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In recent years, the demand of consumers for products rich in protein is of significant growth. Due to its structure in tissues, protein is considered an essential nutrient for maintenance and growth. It is well known that dairy foods differ from plant-based milk alternatives in their composition. In addition to protein content, nutrients in milk and plant-based beverages vary greatly in composition and content, such as: Calcium, fiber and fat. The nutritional quality of dairy protein sources depends on both their amino acid composition and bioavailability. Indeed, dairy products are considered to be excellent sources of proteins with high Digestible Indispensable Amino Acid Score (DIAAS) values varying from 100 to 120. However, plant proteins are considered to have generally lower essential amino acid contents and lower DIAAS values than dairy proteins. For example, pea and rice proteins are known to have medium and lower DIAAS with values of 62 and 47, respectively. The present review is dedicated to study the nutritional quality of animal and plant-based milk alternatives, where a focus on protein composition and amount are determined.
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Essential oil (EO) extraction is a widespread practice generating huge amounts of solid plant by-products a potential source of bioactive compounds, on the one hand, and a detrimental risk for the environment that needs to be carefully considered on the other hand. The present study aims to valorize Myrtus communis L. leaf by-products obtained following EO extraction using a steam distillation unit through the recovery of phenolic compounds and the evaluation of their biological activities. The total phenols, flavonoids, and proanthocyanidins contents of the ethanolic extract by-product were higher than the control (leaves without extraction of EO). Their amounts increased from 69.30 to 88.06 mg GAE/g for total phenols, from 36.31 to 70.97 mg QE for flavonoids and from 19.74 to 21.49 mg CE/g of extract for proanthocyanidins. The identification of phenolic compounds by high-performance liquid-chromatography equipped with a reversed-phase (RP-HPLC) system revealed that the by-product sample includes more gallic acid, catechin, syringic acid and luteolin 7-O-glucoside but less p-coumaric acid and kaempferol than the control. Moreover, the mid-infrared spectroscopy (MIR) showed the presence of benzene ring characteristic of phenolic compounds at 756 cm-1, esters of aromatic acids and stretching vibrations of polyphenols at 1141-1234 cm-1, C=C stretching present in phenolic acids such as coumaric acid and catechin at 1604 cm-1. The assessment of antioxidant activity revealed that the ABTS+⢠radical scavenging activity was significantly increased, whereas the DPPH⢠radical inhibition activity and the ferric reduction antioxidant power were significantly decreased. The results indicated, as well, that Myrtus communis L. leaf by-products maintained a considerable antibacterial activity depending on the tested bacterial strain. Additionally, the anti-α-amylase activity was higher for the Myrtus communis L. leaf by-product extract. Therefore, Myrtus communis L. leaf by-products of EO extraction offer phenolic compounds with significant biological activities, contributing to the sustainable development and the promotion of circular economy by the recovery of valuable inputs from plant by-products.
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The effect of microfluidization treatment on the primary, secondary, and tertiary structure of soybean protein isolate (SPI) was investigated. The samples were treated with and without controlling the temperature and circulated in the system 1, 3, and 5 times at high pressure (137 MPa). Then, the treated samples were freeze-dried and reconstituted in water to check the impact of the microfluidization on two different states: powder and solution. Regarding the primary structure, the SDS-PAGE analysis under reducing conditions showed that the protein bands remained unchanged when exposed to microfluidization treatment. When the temperature was controlled for the samples in their powder state, a significant decrease in the quantities of ß-sheet and random coil and a slight reduction in α-helix content was noticed. The observed decrease in ß-sheet and the increase in ß-turns in treated samples indicated that microfluidization may lead to protein unfolding, opening the hydrophobic regions. Additionally, a lower amount of α-helix suggests a higher protein flexibility. After reconstitution in water, a significant difference was observed only in α-helix, ß-sheet and ß-turn. Related to the tertiary structure, microfluidization increases the surface hydrophobicity. Among all the conditions tested, the samples where the temperature is controlled seem the most suitable.
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Manipulação de Alimentos , Interações Hidrofóbicas e Hidrofílicas , Pós , Proteínas de Soja , Proteínas de Soja/química , Manipulação de Alimentos/métodos , Estrutura Secundária de Proteína , Temperatura , Projetos Piloto , Eletroforese em Gel de Poliacrilamida , Glycine max/química , Soluções , LiofilizaçãoRESUMO
Hydroxypropyl cellulose (HPC) is a water-soluble polymer with many applications in food, pharmaceutical, medical, or paints industries. Past studies have reported that differences in functionality can occur between products of similar pharmaceutical grades. Understanding the origin of these differences is a major challenge for the industry. In this work, the structure and physico-chemical properties of several HPC samples of the same commercial grade were studied. Structural analysis by NMR and enzymatic hydrolysis were performed to study molar substitution and distribution of substituents along the polymer chain respectively. Water-polymer interactions, surface properties as well as rheological and thermal behavior were characterized to tentatively correlate them with the structure, and gain new insights into the structure-function relationship of this polymer. The differences in structure revealed between the samples affect their properties. The unexpected behavior of one sample was attributed to a more heterogeneous substitution pattern, with the coexistence of highly and weakly substituted regions along the same polymer chain. The more block-like distribution of substituents has a great effect on the clouding behavior and surface tension reduction ability of the polymer.
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Pesticide residues in vegetables are potentially toxic components to humans and can cause serious health problems. To remove pesticide residues from fresh agricultural products and improve consumer food safety, various pesticide removal methods have been investigated over the past decades. In this study, the effectiveness of laboratory and pilot scale ultrasonic cleaning on the removal of boscalid and pyraclostrobin residues from lettuce was examined. 2D fluorescence spectroscopy, 3D fluorescence spectroscopy represented by excitation-emission matrix (EEM), and parallel factor analysis (PARAFAC) were used to characterize and discriminate the fluorescence signatures of these pesticides in the cleaning water to determine the effectiveness of the ultrasonic cleaning method as a function of the level of pesticide removal. The 2D fluorescence results showed that the rate of removal of boscalid by ultrasonics at the laboratory scale increased with the cleaning time. The ultrasonic treatment showed a higher cleaning efficiency compared to only soaking in distilled water for 10 min. The same trends were observed at the pilot scale. The EEM also showed differences in the concentration of pesticides removed by ultrasonication between the different parts of the lettuce, the concentration was higher in the upper part than the lower part. This study showed that ultrasonication is an effective technique for the removal of pesticide residues on lettuce, and it also showed the significant potential of fluorescence spectroscopy coupled with PARAFAC for the discrimination and characterization of pesticides.
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Resíduos de Praguicidas , Praguicidas , Humanos , Resíduos de Praguicidas/análise , Lactuca/química , Espectrometria de Fluorescência/métodos , Praguicidas/análise , Água/químicaRESUMO
This work aimed at studying the influence of formulation and storage on the formation of Maillard compounds called 5-hydroxymethylfurfural (HMF) and furfural in pound cakes formulated with rapeseed oil (RO) and palm oil (PO). A progressive humidification of the crust and a dryness of the crumb were observed during storage. Lightness (L*) decreased for both PO and RO pound cakes in crumb as well as crust. The accumulation of HMF was found to be higher in the crust (2.21-38.5 mg kg-1) in comparison with the crumb (0.78 to 10.29 mg kg-1). Similar results were found for furfural where the concentration range was between 0.98 and 5.67 mg kg-1 in the crumb while between 2.1 and 38.39 mg kg-1 in the crust. Thus, the formation of HMF and furfural depended on formulation. The PLS-DA showed a positive correlation between the kinetic of HMF and furfural migration and the storage period.
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Furaldeído , Óleo de Brassica napus , Óleo de Palmeira , CinéticaRESUMO
Sausages are among the most vulnerable and perishable products, although those products are an important source of essential nutrients for human organisms. The evaluation of the quality of sausages becomes more and more required by consumers, producers, and authorities to thwarter falsification. Numerous analytical techniques including chemical, sensory, chromatography, and so on, are employed for the determination of the quality and authenticity of sausages. These methods are expensive and time consuming, and are often sensitive to significant sources of variation. Therefore, rapid analytical techniques such as fluorescence spectroscopy, near infrared (NIR), mid infrared (MIR), nuclear magnetic resonance (NMR), among others were considered helpful tools in this domain. This review will identify current gaps related to different analytical techniques in assessing and monitoring the quality of sausages and discuss the drawbacks of existing analytical methods regarding the quality and authenticity of sausages from 2015 up to now.
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Produtos da Carne , Humanos , Produtos da Carne/análiseRESUMO
The production of biscuit and biscuit-like products has faced many challenges due to changes in consumer behavior and eating habits. Today's consumer is looking for safe products not only with fresh-like and pleasant taste, but also with long shelf life and health benefits. Therefore, the potentiality of the use of healthier fat and the incorporation of natural antioxidant in the formulation of biscuit has interested, recently, the attention of researchers. The determination of the biscuit quality could be performed by several techniques (e.g., physical, chemical, sensory, calorimetry and chromatography). These classical analyses are unfortunately destructive, expensive, polluting and above all very heavy, to implement when many samples must be prepared to be analyzed. Therefore, there is a need to find fast analytical techniques for the determination of the quality of cereal products like biscuits. Emerging techniques such as near infrared (NIR), mid infrared (MIR) and front face fluorescence spectroscopy (FFFS), coupled with chemometric tools have many potential advantages and are introduced, recently, as promising techniques for the assessment of the biscuit quality.
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Antioxidantes , AlimentosRESUMO
In this study, the potentiality of front face fluorescence spectroscopy (FFFS) for the evaluation of the quality of biscuits manufactured with butylated hydroxytoluene and pomegranate peel extract during aging was investigated. By using the principal component analysis, vitamin A and tryptophan spectra allowed a clear discrimination between biscuit samples according to the nature of antioxidants, while fluorescent Maillard reaction products spectra showed clear differentiation between samples according to the storage time. Clear differentiation between biscuits according to the used antioxidants and storage time was achieved by using common components and specific weights analysis. Using partial least-squares regression, excellent prediction of water activity (R 2 = 0.95), and L* values (R 2 = 0.92), and approximate prediction of hardness (R 2 = 0.78), b* values (R 2 = 0.74), and moisture content (R 2 = 0.74) were shown. However, the FFFS failed to predict a* values, primary and secondary oxidation products (R 2 < 0.6).
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The viscoelastic and the structure properties of three brands of dry sausages (Auvergne, Beef-poultry, and Galbanetto) were studied using dynamic rheology and fluorescence spectroscopy. The storage and the loss modulus of sausage samples showed a viscoelastic character. The principal component analysis carried out on the normalized rheological and physico-chemical parameters allowed to differentiate the sausage samples into three groups according to their brands. This trend was confirmed by the factorial discriminant analysis where 74.44% of correct classification was obtained. The emission spectra acquired after excitation set at 290, 340, and 360 nm and excitation spectra scanned after emission set at 410 nm allowed clear differentiation between the three brand samples. The obtained results were confirmed following the application of partial least squares regression to the fluorescence and physico-chemical parameters since an excellent prediction of moisture content was obtained from the excitation spectra set at 340 nm (R2 = 0.99) and 360 nm (R2 = 0.99). The protein content of dry sausages was well predicted after excitation set at 290 and 340 nm with R2 of 0.96 and 0.97, respectively, while the fat level was well estimated after excitation set at 340 and 360 nm and emission set at 410 nm (R2 = 0.96, 0.96 and 0.94, respectively). The obtained results showed the potential use of fluorescence spectroscopy as a rapid technique for evaluating the quality of dry sausages.
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Produtos da Carne , Animais , Bovinos , Fenômenos Químicos , Análise Discriminante , Análise dos Mínimos Quadrados , Produtos da Carne/análise , Reologia , Espectrometria de Fluorescência/métodosRESUMO
BACKGROUND: This study aimed to investigate the effects of the supplementation of sesame flour in fermented chicken sausages ('S1' containing 800 g kg-1 chicken fillet, 180 g kg-1 veal fat and 20 g kg-1 sesame flour and 'S2' containing 800 g kg-1 chicken fillet, 160 g kg-1 veal fat and 40 g kg-1 sesame flour) compared with control sausages (containing 800 g kg-1 chicken fillet and 200 g kg-1 veal fat) on the physico-chemical characteristics, texture, and structure during the fermentation stage. RESULTS: The physicochemical parameters of samples belonging to the control, S1, and S2 batches were significantly affected by the addition of sesame flour and the fermentation stage. For instance: (i) the lowest protein content was observed for control samples on day 1 (61.4 ± 6.52 g kg-1 ) whereas the highest level was noted for S2 samples on day 15 (327.5 ± 22.2 g kg-1 ), and (ii) an inverse trend was observed for the fat content because the lowest content was observed for samples in the S2 batch on day 1 (129.0 ± 5.30 g kg-1 ) whereas the highest fat content was noted for samples belonging to control batch on day 15 (332.0 ± 1.29 g kg-1 ). The application of statistical methods to mid-infrared spectroscopy allowed clear discrimination between control, S1, and S2 batches. The addition of sesame flour in the recipes induced some modification in the secondary structure because ß-turn levels ranged from 39.30 to 34.50, 36.76 to 34.70, and 38.93 to 34.70 for control, S1, and S2 batches, respectively, throughout the fermentation stage. Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a similar protein profile pattern in the three batches on days 1 and 5, but on day 10 control and S2 batches showed the most intense degradation of myofibrillar proteins. CONCLUSION: The results demonstrated that mid-infrared spectroscopy coupled with chemometric tools could be used as a rapid screening tool to assess and monitor the quality of dry chicken sausages enriched with sesame flour throughout the fermentation stage. © 2022 Society of Chemical Industry.
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Produtos da Carne , Sesamum , Animais , Bovinos , Farinha/análise , Galinhas , Produtos da Carne/análise , Análise EspectralRESUMO
This study aims to evaluate the impact of incorporating pear, date, and apple by-products on pasta properties. Pasta properties including cooking quality, texture, color, rheology, thermal gelling, and microstructural characteristics were evaluated. Common wheat flour was substituted by 0, 2.5, 5, 7, and 10 g/100 g of by-products. To choose the best-suited substitute of flour for the preparation of pasta, the sensorial properties of pasta were investigated. Interrelationships between all the physicochemical parameters were investigated using multiple factor analysis. We also studied the impact of storage (7, 15, and 30 days) on the physicochemical proprieties of pasta. The results revealed that the chemical composition of pasta elaborated with by-products was characterized by higher energy (~386 Kcal) and fiber content (~13%) than the control pasta. Generally, materials added to the durum wheat pasta reduce optimum cooking time, adhesiveness, and extensibility, and enhance the swelling index, cooking loss, cooking water absorption, water activity, firmness, and tenacity of pasta. Cooked pasta samples were significantly (p < 0.05) darker (L*) and greener (-a*) than the control pasta. Increasing the rate of by-products from 2.5% to 10% principally altered the texture and structure of pasta. Scanning electron microscopy analysis showed that the inclusion of by-products into pasta leads to a disruption of the protein matrix. A practical formulation (2.5% of by-products) can be selected, since a significant difference was detected between overall acceptability scores. Grouping the variables in the principal component analysis plot showed that pasta samples can be divided into three groups. Each group was correlated by a specific variable. A significant modification of the physical parameters of pasta was observed after 30 days of storage.
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Two vegetal oils have been selected for the formulation of pound cakes, rapeseed oil (RO) and palm oil (PO). The freshness of these products has been monitored during 58 days of storage. The RO pound cakes of 58 days as well as PO pound cakes presented higher values (p < .05) of hardness (N), Young's modulus (N.cm-2 ) and compressive stress (N.cm-2 ) than those aged of 5 days. For both vitamin A and tryptophan spectra, the maximum of fluorescence intensity varied according to the aging period. For both RO and PO recipes, lipid oxidation indicators (peroxide value, para-anisidine value, and total oxidation value [TOTOX]) were interestingly correlated to Young's modulus (R2 = 0.99) as well as compressive stress (R2 = 0.99), using polynomial model. The application of partial least squares regression to the vitamin A spectra provided excellent predictive results for both compressive stress (R2 = 0.99) and TOTOX (R2 = 0.99). Good results were observed for tryptophan spectra for the prediction of TOTOX (R2 = 0.77). Thus, the use of front face fluorescence spectroscopy may provide rapid and accurate results for the monitoring of pound cakes freshness throughout storage.
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Quimiometria , Triptofano , Espectrometria de Fluorescência , Vitamina ARESUMO
This study investigates the valorization of the nettle leaves (Urtica dioica) as a novel source of a protease for clotting dromedary milk. The aim of this work is to study the effect of extracting pH on the enzymatic activity of nettle leaves extracts. The extraction was achieved in phosphate citrate buffer at different pH values (from 3 to 6.5) and the obtained extracts were used to coagulate dromedary milk. The characterization of the obtained extracts was carried out using non-destructive methods namely FT-MIR, fluorescence spectroscopy and turbiscan instrument. The extract prepared at pH = 4 had the highest proteolytic activity. The fluorescence and turbiscan measurements revealed a substantial effect of the pH value on chlorophyll residues extraction and stability, respectively. At an acidic environment (pH range of 3 - 4), the enzymatic extracts were unstable (with turbiscan stability index (TSI) values ~ 20), while at a nearly neutral pH value (pH range of 5 - 6.5), they were found to be more stable as indicated by the low TSI values ~ 1. The maximum milk-clotting activity (MCA) (0.021 U/mL) was obtained for the extracts prepared at pH = 4.
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Urtica dioica , Animais , Camelus , Leite , Peptídeo Hidrolases , Extratos Vegetais , Folhas de Planta/química , Urtica dioica/químicaRESUMO
The determination of the quality and authenticity of olive oil becomes more and more required by producers, consumers, and authorities to thwarter falsification. Several analytical techniques including chemical, sensory, chromatography, and so on, are used for the determination of the quality and authenticity of olive oil. Although these methods are considered as the reference ones, they are cumbersome, time-consuming and destructive. Therefore, rapid analytical techniques such as fluorescence, ultraviolet-visible, near infrared, and mid infrared spectroscopies, electronic sensing, among others, are more and more used for the determination of the quality and authenticity of olive oils. This review will identify current gaps related to different analytical techniques in olive oil authentication and discuss the drawbacks of existing analytical methods concerning olive oil authenticity from 2010 up to now.
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Óleos de Plantas , Azeite de Oliva/químicaRESUMO
This study examines the ability of fluorescence spectroscopy for monitoring the quality of 70 Moroccan virgin olive oils belonging to three varieties and originating from three regions of Morocco. By applying principal component analysis and factorial discriminant analysis to the emission spectra acquired after excitation wavelengths set at 270, 290, and 430 nm, a clear differentiation between samples according to their storage time was observed. The obtained results were confirmed following the application of four multivariate classification methods: partial least squares regression, principal component regression, support vector machine, and multiple linear regression on the emission spectra. The best prediction model of storage time was obtained by applying partial least squares regression since a coefficient of determination (R2) and a root mean square error of prediction (RMSEP) of 0.98 and 24.85 days were observed, respectively. The prediction of the chemical parameters allowed to obtain excellent validation models with R2 ranging between 0.98 and 0.99 for free acidity, peroxide value, chlorophyll level, k232, and k270.
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Óleos de Plantas , Análise Discriminante , Análise dos Mínimos Quadrados , Azeite de Oliva/análise , Análise de Componente Principal , Espectrometria de FluorescênciaRESUMO
The present study was attempted to investigate the effect of succinylation, as chemical modification, on the functionality of male date palm pollen protein concentrate (MDPPPC). Succinylation was applied at two levels, 4 and 8 mol of succinic acid per mole of lysine. 4 M and 8 M were compared to the native MDPPPC. Findings proved that succinylation improved the surface properties of pollen protein including solubility and surfactant activity. Increased solubility of succinylated MDPPPC has been noticed especially in pH superior to pHi. The results from the differential scanning calorimetry showed a significant decrease (P < 0.05) of the denaturation temperature and the heat enthalpy for succinylated MDPPPC. ß turn of succinylated MDPPPC increased significantly (P < 0.05) at the expense of ß sheet indicating that the protein gained more mobility after succinylation which explains the enhancement of the functional properties and promotes the use of succinylated protein as a techno-functional ingredient.
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This study examines the feasibility of using front face fluorescence spectroscopy (FFFS) to authenticate 41 virgin olive oil (VOO) specimens collected from 5 regions in Morocco (Fez/Meknes, Eastern, Northern, Beni-Mellal/Khenifra, and Marrakech/Safi) during 2 consecutive crop seasons (2015-2016 and 2016-2017). By jointly applying factorial discriminant analysis (FDA) to the emission spectra acquired after excitation at 270, 290, and 430 nm, clear discrimination between VOOs according to their geographic origin (96.72% correct classification) and variety (95.12% correct classification) was observed. This trend was confirmed following the application of partial least squares regression (PLSR) to the fluorescence spectra, where excellent prediction of free acidity (R2 = 0.98) and peroxide (R2 = 0.96) values and good prediction of k232 (R2 = 0.88), k270 (R2 = 0.88), and chlorophyll content (R2 = 0.89) values were observed.
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BACKGROUND: Olive oil provides a wide range of health-promoting compounds. The quality of olive oil is an even more complex concept as it is affected by several factors, such as variety, season, stage of maturation, extraction processing, and so on. The main objective of this study was to determine the potential of chemical and mid-infrared spectroscopy techniques to determine the quality and authenticity of virgin olive oil (VOO). For this, we studied 41 VOOs originating from five regions of Morocco (Fez/Meknes, Eastern, Northern, Beni-Mellal/Khenifra, and Marrakech/Safi) and produced using different agricultural and technological conditions during two successive crop seasons (2015-2016 and 2016-2017). RESULTS: By applying principal component analysis and factorial discriminant analysis with leave-one-out validation to the mid-infrared spectroscopy, clear discrimination between VOO samples according to their geographic origin and variety was observed, with correct classification rates of 91.87% and 91.87% being observed respectively. The application of partial least-squares regression to mid-infrared and chemical data sets allowed excellent prediction of free acidity, peroxide value, k270 , and chlorophyll level with R2 of 0.99, 0.97, 0.98, and 0.93 respectively, and good prediction of k232 (R2 = 0.84). CONCLUSION: The results demonstrate that mid-infrared spectroscopy coupled with chemometric tools could be used as a rapid screening tool for evaluating the overall quality and authenticity of VOO. © 2020 Society of Chemical Industry.
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Análise de Alimentos/métodos , Azeite de Oliva/análise , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Clorofila/análise , Análise Discriminante , Marrocos , Azeite de Oliva/classificação , Análise de Componente PrincipalRESUMO
The physico-chemical, textural and color parameters and fluorescence spectra of aromatic amino acids and nucleic acids (AAA + NA), tryptophan residues, nicotinamide adenine dinucleotide (NADH) and vitamin A were scanned on sturgeon samples kept at 4 °C up to 12 days. Using the principal component analysis (PCA), the riboflavin and NADH spectra allowed clear differentiation between sturgeon samples according to their storage time. The best result was obtained by applying the common components and specific weights analysis (CCSWA) to the riboflavin and NADH data tables since better differentiation was achieved between the 4 group sturgeon samples aged: 2 days; 5, 6 and 7 days; 8 and 9 days and 12 days. The proposed methodology demonstrates the ability of CCSWA to evaluate sturgeon freshness level according to their storage time.