) strain, isolated recently from feta-type cheese. Its probiotic potential has been demonstrated in a series of established in vitro tests. Moreover, incorporation of L. paracasei K5 as starter culture offered organoleptic and technological advantages to novel fermented food products. In the present study, further investigation of the potential probiotic activity of L. paracasei K5 was performed and its mechanisms of action were investigated. Employing quantitative analysis and confocal, fluorescent microscopy the adhesion properties of the above strain were studied. L. paracasei K5 displayed efficient adherence capacity to Caco-2 colon cancer cells, similarly to the reference strains Lactobacillus casei ATCC 393 and Lactobacillus rhamnosus GG. Moreover, treatment of Caco-2 cells with L. paracasei K5 inhibited cell proliferation in a time-and dose-dependent manner. The anti-proliferative effects appear to be mediated through induction of apoptosis via modulation of expression of specific Bcl-2 family proteins. These results elucidate the mechanisms of action of L. paracasei K5 and enhance its potential probiotic activity.

Potentially probiotic Lactobacillus strains with anti-proliferative activity induce cytokine/chemokine production and neutrophil recruitment in mice.

Lactobacillus pentosus B281 and Lactobacillus plantarum B282 are two Lactobacillus strains previously isolated from fermented table olives. Both strains were found to possess probiotic properties and displayed desirable technological characteristics for application as starters in novel functional food production. In the present study the anti-proliferative and immunostimulatory activities of the two strains were investigated. Firstly, we demonstrated that live L. pentosus B281 and L. plantarum B282 significantly inhibited the growth of human colon cancer cells (Caco-2) in a time- and dose-dependent manner. By employing the air pouch system in mice, we showed that administration of both strains led to a rapid and statistically significant infiltration of leukocytes in the air pouch exudates. The phenotypical characterisation of the recruited immune cells was performed by flow cytometry analysis. We demonstrated that the majority of the infiltrated leukocytes were neutrophils. Finally by using the Mouse Cytokine Array Panel A Detection Antibody cocktail, we showed that both strains induced the expression of granulocyte-colony stimulating factor, interleukin (IL)-1α, IL-1ß, IL-6, chemokine (C-X-C motif) ligand (CXCL)-1, chemokine (C-C motif) ligand (CCL)-3, CCL-4, and CXCL-2 and diminished the expression levels of soluble intercellular adhesion molecule, macrophage colony-stimulating factor and metallopeptidase inhibitor 1. Our results showed that both strains display anti-proliferative and immunostimulatory properties equal or even better in some cases than those of established and commonly used probiotic strains. These findings further support the probiotic character of the two strains.

Verbal intelligence and sleep disorders in children with ADHD.

In the present study, WISC-III Verbal IQ and sleep disorders in children with Attention Deficit Hyperactivity Disorder (ADHD) and controls who were matched for age and sex were assessed. 18 children with ADHD, 14 boys and 4 girls, and 18 controls, 14 boys and 4 girls, participated. Polysomnographs were performed for all ADHD children during their night sleep. The Greek version of WISC-III was used to evaluate Verbal IQ. Analysis of the polysomnographs showed that the quality of night sleep of the children with ADHD was very poor, including apneas, low desaturation rates, and awakenings in combination with limb activity and snoring. This agreed with their low Verbal IQ, which was up to 20 points lower than the controls' Verbal IQ. Sleep disorders in ADHD children could contribute to lower Verbal IQ.

Cognitive status in Down syndrome individuals with sleep disordered breathing deficits (SDB).

Twelve subjects with Down syndrome underwent polysomnographic studies during night sleep and performed the Mini-Mental state test and the Raven Progressive Matrices (RPM), sets A, B, and B(1). Sleep-disordered breathing (SDB) deficits were observed in Down syndrome individuals and their Mini-Mental and RPM scores were extremely low. Regression analysis of the results revealed that the number of apneas per hour was related with the results of the RPM, set A, which were also related with the orientation of Mini-Mental test, indicating that the more apneas an individual has the more difficulties he has in the kind of visuoperceptual skills, including orientation, associated with normal right hemisphere functioning, which are tested by set A of the RPM.

Hemispheric asymmetries of visual ERPs in left-handed bilinguals.

ERPs (N1, P1, N2, P2) obtained from left and right occipital and temporal lobes were elicited in 30 left-handed highly proficient bilingual males in response to visually presented linguistic stimuli. The absence of statistically significant(P = < or =0.05) differences between left and right occipital and temporal lobes in all the components of the waveforms obtained, indicates a bilateral pattern of lateralization in left-handed males for both their native and foreign language.

Visual field asymmetries for rhyme and semantic tasks in fluent and nonfluent bilinguals.

A tachistoscopic study investigated hemispheric specialization among fluent and nonfluent bilinguals for rhyme and semantic tasks in both their languages. Fluent bilinguals gave faster responses and made fewer errors in their responses to the words presented in the RVF(LH) while the opposite happened for nonfluent, which indicates greater RH participation in the first stages of a second language. Fluent bilinguals performed better not only in the second but also in their native language, suggesting superior language skills on the part of fluent bilinguals. A LVF(RH) superiority was obtained for semantic tasks, indicating RH participation in semantic judgments. Another finding was a high ratio of first-born children among fluent bilinguals, potentially explained in terms of parental attitudes toward first- versus later born children.

Left-handedness and inheritance of bronchial asthma.

A statistically significant difference in mean laterality quotients of 15 asthmatic and 106 nonasthmatic children showed a coexistence of left-handedness and asthma early in life. It is suggested that this phenomenon is not only a fetal event as the Geschwind-Galaburda hypothesis claims but it is mainly based on maternal line inheritance.

A developmental study of handedness in Down syndrome pupils.

Assessed hand preference in 7- to 9-yr.-old and 13- to 15-yr.-old pupils with Down syndrome (n = 41) was compared with control schoolchildren (n = 50) of the same ages. A significant increase in left-handedness and mixed-handedness was noted in Down syndrome groups compared with the normal population. Younger Down syndrome pupils were less consistent in their hand preference than the older pupils and the normal controls.

Sex and handedness in development of visuomotor skills.

Development of visuomotor skills in 420 left-banded and 420 right-handed school children were investigated using the Rey-Osterrieth Complex Figure. Analysis indicate that the visuomotor skills involved in copying a complex figure improve with age until the mean age of 10.5 yr., in both sex and handedness groups. Further analysis showed that girls performed significantly better than boys at certain developmental stages and right-handers performed better than left-handers in various age groups. Some possible differences in performance could be attributed to different rates of maturation of the cerebral hemispheres, to different neuropsychological strategies, or to functional differences between the sex groups and between right- and left-handed children.

Visuomotor organization in the right-handed and the left-handed child: a comparative neuropsychological approach.

The present study was to assess differences between left- and right-handed children on the variable of visual-motor organization, as demonstrated by copying of a complex figure. The sample consisted of 420 right-handed and 389 left-handed children aged 5.5 to 12.5 years, who were group-matched on the basis of age, sex and hand-preference. Analysis indicated that girls show significantly better performance than boys during the ages 7.5 to 9.5, while both sex and hand-preference create a handicap for right-handed boys during ages 9.5 to 12.5. The differences in performance can be attributed to differential neuropsychological strategies or functional differences between sexes and between right and left-handed children.

Visuomotor organization in the left-handed child: a neuropsychological approach.

In this study we used the Rey-Osterrieth complex figure to examine the visuomotor organization of 514 boys and girls, aged 5.5 to 20.5 yr. All were left-handed as judged by the Edinburgh Handedness Inventory. Statistical evaluation showed significant differences in teh visuomotor organization for boys and girls across the various age groups. Statistically significant differences also appeared between the two sexes at the age groups 7.5 to 8.5 and 8.5 to 9.5, with girls showing better organization than boys.

Visuomotor organization in the child: a neuropsychological approach.

The ability of children to copy a complex figure was investigated by means of the Rey-Osterrieth complex figure and the corresponding methodology. The sample consisted of 420 children (boys and girls) aged 5 1/2 to 12 1/2 years. Responses indicate that the visuomotor ability involved in copying a complex figure increases with increasing age and that girls show significantly better performance than boys during the ages 8 1/2 to 12 1/2 years. The differences in performance can be attributed to differential neuropsychological strategies or functional differences that require further investigation.

[Hippocampus theta rhythms of wakefulness and paradoxical sleep in the rat : a comparative study]. / Rythme thêta hippocampique de veille et rythme thêta hippocampique du sommeil paradoxal chez le rat : étude comparative.

In the urethane anaesthetized Rat, hippocampal theta rhythm has been recorded during wakefulness and during paradoxical sleep under the same behavioral and postural conditions. A comparative study of these two rhythms shows the following data: 1) The wakefulness and paradoxical sleep theta rhythms significantly differ from each other in frequency and amplitude. 2) The wakefulness theta rhythm recorded in CA1 region and the paradoxical sleep theta rhythm recorded in dentate gyrus can be selectively eliminated by a septal lesion. In agreement with our previous data the present results are in favor of the existence of two kinds of theta activity having different anatomophysiological bases: one associated with wakefulness and the other with paradoxical sleep. In addition, these results suggest that the two intrahippocampal generators of theta rhythm are functionally independent.